Role of lipid metabolism and systemic inflammation in the development of atherosclerosis in animal models

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Abstract

Systemic inflammation makes a significant contribution to the pathogenesis of atherosclerosis and has been the subject of numerous studies. Works aiming to analyze the mechanisms of atherosclerosis development often include experiments on animals. A primary task of such research is the characterization, justification, and selection of an adequate model.

Aim. To evaluate the peculiarities of lipid metabolism and systemic inflammation in chronic obstructive pulmonary disease (COPD) in the development of atherosclerosis in animal models.

Materials and Methods. Analyses of cross-links between species-specific peculiarities of lipid metabolism and the immune response, as well as a bioinformatic analysis of differences in Toll-like receptor 4 (TLR4) in mice, rats, and rabbits in comparison with its human homolog, were carried out. A search for and analysis of the amino acid sequences of human, mouse, rat, and rabbit TLR4 was performed in the International database GenBank of National Center of Biotechnical Information and in The Universal Protein Resource (UniProt) database. Multiple alignments of the TLR4 amino acid sequences were implemented in the Clustal Omega program, version 1.2.4. Reconstruction and visualization of molecular phylogenetic trees were performed using the MEGA7 program according to the Neighbor-Joining and Maximum Parsimony methods.

Results. Species-specific differences of the peculiarities of lipid metabolism and the innate immune response in humans, mice, and rabbits were shown that must be taken into account in analyses of study results.

Conclusion.Disorders in lipid metabolism and systemic inflammation mediated by the innate immune system participating in the pathogenesis of atherosclerosis in COPD possess species-specific differences that should be taken into account in analyses of study results.

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About the authors

Stanislav N. Kotlyarov

Ryazan State Medical University

Author for correspondence.
Email: SKMR1@yandex.ru
ORCID iD: 0000-0002-7083-2692
SPIN-code: 3341-9391
ResearcherId: Q-3633-2017

MD, PhD, Head of the Nurse Department

Russian Federation, Ryazan, Russia

Anna A. Kotlyarova

Ryazan State Medical University

Email: kaa.rz@yandex.ru
SPIN-code: 9353-0139
ResearcherId: K-7882-2018

PhD in Biological Sciences, Assistant of the Department of Pharmacology with the Course of Pharmacy of the Faculty of Additional Professional Education

Russian Federation, Ryazan, Russia

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Supplementary files

Supplementary Files
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1. Fig. 1. Alignment of amino acid sequences of human, mouse, rat, and rabbit TLR4 (TIR domain-containing protein). Implemented in the CLUSTAL O program, version 1.2.4. The table of identity of amino acid sequences was constructed using the BLAST® tool (Basic Local Alignment Search Tool)

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2. Fig. 2. Phylogenetic tree of TLR4 of humans, mice, rats, and rabbits (protein-containing TIR-domain) constructed using the Neighbor-Joining method

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Copyright (c) 2021 Kotlyarov S., Kotlyarova A.A.



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