Features of profiling methods for the expression of long non-coding RNAs in tumors

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Abstract


With rapid advances in high-performance genome analysis technology, studying of long non-coding RNAs (lncRNAs) has become a very popular topic in biomedical research. LncRNAs are a group of non-coding RNAs with a length of more than 200 nucleotides. LncRNAs play a fundamental role in cell proliferation and differentiation, and epigenetic regulation. Thus, studying lncRNAs will give a new understanding of gene regulation and will open up new possibilities for treating and diagnosing many diseases, including tumors. The identification of new molecular markers in the form of circulating lncRNAs will improve tumor detection, predicting the course of the disease, treatment planning, and diagnosing at the earliest signs of upcoming neoplastic transformation. Treatment of tumors, especially malignant ones, is also a difficult task. When surgery and chemotherapy are not effective, radiation therapy becomes the treatment of choice. Therefore, the possibility of lncRNAs being innovative therapeutic agents in tumors is a viable idea. However, the possibility of their use in modern clinical practice is limited due to the number of problems associated with significant differences between procedures for processing samples, methods of analysis, and especially strategies for standardizing results. Another problem for conducting studies on the profiling of lncRNAs expression is their large number and the diversity of their functions in tumors. Therefore, the solution of technological problems on the profiling of lncRNAs expression in tumors may open up new possibilities for the use of lncRNAs in modern clinical practice.


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About the authors

Ozal Arzuman A. Beylerli

Bashkir State Medical University of the Ministry of Healthcare of the Russian Federation

Author for correspondence.
Email: obeylerli@mail.ru
ORCID iD: 0000-0002-6149-5460

Russian Federation, Rep. Bashkortostan, Ufa

MD, postgraduate student of the Department of Urology with the course IPDE

Ilgiz F. Gareev

Bashkir State Medical University of the Ministry of Healthcare of the Russian Federation

Email: ilgiz_gareev@mail.ru
ORCID iD: 0000-0002-4965-0835

Russian Federation, Rep. Bashkortostan, Ufa

научный сотрудник Центральной научно-исследовательской лаборатории

Adel Izmailov

Regional Clinical Oncology Center

Email: izmailov75@mail.ru

Russian Federation, Rep. Bashkortostan, Ufa

Главный врач

Oleg N. Lipatov

Bashkir State Medical University of the Ministry of Healthcare of the Russian Federation

Email: lipatovoleg@bk.ru

Russian Federation, Rep. Bashkortostan, Ufa

профессор кафедры Онкологии с курсами онкологии и патологической анатомии ИДПО

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Copyright (c) 2020 Beylerli O.A., Gareev I.F., Izmailov A., Lipatov O.N.

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