New rhizosphere herbicide-degrading strains capable of biocontrol of phytopathogenic microorganisms

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Abstract

Biotechnologically significant strains of bacteria of the Pseudomonas genus have been isolated from the rhizosphere of wild and cultivated cereals. The isolated strains are able to biocontrol the phytopathogenic fungi and bacteria and degrade the herbicide glyphosate as the only source of phosphorus (P. chlororaphis subsp. chlororaphis G16, P. chlororaphis subsp. aureofaciens G27, P. protegens G23-4) and the herbicide Axial as the only carbon source (P. chlororaphis G27). These strains exhibit lipolytic and proteolytic activities and P. chlororaphis G27 can also produce hydrogen cyanide, which has an antifungal effect. The studied strains can be useful as the basis of biopreparations for protecting plants from phytopathogenic microorganisms and treating soils polluted with herbicides.

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T. Z. Esikova

G.K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Pushchino Scientific Center for Biological Research of the Russian Academy of Sciences

Author for correspondence.
Email: das3534@rambler.ru
Russian Federation, Pushchino, Moscow Region, 142290

V. N. Polivtseva

G.K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Pushchino Scientific Center for Biological Research of the Russian Academy of Sciences

Email: das3534@rambler.ru
Russian Federation, Pushchino, Moscow Region, 142290

T. O. Anokhina

G.K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Pushchino Scientific Center for Biological Research of the Russian Academy of Sciences

Email: das3534@rambler.ru
Russian Federation, Pushchino, Moscow Region, 142290

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2. Fig. 1. Antagonistic activity of selected rhizosphere strains against phytopathogenic fungi Fusarium graminearum VKM F-1668 and Cladosporium sphaerospermum T34. The control strain P. chlororaphis BS1393, a producer of phenazine antibiotics, and the studied strains G16, G23-4, G27 were applied to the dish in three replicates.

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3. Fig. 2. Growth dynamics of rhizosphere antagonist strains in a liquid mineral medium: a ‒ with glyphosate as the only source of phosphorus; b ‒ with Axial as the only source of carbon and energy. 1 – strain G27, 2 – G23-4, 3 – G16.

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