Diversity of the species of genus Avena revealed by morphological characters and resistance to Fusarium infection of grain

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Sixty six genotypes belong to wild and cultivated Avena species from the VIR collection were evaluated for infection of grain by Fusarium fungi and mycotoxins in accumulation. Among genotypes 13.6, 28.8, and 57.6% were diploid, tetraploid, and hexaploid oats, respectively.

The aim of this study was to recognize the interrelationship between the wild species of the genus Avena not subjected to formal breeding programs, and Fusarium fungi, which have been reported as predominant seed borne pathogens. The real-time PCR was used to simultaneously detect and quantify fungi in grain of Avena genotypes. Mycotoxin analysis was performed by ELISA. The average amount of Fusarium DNA and deoxynivalenol (DON) in groups of the tetraploid oats were higher than they were found in the groups of di- and hexaploid species. It was determining the strong correlation between the presence of Tri-Fusarium DNA and DON content (p < 0,01). The low amounts of DON in the grain were detected in seven hexaploid genotypes (A. byzantina, A. sterilis, A. sativa, and A. fatua) and one diploid A. wiestii. The connection of morphological characters (weight of 1000 grains, husk hardness, trichomes profusion and plant height) of Avena species and the indicators of Fusarium infection were analyzed. Only biomass of Fusarium has demonstrated significant connection with weight of 1000 grains. Significant correlation between the hardness of husk and the amounts of Fusarium DNA (p < 0,01) was detected. For Fusarium the strong protective effect of the husk from the penetration of pathogens into grain was evident.

Taiana Yu Gagkaeva

Author for correspondence.
All-Russian Scientific Research Institute of Plant Protection (VIZR)
Russian Federation, Pushkin, Saint Petersburg, Russia

Ph.D., Senior Scientist, Docent in Mycology

Olga P Gavrilova

All-Russian Scientific Research Institute of Plant Protection (VIZR)
Russian Federation, Pushkin, Saint Petersburg, Russia

Ph.D., Researcher

Alexandra A Orina

All-Russian Scientific Research Institute of Plant Protection (VIZR)
Russian Federation, Pushkin, Saint Petersburg, Russia

Ph.D., Researcher

Elena V Blinova

Federal Research Center N.I. Vavilov All-Russian Institute of Plant Genetic Resources (VIR)
Russian Federation, Saint Petersburg, Russia

Ph.D., Researcher

Igor G Loskutov

Federal Research Center N.I. Vavilov All-Russian Institute of Plant Genetic Resources (VIR)
Russian Federation, Saint Petersburg, Russia

Dr. of Biological Sci., Prof., Head of department of Genetic Resources Oat, Barley, Rye (VIR)

