New rhizosphere herbicide-degrading strains capable of biocontrol of phytopathogenic microorganisms

封面

如何引用文章

全文:

开放存取 开放存取
受限制的访问 ##reader.subscriptionAccessGranted##
受限制的访问 订阅存取

详细

Biotechnologically significant strains of bacteria of the Pseudomonas genus have been isolated from the rhizosphere of wild and cultivated cereals. The isolated strains are able to biocontrol the phytopathogenic fungi and bacteria and degrade the herbicide glyphosate as the only source of phosphorus (P. chlororaphis subsp. chlororaphis G16, P. chlororaphis subsp. aureofaciens G27, P. protegens G23-4) and the herbicide Axial as the only carbon source (P. chlororaphis G27). These strains exhibit lipolytic and proteolytic activities and P. chlororaphis G27 can also produce hydrogen cyanide, which has an antifungal effect. The studied strains can be useful as the basis of biopreparations for protecting plants from phytopathogenic microorganisms and treating soils polluted with herbicides.

全文:

受限制的访问

作者简介

T. Esikova

G.K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Pushchino Scientific Center for Biological Research of the Russian Academy of Sciences

编辑信件的主要联系方式.
Email: das3534@rambler.ru
俄罗斯联邦, Pushchino, Moscow Region, 142290

V. Polivtseva

G.K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Pushchino Scientific Center for Biological Research of the Russian Academy of Sciences

Email: das3534@rambler.ru
俄罗斯联邦, Pushchino, Moscow Region, 142290

T. Anokhina

G.K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Pushchino Scientific Center for Biological Research of the Russian Academy of Sciences

Email: das3534@rambler.ru
俄罗斯联邦, Pushchino, Moscow Region, 142290

参考

  1. Методы общей бактериологии. Том 3. Под ред. Герхардта Ф. и др. М.: Мир, 1984. 243 с.
  2. Esikova T. Z., Anokhina T. O., Abashina T. N., Suzina N. E., Solyanikova I. P. Characterization of soil bacteria with potential to degrade benzoate and antagonistic to fungal and bacterial phytopathogens // Microorganisms. 2021. V. 9. Art. 755.
  3. Feigl F., Anger V. Replacement of benzidine by copper ethylacetoacetate and tetra base as spot-test reagent for hydrogen cyanide and cyanogen // Analyst (London). 1966. V. 1081. P. 282–284.
  4. Mansfield J., Genin S., Magori S., Citovsky V., Sriariyanum M., Ronald P., Dow M., Verdier V., Beer S. V., Machado M. A., Toth I., Salmond G., Foster G. D. Top 10 plant pathogenic bacteria in molecular plant pathology // Mol. Plant Pathol. 2012. V. 13. P. 614–629.
  5. Mishra J., Arora N. K. Secondary metabolites of fluorescent pseudomonads in biocontrol of phytopathogens for sustainable agriculture // Appl. Soil Ecol. 2018. V. 125. P. 35–45.
  6. Raio A., Puopolo G. Pseudomonas chlororaphis metabolites as biocontrol promoters of plant health and improved crop yield // World J. Microbiol. Biotechnol. 2021. V. 37. Art. 99.
  7. Ramette A., Frapolli M., Fischer-Le Saux M., Gruffaz C., Meyer J. M., Défago G., Sutra L., Moënne-Loccoz Y. Pseudomonas protegens sp. nov., widespread plant-protecting bacteria producing the biocontrol compounds 2,4-diacetylphloroglucinol and pyoluteorin // Syst. Appl. Microbiol. 2011. V. 34. P. 180–188.
  8. Ravensberg W. J. Commercialization of microbes: Present situation and future prospects // Principles of plant-microbe interactions: microbes for sustainable agriculture / Ed. Ben Lugtenberg. Cham: Springer, 2015. Part VI. Ch. 32. P. 309–318.
  9. Sambrook J., Fritsch E. F., Maniatis T. Molecular cloning: a laboratory manual. 2nd ed. N.Y.: Cold Spring Harbor Laboratory Press, 1989. 479 p.
  10. Shahid M., Ahmed B., Khan M. S. Evaluation of microbiological management strategy of herbicide toxicity to green gram plants // Biocatal. Agric. Biotechnol. 2018. V. 14. P. 96–108.
  11. Sidorova T. M., Tomashevich N. S., Allahverdyan V. V., Tupertsev B. S., Kostyukevich Y. I., Asaturova A. M. New Pseudomonas bacterial strains: biological activity and characteristic properties of metabolites // Microorganisms. 2023. V. 11. Art. 1943.
  12. Sviridov A. V., Shushkova T. V., Epiktetov D. O., Tarlachkov S. V., Ermakova I. T., Leontievsky A. A. Biodegradation of organophosphorus pollutants by soil bacteria: biochemical aspects and unsolved problems // Appl. Biochem. Microbiol. 2021. V. 57. P. 836–844.
  13. Veliz E. A., Martínez-Hidalgo P., Hirsch A. M. Chitinase-producing bacteria and their role in biocontrol // AIMS Microbiol. 2017. V. 3. P. 689–705.
  14. Zdor R. E. Bacterial cyanogenesis: impact on biotic interactions // J. Appl. Microbiol. 2015. V. 118. P. 267–274.
  15. Zhumakayev A. R., Vörös M., Szekeres A., Rakk D., Vágvölgyi C., Szűcs A., Kredics L., Škrbić B. D., Hatvani L. Comprehensive characterization of stress tolerant bacteria with plant growth-promoting potential isolated from glyphosate-treated environment // World J. Microbiol. Biotechnol. 2021. V. 37. Art. 94.

补充文件

附件文件
动作
1. JATS XML
下载
2. Fig. 1. Antagonistic activity of selected rhizosphere strains against phytopathogenic fungi Fusarium graminearum VKM F-1668 and Cladosporium sphaerospermum T34. The control strain P. chlororaphis BS1393, a producer of phenazine antibiotics, and the studied strains G16, G23-4, G27 were applied to the dish in three replicates.

下载 (404KB)
索引源数据
3. Fig. 2. Growth dynamics of rhizosphere antagonist strains in a liquid mineral medium: a ‒ with glyphosate as the only source of phosphorus; b ‒ with Axial as the only source of carbon and energy. 1 – strain G27, 2 – G23-4, 3 – G16.

下载 (144KB)
索引源数据

版权所有 © Russian Academy of Sciences, 2025