Genetically modified legume plants as a basis for studying the signal regulation of symbiosis with nodule bacteria
- Авторы: Dolgikh E.A.1, Kantsurova E.S.1, Kozyulina P.Y.1, Bovin A.D.1, Dolgikh A.V.1,2, Dymo A.M.1, Kozlov N.V.1,2
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Учреждения:
- All-Russia Research Institute for Agricultural Microbiology
- Saint Petersburg State University
- Выпуск: Том 21 (2023): Спецвыпуск
- Страницы: 54-55
- Раздел: «ГМО: ИСТОРИЯ, ДОСТИЖЕНИЯ, СОЦИАЛЬНЫЕ И ЭКОЛОГИЧЕСКИЕ РИСКИ»
- URL: https://journals.eco-vector.com/ecolgenet/article/view/568623
- DOI: https://doi.org/10.17816/ecogen568623
- ID: 568623
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Аннотация
The development of legume-rhizobium symbiosis is based on signal exchange between partners, which leads to the formation of nitrogen-fixing root nodules. Under the influence of rhizobial signal molecules, the Nod factors, the signal transduction cascade is activated, where the LysM-type receptor kinases and a complex of intracellular regulators, a significant part of which are still unknown, can play an important role.
Using transcriptomic and proteomic analysis, we searched for new regulators of the signal pathway in pea Pisum sativum L., which are activated under the influence of Nod factors. Phospholipases C and D, GTPases, calcium-dependent protein kinases, and mitogen-activated protein kinases (MAPKs) have been identified among such novel regulators. The influence of one of such regulators, the MAPK6, on the development of symbiosis was studied in more detail. Using genetic engineering approaches, we increased the transcriptional activity of MAPK6 in transgenic roots, which led to an increase in the number of nodules and the biomass of pea plants. A similar effect was also found for the homologous MAPK6 gene in another legume Medicago truncatula, which has a type of nodulation similar to that one in pea. New approaches have been developed to obtain stable pea transformants with enhanced level of MAPK6 transcription using the constitutive p35S promoter. A comprehensive study of such plants inoculated with rhizobia was carried out. In addition, the approaches for genome editing of pea plants have been designed using the CRISPR/Cas system, when the MAPK6 gene was used as a target. Thus, the possibility of using genetic engineering methods to obtain plants with increased symbiosis efficiency was investigated.
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Полный текст
The development of legume-rhizobium symbiosis is based on signal exchange between partners, which leads to the formation of nitrogen-fixing root nodules. Under the influence of rhizobial signal molecules, the Nod factors, the signal transduction cascade is activated, where the LysM-type receptor kinases and a complex of intracellular regulators, a significant part of which are still unknown, can play an important role.
Using transcriptomic and proteomic analysis, we searched for new regulators of the signal pathway in pea Pisum sativum L., which are activated under the influence of Nod factors. Phospholipases C and D, GTPases, calcium-dependent protein kinases, and mitogen-activated protein kinases (MAPKs) have been identified among such novel regulators. The influence of one of such regulators, the MAPK6, on the development of symbiosis was studied in more detail. Using genetic engineering approaches, we increased the transcriptional activity of MAPK6 in transgenic roots, which led to an increase in the number of nodules and the biomass of pea plants. A similar effect was also found for the homologous MAPK6 gene in another legume Medicago truncatula, which has a type of nodulation similar to that one in pea. New approaches have been developed to obtain stable pea transformants with enhanced level of MAPK6 transcription using the constitutive p35S promoter. A comprehensive study of such plants inoculated with rhizobia was carried out. In addition, the approaches for genome editing of pea plants have been designed using the CRISPR/Cas system, when the MAPK6 gene was used as a target. Thus, the possibility of using genetic engineering methods to obtain plants with increased symbiosis efficiency was investigated.
Об авторах
Elena Dolgikh
All-Russia Research Institute for Agricultural Microbiology
Email: dol2helen@yahoo.com
ORCID iD: 0000-0002-5375-0943
SPIN-код: 4453-2060
Cand. Sci. (Biol.), Leading Researcher, Laboratory of Molecular and Cellular Biology
Россия, Saint PetersburgElizaveta Kantsurova
All-Russia Research Institute for Agricultural Microbiology
Email: rudaya.s.e@gmail.com
ORCID iD: 0000-0002-3081-9880
SPIN-код: 4752-1910
Junior Researcher, Signal Regulation Laboratory
Россия, Saint PetersburgPolina Kozyulina
All-Russia Research Institute for Agricultural Microbiology
Email: polykoz@gmail.com
Senior Researcher, Signal Regulation Laboratory
Россия, Saint PetersburgAndrey Bovin
All-Russia Research Institute for Agricultural Microbiology
Email: andy-piter2007@mail.ru
ORCID iD: 0000-0003-4061-435X
SPIN-код: 8119-0360
Junior Researcher
Россия, Saint PetersburgAlexandra Dolgikh
All-Russia Research Institute for Agricultural Microbiology; Saint Petersburg State University
Email: sqshadol@gmail.com
ORCID iD: 0000-0003-1845-9701
SPIN-код: 2602-1514
Engineer
Россия, Saint Petersburg; Saint PetersburgAlina Dymo
All-Russia Research Institute for Agricultural Microbiology
Email: dymoalina@yandex.ru
SPIN-код: 8378-4048
Junior Researcher
Россия, Saint PetersburgNikolai Kozlov
All-Russia Research Institute for Agricultural Microbiology; Saint Petersburg State University
Автор, ответственный за переписку.
Email: bionkbio@gmail.com
Student, Department of Genetics and Biotechnology
Россия, Saint Petersburg; Saint Petersburg