THE ROLE OF SYMPATHIC AND PARASIMPATIC INNERVATION IN NEUROIMMULAR INTERACTIONS WITH EXTERNAL GUINETAL ENDOMETRIOSIS

Currently the study of the role of peripheral nervous system in control of inflammation in endometriosis and its involvement in the development of the chronic pain syndrome in this pathology is of relevance. The purpose of this work was to assess the reciprocal location of sympathetic and parasympathetic nerve fibers with external genital endometriosis at various stages of progression. The samples for the study were the endometrial biopsies of patients of reproductive age with external genital endometriosis of I-IV stage of the disease, with primary sterility (n = 20). The control group consisted of 3 patients (n = 5) who did not have any signs of endometriosis. Molecular markers were visualized using the immunohistochemical method. Antibodies to tyrosine hydroxylase (TH, 1:200, Abcam, USA) and PGP 9.5 (PGP 9.5, 1:1000, Abcam, USA) were used as primary antibodies. Alexa Fluor 488 (1:1000, Abcam, USA) and Alexa Fluor 647 (1:1000, Abcam) were used as secondary antibodies. The results were visualized with a confocal microscope (FluoView1000 (Olympus)). 3D shooting was used to assess the reciprocal location of the studied markers. As a result of this work, it was found that the expression of both of studied markers was detected in all samples. The obtained data can significantly increase the understanding of the functioning mechanisms of the neoneurogenesis processes in ectopic and eutopic tissue, thereby allowing an increase in the accuracy of the endometriosis diagnostic methods.

endometriosis, tyrosine hydroxylase, PGP9.5, nerve fibers, confocal microscopy, PGP9.5