COMPARATIVE EFFECT OF KE AND EW PEPTIDES ON NORMAL AND TUMOR IMMUNE CELLS

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Abstract

KE and EW peptides have an immunoprotective and oncostatic effect in experimental studies. However, their comparative effect on normal and cancer immune cells has not been carried out yet. The aim of the work is to study the effect of KE and EW peptides on T-lymphocytes and Berkitt lymphoma cells growth in culture. To plot the cell growth curve, the cells were scattered at a concentration of 20,000 per 2 ml. KE and EW peptides were added to cultures at 100 ng/ml. Counting cells was carried out in the Gorjaev’s chamber. KE and EW peptides increased the number of lymphocytes of human blood as compared with the control during 5 days by 64-219%. KE and EW peptides reduced the number of Burkitt lymphoma cells as compared with the control during 5 days by 37-101%. KE and EW peptides decreased tumor immune cell proliferation and increased normal human lymphocytes grow. We suppose, that these effects can be connected with various ways of peptide regulation of methylation status of DNA.

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About the authors

N S Linkova

Saint Petersburg Institute of Bioregulation and Gerontology, Saint Petersburg; Academy of Postgraduate Education under FSBU FSCC of FMBA of Russia, Moscow

E S Mironova

Saint Petersburg Institute of Bioregulation and Gerontology, Saint Petersburg

O M Ivko

Saint Petersburg Institute of Bioregulation and Gerontology, Saint Petersburg

O A Orlova

Saint Petersburg Institute of Bioregulation and Gerontology, Saint Petersburg

A V Dudkov

Saint Petersburg Institute of Bioregulation and Gerontology, Saint Petersburg

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Copyright (c) 2019 Linkova N.S., Mironova E.S., Ivko O.M., Orlova O.A., Dudkov A.V.

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