GENETIC VARIABILITY AND POPULATION DIFFERENTIATION OF OPISTHORCHIS FELINEUS (TREMATODA) FROM WEST SIBERIA RIVERS



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Abstract

Genetic variability in Opisthorchis felineus from 6 Western Siberia rivers was studied using allozyme and multilocus DNA markers. Genetic subdivision of populations and the spatial genetic variability in O. felineus maritas were found to be the same when using two methods of analysis, but differed from these indices in metacercariae. Metacercariae from ide, dace and roach were not genetically different, indicating the absence of hostal subpopulations of the parasite. Low level of O. felineus genetic variability is not consistent with a pronounced population structure of intermediate hosts — Cyprinid fish.

About the authors

Oksana N Zhigileva

Tyumen State University, Tyumen, Tyumen Oblast, RF

Email: zhigileva@mail.ru Pirogova St., 3, Tyumen, 625043

Daria V Zenovkina

Tyumen State University, Tyumen, Tyumen Oblast, RF

Email: agricola313@gmail.com

Tatyana A Zamyatina

Tyumen State University, Tyumen, Tyumen Oblast, RF

Email: uslamina.tatyana@mail.ru Pirogova St., 3, Tyumen, 625043, Russia

References

  1. Беэр С. А., 2005. Биология возбудителя описторхоза. М: Товарищество научных изданий КМК. 336 с.no socket
  2. Катохин А. В., Шеховцов С. В., Конков С. и др., 2008. Оценка генетических отличий Opisthorchis felineus от Opisthorchis viverrini и Clonorchis sinensis по ITS2-и CO1-последовательностям // Доклады академии наук. Т. 421, № 4. С. 549–552.no socket
  3. Корочкин Л. И., Серов О. А., Пудовкин А. И. и др., 1977. Генетика изоферментов. М.: Наука. 278 с.no socket
  4. Лоскутова З. Ф., 1980. Виварий. М.: Медицина. 96 с.no socket
  5. Маурер Г., 1971. Диск-электрофорез. Теория и практика электрофореза в ПААГ. М.: Мир. 243 с.no socket
  6. Поляков А. В., Катохин А. В., Бочарова Т. А. и др., 2010. Сравнительный анализ кариотипов Opisthorchis felineus из Западной Сибири // Сибирский экологический журнал. Т. 17, № 1. С. 3–6.no socket
  7. Степанова Т. Ф., 2002. Описторхоз. Тюмень: издательство ТюмГУ. 196 с.no socket
  8. Экология рыб Обь-Иртышского бассейна, 2006 / под ред. Д. С. Павлова, А. Д. Мочека. М.: Товарищество научных изданий КМК. 596 с.no socket
  9. Andrews R. H., Sithithaworn P., Petney T. N., 2008. Opisthorchis viverrini: an underestimated parasite in world health // Trends Parasitol. Vol. 24, N 11. P. 497–501.no socket
  10. Bender W., Pierre S., Hognes D. S., 1983. Chromosomal walking and jumping to isolate DNA from Ace and rosy loci of bithorax complex in Drosophila melanogaster // Journal Mol. Biol. Vol. 168. P. 17–33.no socket
  11. Gandon S., Michalakis Y., 2002. Local adaptation, evolutionary potential and host-parasite coevolution: interactions between migration, mutation, population size and generation time // Journal of Evolutionary Biology. Vol. 15. P. 451–462.no socket
  12. King S., Scholz T., 2001. Trematodes of the family Opisthorchiidae: a minireview // Korean J. Parasitol. Vol. 39(3). P. 209–221.no socket
  13. Nei M., 1973. Analysis of gene diversity in subdivided populations // Proc. Natl. Acad. Sci. USA. Vol. 70. P. 3321–3323.no socket
  14. Nei M., 1978. Estimation of average heterozygosity and genetic distance from a small number of individuals // Genetics. Vol. 89. P. 583–590.no socket