  • Loskutov IG. On evolutionary pathways of Avena species. Gen Res Crop Evol. 2007;55:211-220. doi: 10.1007/s10722-007-9229-2.
  • Loskutov IG, Rines HW. Avena L. Wild crop relatives: genomic and breeding resources. In: Kole C, editor. Wild crop relatives: genomic and breeding resources. Cereals. Heidelberg, Berlin, New York: Springer; 2011. P. 109-184. doi: 10.1007/978-3-642-14228-4_3.
  • Loskutov IG, Melnikova SV, Bagmet LV. Eco-geographical assessment of Avena L. wild species at the VIR herbarium and genebank collection. Gen Res Crop Evol. 2017;64:177-188. doi: 10.1007/s10722-015-0344-1.
  • Stenglein SA. Fusarium poae: a pathogen that needs more attention. J Plant Pathol. 2009;91:25-36. doi: 10.4454/jpp.v91i1.621.
  • Гаврилова О.П., Гагкаева Т.Ю., Буркин А.А., Кононенко Г.П. Зараженность грибами рода Fusarium и контаминация микотоксинами зерна овса и ячменя на севере Нечерноземья // Сельскохозяйственная биология. – 2009. – № 6. – C. 89–93. [Gavrilova OP, Gagkaeva TY, Burkin AA, Kononenko GP. Mycological infection by Fusarium strains and mycotoxins contamination of oats and barley in the north of Nonchernozem’e. Agricultural biology. 2009;(6):89-93. (In Russ.)]
  • Гаврилова О.П., Ганнибал Ф.Б., Гагкаева Т.Ю. Зараженность зерна овса грибами Fusarium и Alternaria и ее сортовая специфика в условиях Северо-Запада России // Сельскохозяйственная биология. – 2016. – Т. 51. – № 1. – С. 111–118. [Gavrilova OP, Gannibal PB, Gagkaeva TY. Fusarium and Alternaria fungi in grain of oats grown in the north-western Russia regarding cultivar specificity. Agricultural biology. 2016;1:111-118. (In Russ.)]. doi: 10.15389/agrobiology.2016.1.111rus.
  • Thrane U, Adler A, Clasen P, et al. Diversity in metabolite production by Fusarium langsethiae, Fusarium poae and Fusarium sporotrichioides. Int J Food Microbiol. 2004;95:257-266. doi: 10.1016/j.ijfoodmicro.2003.12.005.
  • Boutigny A-L, Richard-Forget F, Barreau C. Natural mechanisms for cereal resistance to the accumulation of Fusarium trichothecenes. Eur J Plant Pathol. 2008;121: 411-423. doi: 10.1007/s10658-007-9266-x.
  • 10th International Oat Conference; 2016 Jul 11-15; Saint Petersburg, Russia. Available at: http://oats2016.org/ http://www.oats2016.org/files/29614_BLOK_farexpo_tezisy_kongress.pdf.
  • Bjørnstad Ǻ, Skinnes H. Resistance to Fusarium infection in oats (Avena sativa L.). Cereal Res Commun. 2008;36:57-61. doi: 10.1556/CRC.36.2008.Suppl.B.9.
  • Germeier C, Maggioni L, Katsiotis A, Lipman E. Report of a working group on Avena. In: Sixth meeting “Avena genetic resources for quality in human consumption” (AVEQ); 2010 Oct 19-22; Bucharest, Romania; 2010. P. 1-35.
  • Лоскутов И.Г., Блинова Е.В., Гаврилова О.П., Гагкаева Т.Ю. Разнообразие культурного овса по хозяйственно ценным признакам и их связь с устойчивостью к фузариозу // Вавиловский журнал генетики и селекции. – 2016. – Т. 20. – № 3. – С. 286–294. [Loskutov IG, Blinova EV, Gavrilova OP, Gagkaeva TY. The valuable characteristics of oats genotypes and resistance to Fusarium disease. Russian journal of genetics: applied research. 2016;20(3):286-294. (In Russ.)]. doi: 10.18699/VJ16.151.
  • Лоскутов И.Г., Ковалева О.Н., Блинова Е.В. Методические указания по изучению и сохранению мировой коллекции ячменя и овса. – СПб.: ВИР, 2012. [Loskutov IG, Kovaleva ON, Blinova EV. Methodological guidance directory for studing and maintaining VIR’s collections of barley and oat. Saint Petersburg: VIR; 2012. (In Russ.)]
  • European Commission. Community reference laboratory for GM food and feed. Event-specific for the quantitation of maize line NK603 using real-time PCR. 2005. Available at: http://gmo-crl.jrc.ec.europa.eu/summaries/NK603report_mm.pdf.
