ЭКСПРЕССИЯ ГЕНА БЕТА-ДЕФЕНСИНА-3 ЧЕЛОВЕКА В СЛИЗИСТОЙ ОБОЛОЧКЕ НОСА И ОКОЛОНОСОВЫХ ПАЗУХ

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The immune system’s ability to recognize and communicate information to the nervous system about the presence of viruses and bacteria has earned it a label as the so-called sixth sense [3]. Sensitive receptors of the olfactory sensory system are located in the nasal cavity mucosa. The first line of the respiratory tract defense is represented by the airway epithelium, a pseudostratified epithelium of basal cells, ciliated cells, and goblet cells [5]. The epithelial cell layer contributes greatly to nasal and sinonasal innate immunity through physical barrier function, mucociliary clearance, and secretion of antimicrobial products. The aim of this study was to evaluate the human beta-defensin-3 (hBD-3) gene expression in the surface epithelium of the nasal and sinonasal mucosa. Material and methods. Mucosal samples were obtained from 85 adult patients with nasal and sinonasal disease undergoing nasal septum operation, functional endoscopic sinus surgery and inferior turbinate reduction under general anaesthesia, and classified according to the clinical diagnoses and the anatomical regions. Seven groups were defined; healthy tissue of the middle nasal passage mucosa and normal inferior turbinate mucosa that served as controls (table 1). Mucosal biopsy specimens were taken intraoperatively from the nasal or sinonasal cavity. The mucosal tissues were preserved immediately in 0.2 mL of RNAlater (Ambion). The tissue samples were stored in RNAlater at -20 °C until they were processed for RNA isolation. Mucosal samples were thoroughly grinded with a mortar and pestle and homogenized using Pellet Pestle Motor (Kontes) (Sigma-Aldrich). Total RNA was extracted from the superficial epithelium of mucosa using the Gen Elute Mammalian Total RNA Miniprep Kit (RTN70) and On-Column DNase I Digestion Set (DNASE70) according to the manufacturer’s instructions (Sigma-Aldrich). Total RNA of 2 mg was reverse transcribed to generate cDNA with M-MLV RT (Promega) in the presence of oligo(dT), RNasin® and dNTPs (Medigen). Real-time polymerase chain reaction (RT-PCR) was performed using iQTM SYBR Green Supermix (Bio-Rad) and specific primers (sense 5´-tatcttctgtttgctttgctcttcc-3’ and antisense 5´-cctctgactctgcaataatatttctgtaat-3’) [1], and measured with CFX-96 Touch™ and software CFX Manager™ version 2.1 (Bio-Rad). Reactions were incubated for 5 min at 95 °C, followed by 40 cycles of a two-step amplification-procedure composed of annealing/extension at 60 °C for 1 min and denaturation for 10 s at 95 °C. Plate read was performed at 72 °C. Specificity reaction products were evaluated by melt curve. Melt temperature of the amplification products was 78 °С for hBD-3 and 88 °С for beta-actin. The relative hBD-3 mRNA expression of the samples was standardized using software with beta-actin mRNA as a housekeeping control (sense 5’-gggtcagaaggattcctatg-3’, antisense 5’-ggtctcaaacatgatctggg-3’). Statistical analysis was performed using GraphPad Prism 5 software. Distribution of the samples was tested with the Kolmogorov-Smirnov test. Comparison of numerical data between groups was first established using the Kruskal-Wallis test, then the Wilcoxon signed rank test. Comparison of proportions between groups was carried out using Fisher’s exact test and odds ratio (OR). P-values less than 0.05 were considered statistically significant. Results and discussion. The expression of hBD-3 gene was detected in all defined anatomical regions at low levels, but it was absent in the hypertrophic inferior turbinate mucosa (p < 0.05 compared to the middle nasal passage mucosa; p < 0.01, OR 31.15, 95% confidence interval (CI) 1.53÷633.6 compared to the middle nasal passage polyps) (table 1). The highest detection frequency of hBD-3 mRNA expression was detected in the middle nasal passage polyps (p < 0.05, OR 7.00, CI 1.10÷44.63 compared to the sinus maxillaries mucosa). The highest levels of hBD-3 gene expression was detected in the middle nasal passage polyps also (p < 0.05 compared to the hypertrophic inferior turbinate mucosa). The epithelial cells of the nose and paranasal sinuses must maintain an adequate mucosal defense system against invading pathogens and various antigenic stimuli. The innate immune response provides protection immediately after an infectious challenge. The nasal and sinonasal epithelium contains a chemical defense shield through the expression and secretion of various antimicrobial peptides [4]. Protecting the upper airway from microbial infection is an important function of the immune system [3]. Clinically, inflammatory polyps are found in the middle turbinate in patients with chronic rhinosinusitis but not in the inferior turbinate [6]. Excessive activity of the immune system can cause self-damage of the host by immunopathological processes [5]. hBD-3-stimulated TLR1/2 activation induces a pro- rather than an anti-inflammatory cytokine pattern in monocytes [2]. Conclusions. Our findings suggested that epithelial in the nasal and sinonasal mucosa were a physical barrier, which could represent a defense mechanism without severe inflammation. Expression of hBD-3 in proximity to areas of cellular dysregulation may inadvertently exacerbate disease progression. In the context of chronic inflammation, apart from direct antimicrobial activity, high concentrations hBD-3 also potentially contributes to epithelial injury and fibrotic remodeling.

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Е В Тырнова

ФГБУ «Санкт-Петербургский научно-исследовательский институт уха, горла, носа и речи» МЗ РФ

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