Using the SSAP analysis for the primary localization of the cdt (cadmium tolerance) mutation in pea linkage group VI



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Abstract

To localize the cdt mutation leading to an increased cadmium tolerance in the pea mutant and an increased cadmium accumulation in the biomass, F2 and F3 progenies from crosses between the mutant SGECdt line and the JI 281 line were analyzed. The joint inheritance of 89 SSAP (sequence specific amplified polymorphism) markers, by which the analyzed lines differed, and the mutant trait of cadmium tolerance was performed. The linkage between the trait of cadmium tolerance and 4 SSAP markers: Tps1/146+, Tps1/167+,Tps1/44+ and Tps1/58+, localized in VI pea linkage group, was shown. Thus, prospects of using SSAP analysis for primary localization of a mutation in the linkage group were demonstrated.

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About the authors

Viktor E Tsyganov

All-Russia Research Institute for Agricultural Microbiology, Saint-Petersburg, RF

Email: viktor_tsyganov@arriam.spb.ru

Olga A Kulaeva

All-Russia Research Institute for Agricultural Microbiology, Saint-Petersburg, RF

Email: koa1983@yandex.ru Podbelskiy chausse, 3, Saint-Petersburg, Pushkin, 196608, Russia

Maggie Knox

 John Innes Centre, Norwich, UK

Email: maggie.knox@bbsrc.ac.uk

Aleksey U Borisov

All-Russia Research Institute for Agricultural Microbiology, Saint-Petersburg, RF

Email: ayborisov@yandex.ru

Igor A Tikhonovich

All-Russia Research Institute for Agricultural Microbiology, Saint-Petersburg, RF

Email: arriam@arriam.spb.ru. contact@arriam.spb.ru Podbelskiy Ch., 3, Saint-Petersburg, Pushkin-8

Tomas N Ellis

Institute of Biological, Environmental and Rural Sciences, Aberystwyth, UK

Email: noe2@aber.ac.uk

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Copyright (c) 2012 Tsyganov V.E., Kulaeva O.A., Knox M., Borisov A.U., Tikhonovich I.A., Ellis T.N.

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