The evolution of ideas on the biological role of 5-methylcytosine oxidative derivatives in the mammalian genome

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Abstract


Summary: In this review, we summarize data on 5-hydroxymethylcytosine, 5-formylcytosine and 5-carboxylcytosine – cytosine modifications which are produced by TET-mediated oxidation of 5-methylcytosine in DNA. We show the biochemistry of modified cytosine as well as methods for its global and location analysis. We also highlight the milestones in the evolution of ideas on the biological role of 5-hydroxymethylcytosine, 5-formylcytosine and 5-carboxylcytosine in the mammalian genome since their discovery in 2009 till present.


Olga A Efimova

Author for correspondence.
efimova_o82@mail.ru
D.O. Ott Research Institute of Obstetrics, Gynecology and Reproductology
Russian Federation

Researcher, PhD, Laboratory for Prenatal Diagnosis of human inherited and inborn disorders

Anna A Pendina

pendina@mail.ru
D.O. Ott Research Institute of Obstetrics, Gynecology and Reproductology
Russian Federation

Researcher, PhD, Laboratory for Prenatal Diagnosis of human inherited and inborn disorders

Andrei V Tikhonov

tixonov5790@gmail.com
D.O. Ott Research Institute of Obstetrics, Gynecology and Reproductology
Russian Federation

assistant researcher, Laboratory for Prenatal Diagnosis of human inherited and inborn disorders

Vladislav S Baranov

baranov@vb2475.spb.edu
D.O. Ott Research Institute of Obstetrics, Gynecology and Reproductology
Russian Federation

Head of the laboratory, prof, Laboratory for Prenatal Diagnosis of human inherited and inborn disorders

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Supplementary files

Supplementary Files Action
1. Fig. 1. Cycle of cytosine modifications in mammalian cell DNA. С – cytosine, 5mC – 5-methylcytosine, 5hmC – 5-hydroxymethylcytosine, 5fC – 5-formylcytosine, 5caC – 5-carboxylcytosine. View (204KB) Indexing metadata

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