Peculiarities of placental microRNA expression in pregnancies complicated by gestational diabetes mellitus and preeclampsia

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Abstract


The aim of study was to determine feasible changes of placental miRNAs expression profiles revealed by next generation sequencing (NGS) in pregnancies with GDM complicated or not with PE. Out of 27 miRNAs, studied expression was significantly different (FDR < 0.05) only for his-miR-45a. Comparative analysis of revealed reliable differences in expression of hsa-miR-4532 (p < 0.0001, FDR = 0.0008), hsa-miR-34c-5p (p < 0.0001, FDR = 0.0083), and hsa-miR-193b-5p (p < 0.0001, FDR = 0.0139) in pregnancy complicated by PE, without of GDM. The present results suggest that GDM and PE are associated with specific alterations in the placental miRNA expression profiles. Further studies are needed to verify the role of these microRNA in molecular mechanisms underlying GDM and PE pathogenesis.


Vladimir S. Pakin

Author for correspondence.
pakins@list.ru
FSBSI “The Research Institute of Obstetrics, Gynecology and Reproductology named after D.O. Ott”
Russian Federation, 3, Mendeleevskaya line, Saint Petersburg, 199034

PhD student

Elena S. Vashukova

vi_lena@list.ru
FSBSI “The Research Institute of Obstetrics, Gynecology and Reproductology named after D.O. Ott”
Russian Federation, 3, Mendeleevskaya line, Saint Petersburg, 199034

scientist

Roman V. Kapustin

kapustin.roman@gmail.com
FSBSI “The Research Institute of Obstetrics, Gynecology and Reproductology named after D.O. Ott”
Russian Federation, 3, Mendeleevskaya line, Saint Petersburg, 199034

PhD

Olga N. Arzhanova

arjanova_olga@mail.ru
FSBSI “The Research Institute of Obstetrics, Gynecology and Reproductology named after D.O. Ott”
Russian Federation, 3, Mendeleevskaya line, Saint Petersburg, 199034

head of the Department Pathology of pregnancy, professor

Andrey S. Glotov

anglotov@mail.ru
FSBSI “The Research Institute of Obstetrics, Gynecology and Reproductology named after D.O. Ott”
Russian Federation, 3, Mendeleevskaya line, Saint Petersburg, 199034

senior researcher of the Laboratory prenatal diagnosis of hereditary and congenital diseases

Vladislav S. Baranov

baranov@vb2475.spb.ed
FSBSI “The Research Institute of Obstetrics, Gynecology and Reproductology named after D.O. Ott”
Russian Federation, 3, Mendeleevskaya line, Saint Petersburg, 199034

head of the Laboratory prenatal diagnosis of hereditary and congenital diseases, Corr. RAS, Professor

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