  • Gagkaeva TY, Gavrilova OP, Loskutov IG, Yli-Mattila T. Sources of resistance to Fusarium head blight in VIR oat collection. Euphytica. 2013;191:355-364. doi: 10.1007/s10681-013-0865-7.
  • Halstensen AS, Nordby KC, Eduard W, Klemsdal SS. Real-time PCR detection of toxigenic Fusarium in airborne and settled grain dust and associations with trichothecene mycotoxins. J Environm Monitoring. 2006;8:1235-1241. doi: 10.1039/b609840a.
  • Yli-Mattila T, Paavanen-Huhtala S, Jestoi M, et al. Real-time PCR detection and quantification of Fusarium poae, F. graminearum, F. sporotrichioides and F. langsethiae in cereal grains in Finland and Russia. Arc Phytopathol Plant Protect. 2008;41(4):243-260. doi: 10.1080/03235400600680659.
  • Konstantinova P, Yli-Mattila T. IGS-RFLP analysis and development of molecular markers for identification of F. poae, F. langsethiae, F. sporotrichioides and F. kyushuense. Int J Food Microbiol. 2004;95:321-331.
  • Loskutov IG, Kosareva IA, Melnikova SV, et al. Genetic diversity in tolerance of wild Avena species to aluminium (Al) Gen Res Crop Evol. 2016. doi: 10.1007/s10722-016-0417-9.
  • Banu Buyukunal Bal E. Efficacy of seed storage proteins of cereal grains on Aspergillus and Fusarium spp. Animal Health Prod Hyg. 2012;1:47-51.
  • Kazemi HA, Shimi P. Determination of the host range of Fusarium moniliforme isolated from winter wild oat (Avena ludoviciana) in Iran. Iranian Journal of Weed Science. 2005;1(1):67-72.
  • Liang C, Qing-Yun G. Potential research of Fusarium Avenaceum isolate GD-2 as a bioherbicide agent for wild oats (Avena fatua L.). J Agric Sci Technol. 2014;16(3):70.
  • de Luna LZ, Kennedy AC, Hansen JC, et al. Mycobiota on wild oat (Avena fatua L.) seed and their caryopsis decay potential. Plant Health Prog. 2011. doi: 10.1094/PHP-2011-0210-01-RS.
  • Левитин М.М., Иващенко В.Г., Шипилова Н.П., и др. Возбудители фузариоза колоса зерновых культур и форм проявления болезни на Северо-Западе России // Микология и фитопатология. – 1994. – Т. 28. – № 3. – С. 58–64. [Levitin MM, Ivashchenko VG, Shipilova NP, et al. Fusarium head blight pathogens and manifestation forms of the disease in North-West Russia. Mycology and phytopathology. 1994;28(3):58-64. (In Russ.)]
  • Bourdages JV, Marchand S, Rioux S, Belzile FJ. Diversity and prevalence of Fusarium species from Quebec barley fields. Can J Plant Pathol. 2006;28:419-425. doi: 10.1080/07060660609507315.
  • Гагкаева Т.Ю., Шамшев И.В., Гаврилова О.П., Селицкая О.Г. Биология взаимоотношений грибов рода Fusarium и насекомых (обзор) // Сельскохозяйственная биология. – 2014. – № 3. – С. 13–23. [Gagkaeva TY, Shamshev IV, Gavrilova OP, Selitskaya OG. Biological relationships between Fusarium fungi and insects (review). Agricultural biology. 2014;(3):13-23. (In Russ.)]. doi: 10.15389/agrobiology.2014.3.13rus.
  • Степанычева Е.А., Петрова М.О., Черменская Т.Д., и др. Эколого-биохимические взаимодействия грибов рода Fusarium и фитофагов злаковых культур // Евразиатский энтомологический журнал. – 2016. – Т. 15. – № 6. – С. 530–537. [Stepanycheva EA, Petrova MO, Chermenskaya TD, et al. Ekologo-biokhimicheskie vzaimodeistviya gribov roda Fusarium i fitofagov zlakovykh kul’tur. Euroasian entomological journal. 2016;15(6):530-537. (In Russ.)]
  • Savelieva E, Gustyleva L, Migalovskaya E, et al. Study of the vapor phase over Fusarium fungi cultured on various substrates. Chem Biodivers. 2016;13(7):891-903. doi: 10.1002/cbdv.201500284.

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Copyright (c) 2017 Gagkaeva T.Y., Gavrilova O.P., Orina A.A., Blinova E.V., Loskutov I.G.

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