The role of WNT and HOXA signaling cascades in the pathogenesis of adenomyosis
- 作者: Malysheva O.V.1, Beganova A.K.1, Vashukova E.S.1, Shalina M.A.1, Yarmolinskaya M.I.1,2, Glotov A.S.1
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隶属关系:
- The Research Institute of Obstetrics, Gynecology and Reproductology named after D.O. Ott
- North-Western State Medical University named after I.I. Mechnikov
- 期: 卷 72, 编号 1 (2023)
- 页面: 27-37
- 栏目: Original Research
- ##submission.dateSubmitted##: 24.01.2023
- ##submission.dateAccepted##: 25.01.2023
- ##submission.datePublished##: 29.03.2023
- URL: https://journals.eco-vector.com/jowd/article/view/121803
- DOI: https://doi.org/10.17816/JOWD121803
- ID: 121803
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BACKGROUND: Adenomyosis is a common gynecological disease with unknown pathogenesis. The HOXA10, HOXA11 and WNT4 genes may play an important role in the pathogenesis of adenomyosis both at the stage of embryonic development and in the postnatal period. The study of their expression in the endometrium of patients with adenomyosis can expand the understanding of the pathogenesis of this disease.
AIM: The aim of this work was to study the peculiarity of the WNT4, HOXA10 and HOXA11 gene expression in the eutopic endometrium of patients with isolated adenomyosis.
MATERIALS AND METHODS: The study included 38 women: the main group involved patients with isolated adenomyosis established by ultrasound / magnetic resonance imaging (n = 20) and the control group consisted of healthy patients (n = 18). Endometrial sampling was obtained during surgery or by aspiration biopsy at 5–12 day of the menstrual cycle (proliferative phase) or 20–24 day of the menstrual cycle (secretory phase). The expression of the WNT4, HOXA10 and HOXA11 genes in endometrial samples was assessed by a real-time reverse transcription polymerase chain reaction.
RESULTS: In the proliferative phase endometrial samples of patients with adenomyosis, a significant increase in the WNT4 (of almost two times), HOXA10 and HOXA11 (of one and a half to two times) gene expression levels was shown compared to the control group. In 88% of patients with adenomyosis, there is a significant increase (up to the level of fourth quartile) in the expression of at least one of these genes, such changes being not typical for the endometrium of women in the control group. In the secretory phase endometrial samples, the expression of the studied genes did not differ from the level characteristic of the corresponding groups in the proliferative phase of the cycle.
CONCLUSIONS: The aberrant expression of the WNT4, HOXA10 and HOXA11 genes in the endometrium of patients with adenomyosis indicates a significant role of these genes in the development of the disease and infertility associated with adenomyosis.
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作者简介
Olga Malysheva
The Research Institute of Obstetrics, Gynecology and Reproductology named after D.O. Ott
编辑信件的主要联系方式.
Email: omal99@mail.ru
ORCID iD: 0000-0002-8626-5071
SPIN 代码: 1740-2691
Scopus 作者 ID: 6603763549
Researcher ID: O-9897-2014
Cand. Sci. (Biol.)
俄罗斯联邦, Saint PetersburgAlexandra Beganova
The Research Institute of Obstetrics, Gynecology and Reproductology named after D.O. Ott
Email: alexandra.beganova@yandex.ru
ORCID iD: 0000-0002-4705-7990
SPIN 代码: 2612-9889
俄罗斯联邦, Saint Petersburg
Elena Vashukova
The Research Institute of Obstetrics, Gynecology and Reproductology named after D.O. Ott
Email: vi_lena@list.ru
ORCID iD: 0000-0002-6996-8891
SPIN 代码: 2811-8730
Researcher ID: D-3422-2017
Cand. Sci. (Biol.)
俄罗斯联邦, Saint PetersburgMaria Shalina
The Research Institute of Obstetrics, Gynecology and Reproductology named after D.O. Ott
Email: amarus@inbox.ru
ORCID iD: 0000-0002-5921-3217
SPIN 代码: 6673-2660
Researcher ID: A-7180-2019
MD, Cand. Sci. (Med.)
俄罗斯联邦, Saint PetersburgMaria Yarmolinskaya
The Research Institute of Obstetrics, Gynecology and Reproductology named after D.O. Ott; North-Western State Medical University named after I.I. Mechnikov
Email: m.yarmolinskaya@gmail.com
ORCID iD: 0000-0002-6551-4147
SPIN 代码: 3686-3605
Scopus 作者 ID: 7801562649
Researcher ID: P-2183-2014
MD, Dr. Sci. (Med.), Professor, Professor of the Russian Academy of Sciences
俄罗斯联邦, Saint Petersburg; Saint PetersburgAndrey Glotov
The Research Institute of Obstetrics, Gynecology and Reproductology named after D.O. Ott
Email: anglotov@mail.ru
ORCID iD: 0000-0002-7465-4504
SPIN 代码: 1406-0090
Scopus 作者 ID: 7004340255
Researcher ID: E-8525-2015
Dr. Sci. (Biol.)
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