  15. Peacock A. C., Dingman C. W., 1967. Resolution of multiple ribonucleic acid species by polyacrylamide gel electrophoresis // Biochemistry. Vol. 6. P. 1818–1827.no socket
  16. Petkeviciūte R., Stunzenas V., Staneviciūte G., Sokolov S. G., 2010. Comparison of the developmental stages of some European allocreadiid trematode species and a clarification of their life cycles based on ITS2 and 28S sequences // Syst Parasitol. Vol. 76, N 3. P. 169–178.no socket
  17. Rannala B., 1991. Evidence for host allozymes on electrophoretic gels of trematode parasites (Digenea, Plagiorchiiformes) // Journal of Parasitology. Vol. 77. P. 805–808.no socket
  18. Richardson B. J., 1986. Allozyme electrophoresis. A handbook for animal systematics and Population Studies. London: Academie Press. 410 pp. Saijuntha W., Sithithaworn P., Wongkham S. et al., 2006.no socket
  19. Enzyme markers to identify and characterize Opisthorchis viverrini in Thailand and Laos // Southeast Asian J. Trop. Med. Public Health. Vol. 37 (Suppl. 3). P. 43–47.no socket
  20. Saijuntha W., Sithithaworn P., Wongkham S. et al., 2007. Evidence of a species complex within the food-borne trematode Opisthorchis viverrini and possible co-evolution with their first intermediate hosts // Int. J. Parasitol. Vol. 37. P. 695–703.no socket
  21. Saijuntha W., Sithithaworn P., Wongkham S. et al., 2008. Genetic variation at three enzyme loci within a Thailand population of Opisthorchis viverrini // Parasitol. Res. Vol. 103. P. 1283–1287.no socket
  22. Shekhovtsov S. V., Katokhin A. V., Romanov K. V. et al., 2009. A novel nuclear marker, Pm-int9, for phylogenetic studies of Opisthorchis felineus, Opisthorchis viverrini and Clonorchis sinensis (Opisthorchidae, Trematoda) // Parasitol. Res. Vol. 106. P. 293–297.no socket
  23. Shekhovtsov S. V., Katokhin A. V., Kolchanov N. A., Mordvinov V. A., 2010. The complete mitochondrial genomes of the liver flukes Opisthorchis felineus and Clonorchis sinensis (Trematoda) // Parasitology International. Vol. 59, N 1. P. 100–103.no socket
  24. Sithithaworn P., Nuchjungreed C., Srisawangwong T. et al., 2007. Genetic variation in Opisthorchis viverrini (Trematoda: Opisthorchiidae) from northeast Thailand and Laos PDR based on random amplified polymorphic DNA analyses // Parasitol. Res. Vol. 100(3). P. 613–617.no socket
  25. Williams J. G. K., Kubelik A. R., Livak K. J. et al., 1990. DNA polymorphism amplified by arbitrary primers are useful as genetic markers // Nucl. Acids Res. Vol. 18. P. 6531–6535.no socket
  26. Yeh F. C., Yang R., Boyle T., 1999. POPGENE. Version 1.31. University of Alberta and Centre for International Forestry Research. Zhigileva O. N., Ozhirel’ev V. V., Brol I. S., Pozhidaev V. V., 2010.no socket
  27. Zhigileva O. N., Ozhirel’ev V. V., Brol I. S., Pozhidaev V. V., 2010. Population structure of three fish species (Cypriniformes: Cyprinidae) living in rivers of the Ob-Irtysh basin, by the data of isoenzyme analysis // J. of Ichthyology. Vol. 50, N 9. P. 778–787.no socket
  28. Zietjiewicz E., Rafalski A., Labuda D., 1994. Genome fingerprinting by simple sequence repeat (SSR)-anchored polymerase chain reaction amplification // Genomics. Vol. 20. P. 176–183.no socket

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Copyright (c) 2012 Zhigileva O.N., Zenovkina D.V., Zamyatina T.A.

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