Morphological differences in the commissural connections of the forebrain in white outbred mice and BALB/C mice
- Authors: Karpova I.V.1, Popkovsky N.A.1,2, Proshin S.N.3, Bychkov E.R.3, Tissen I.Y.3, Droblenkov A.V.1,2
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Affiliations:
- The Institute of Experimental Medicine
- Saint Petersburg Medicо-Social Institute
- Institute of Experimental Medicine
- Issue: Vol 21, No 2 (2021)
- Pages: 99-105
- Section: Original research
- Published: 24.09.2021
- URL: https://journals.eco-vector.com/MAJ/article/view/71636
- DOI: https://doi.org/10.17816/MAJ71636
- ID: 71636
Cite item
Abstract
BACKGROUND: The study of the mechanisms of interaction of paired structures of the mammalian brain is a fundamental problem of modern neuroscience, which is of great applied importance. Even mild underdevelopment of the corpus callosum in humans can lead to autism. It is known that the intensity of intraspecific interactions in BALB/c mice is lower than in white outbred ones, while some BALB/c substrains are characterized by underdevelopment of the corpus callosum.
AIM: To compare the morphological parameters of the large brain commissures in white outbred mice and BALB/c mice grown in the Rappolovo nursery (Leningrad region).
MATERIALS AND METHODS: The morphology of the corpus callosum was studied in 13 male white outbred mice and 7 male BALB/c mice at the age of 8 months.
RESULTS: In mice of both subpopulations, the area of the anterior commissure of the left hemisphere was smaller than that of the right hemisphere (p < 0.05). There were no differences between subpopulations in this parameter. The area of the left section of the corpus callosum trunkus in outbred mice was larger than the right one (p < 0.001), while in BALB/c mice the areas of the left and right slices did not differ. Despite the absence of significant differences in the area of the anterior part (rostrum et genu) of the corpus callosum the density of the location of oligodendrocytes in this brain structure in the mice of the two subpopulations was different. The number of oligodendrocytes in 0.01 mm2 on the left section of the anterior part of the corpus callosum in BALB/c mice was greater than in white outbred mice (p < 0.05). A similar trend was revealed when comparing slices of the right hemisphere (p = 0.065).
CONCLUSIONS: The large area of the right parasagittal slice of the anterior commissure suggests that some of its constituent fibers do not cross the midline, but end within the same hemisphere, which may be the morphological basis for the functional dominance of the temporal cortex of the left hemisphere in mice of both subpopulations. The corpus callosum in BALB/c mice is developed symmetrically, and in white outbred ones – asymmetrically. This feature may be the morphological basis for the functional dominance of the parietal cortex of the right hemisphere in outbred animals.
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About the authors
Inessa V. Karpova
The Institute of Experimental Medicine
Email: inessa.karpova@gmail.ru
ORCID iD: 0000-0001-8725-8095
SPIN-code: 9874-4082
PhD (Physiology), Senior Researcher, Department of Neuropharmacology named after S.V. Anichkov
Russian Federation, Saint PetersburgNikita A. Popkovsky
The Institute of Experimental Medicine; Saint Petersburg Medicо-Social Institute
Email: popkowskij.nikita@yandex.ru
Postgraduate student
Russian Federation, Saint PetersburgSergey N. Proshin
Institute of Experimental Medicine
Email: psnjsn@rambler.ru
Dr. Sci. (Med.), Professor
Russian Federation, Saint PetersburgEvgeny R. Bychkov
Institute of Experimental Medicine
Email: bychkov@mail.ru
ORCID iD: 0000-0002-8911-6805
SPIN-code: 9408-0799
PhD (Pathophysiology), Cand. Sci. (Med.)
Russian Federation, Saint PetersburgIlia Y. Tissen
Institute of Experimental Medicine
Email: iljatis@mail.ru
ORCID iD: 0000-0002-8710-9580
SPIN-code: 9971-3496
PhD, Cand. Sci. (Biol.), Senior Researcher
Russian Federation, Saint PetersburgAndrey V. Droblenkov
The Institute of Experimental Medicine; Saint Petersburg Medicо-Social Institute
Author for correspondence.
Email: droblenkov_a@mail.ru
ORCID iD: 0000-0001-5155-1484
SPIN-code: 8929-8601
Scopus Author ID: 6507854101
Dr. Sci. (Med.), Leading Researcher of the Department of Neuropharmacology named after S.V. Anichkov, Head of the Department of Biomedical Disciplines
Russian Federation, Saint PetersburgReferences
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