Characteristics of the functional state of peripheral blood neutrophils in patients with luminal breast cancer



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Abstract

Aim - To analyze the chemotactic activity of neutrophils and the tendency to NETosis in blood samples of patients with locally advanced luminal breast cancer (BC) undergoing treatment (neoadjuvant chemotherapy) at the Moscow Medical Research Center named after A.S. Loginov. Materials and Methods. The study was conducted on blood samples from six patients with stage III luminal B, HER 2-negative breast cancer before and two months later on antitumor therapy. Blood samples from healthy adult volunteers were used as a control. The work was performed using fluorescence microscopy methods for neutrophil chemotaxis with the growth of blood clots and the number of NETs by reaction with Hoechst 33042 and antibodies against myeloperoxidase (MPO) and neutrophil elastase (NE) in smears of leukocyte-rich blood plasma. Results. In patients with breast cancer, the level of NETosis was significantly increased (30±14% vs. 4.6±3.4% in healthy donors), while most patients on therapy had a decrease in the level (17±17%). Neutrophil movement velocities also increase in some patients (0.17±0.06 µm/s vs. 0.113±0.009 µm/s in healthy donors) and decrease during therapy (0.10±0.03 µm/s). At the same time, the number of neutrophils associated with blood clots decreases during therapy (25±18 vs. 61±23) even in patients with neutrocytosis. Conclusion. It was shown that in patients with breast cancer the rate of neutrophil chemotaxis deviates from the control; at the same time, their adhesion is reduced, and peripheral blood neutrophils are significantly more prone to NET formation than those from healthy donors.

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About the authors

Julia Jessica D. Korobkin

Email: juliajessika@gmail.com

Ekaterina Iva А. Adamanskaya

Email: ka.09@mail.ru

Natalya I. Polshina

Loginov Moscow Clinical Scientific Center

Email: npolshina@yandex.ru
ORCID iD: 0000-0001-5417-0425

oncologist

Russian Federation, Moscow

Sofia V. Galkina

Email: s_v_galkina@rambler.ru

Timur I. Kadyrov

Email: kadyrov.ti17@physics.msu.ru

Nikolay P. Gorbunov

Institute of Experimental Medicine

Email: niko_laygo@mail.ru
ORCID iD: 0000-0003-4636-0565
SPIN-code: 6289-7281

Research fellow of the Department of Molecular Genetics

Russian Federation, Saint-Petersburg

Alexey V. Sokolov

Institute of Experimental Medicine; Saint Petersburg State University

Email: biochemsokolov@gmail.com
ORCID iD: 0000-0001-9033-0537
SPIN-code: 7427-7395

Doctor of Biological Sciences, Head of the Laboratory of Biochemical Genetics of the Department of Molecular Genetics, Professor of Chair of Fundamental Problems of Medicine and Medical Technology

Russian Federation, Saint-Petersburg

Lyudmila G. Zhukova

Loginov Moscow Clinical Research Center

Email: zhukova.lyudmila008@mail.ru
ORCID iD: 0000-0003-4848-6938

D. Sci. (Med.)

Russian Federation, Moscow

Anastasia N. Sveshnikova

Dmitry Rogachev National Research Center of Pediatric Hematology, Oncology, and Immunology, Moscow, Russia

Author for correspondence.
Email: a.sveshnikova@physics.msu.ru

Dr. Habil, Head of cell biology and translational medicine lab

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