Role of microorganisms and viruses in the vertical flux in the East Siberian Sea and Laptev Sea

Мұқаба

Дәйексөз келтіру

Толық мәтін

Ашық рұқсат Ашық рұқсат
Рұқсат жабық Рұқсат берілді
Рұқсат жабық Рұқсат ақылы немесе тек жазылушылар үшін

Аннотация

The study of the contribution of bacteria (BAC), heterotrophic nanoflagellates (HNF) and viruses (VIR) to vertical matter fluxes on the shelves of the East Siberian Sea (ESS) and the Laptev Sea (LS) was carried out using sediment traps placed on buoy stations at depths of 18–55 m for 4–19 days. The value of the total organic carbon flux (TOC) contained in the cells of bacteria (BAC), heterotrophic nanoflagellates (HNF) and virus particles (VIR) in the ESS varied from 0.5 to 2.4 mg C m–2 day–1 and amounted to 1.1–4.9% of the total TOC flux, in the LS – from 0.7 to 5.2 mg C m–2 day–1 and amounted to 1.1–6.2% of the total TOC flux. The maximum values of flows were measured near the Lena River delta, the mouths of the Khatanga and Indigirka Rivers. The contribution of BAC, GNF and VIR to the total biomass of the microbial community attached to sinking particles was, on average, 59 ± 11%, and 28 ± 8%, 13 ± 9% for VSM and ML, respectively.

Толық мәтін

Рұқсат жабық

Авторлар туралы

A. Kopylov

Papanin Institute for Biology of Inland Waters, Russian Academy of Sciences; Shirshov Institute of Oceanology, Russian Academy of Sciences

Хат алмасуға жауапты Автор.
Email: kopylov@ibiw.ru
Ресей, Borok; Moscow

Е. Zabotkina

Papanin Institute for Biology of Inland Waters, Russian Academy of Sciences

Email: kopylov@ibiw.ru
Ресей, Borok

А. Romanenko

Papanin Institute for Biology of Inland Waters, Russian Academy of Sciences

Email: kopylov@ibiw.ru
Ресей, Borok

А. Sazhin

Shirshov Institute of Oceanology, Russian Academy of Sciences

Email: kopylov@ibiw.ru
Ресей, Moscow

M. Flint

Shirshov Institute of Oceanology, Russian Academy of Sciences

Email: kopylov@ibiw.ru
Ресей, Moscow

Әдебиет тізімі

  1. Дриц А.В., Кравчишина М.Д., Пастернак А.Ф. и др. Роль зоопланктона в вертикальном потоке вещества в Карском море и море Лаптевых в осенний сезон // Океанология. 2017. Т. 57. № 6. с. 934–948. https://doi.org/10.7868/S0030157417060089
  2. Дриц А.В., Пастернак А.Ф., Кравчишина М.Д. и др. Роль планктона в вертикальном потоке вещества на шельфе Восточно-Сибирского моря // Океанология. 2019. Т. 59. № 5. С. 746–754. https://doi.org/10.31857/S0030-1574595746-754
  3. Дриц А.В., Кравчишина М.Д., Суханова И.Н. и др. Сезонная изменчивость потока осадочного вещества на шельфе северной части Карского моря // Океанология. 2021. Т. 61. № 6. С. 984–993. https://doi.org/10.1134/S0001437021060217
  4. Косолапова Н.Г., Косолапов Д.Б., Копылов А.И., Романенко А.В. Гетеротрофные нанофлагелляты в пелагиали и донных отложениях восточной части моря Лаптевых // Океанология. 2019. Т. 59. № 6. С. 974–986. https://doi.org/10.31857/S0030-1574596974-986
  5. Лисицын А.П., Новигатский А.Н., Клювиткин А.А. и др. Потоки рассеянного вещества в Белом море, седиментационные обсерватории, новые направления изучения осадочного вещества // Система Белого моря. Т. 3. М.: Научный мир, 2013. С. 201–291.
  6. Лукашин В.Н., Клювиткин А.А., Лисицын А.П., Новигатский А.Н. Малая седиментационная ловушка МСЛ-110 // Океанология. 2011. Т. 51. № 4. С. 746–750.
  7. Люцарев С.В., Сметанкин А.В. Определение углерода в водной взвеси // Методы исследования органического вещества в океане. М.: Наука, 1980. С. 46–50.
  8. Пастернак А.Ф., Дриц А.В., Кравчишина М.Д., Флинт М.В. Вклад зоопланктона в вертикальный поток вещества в морях Сибирской Арктики // Докл. РАН. 2017. Т. 477. № 3. С. 380–383. https://doi.org/10.7868/S086956521733026X
  9. Суханова И.Н., Флинт М.В. Сезонная динамика вертикальных потоков фитопланктона, тинтиннид и стрекательных клеток кишечнополостных в Карском море // Океанология. 2022. Т. 62. № 6. С. 887–897. https://doi.org/10.31857/S0030157422060120
  10. Alcolombri U., Peaudecent F.J., Fernandez V.I. et al. Sinking enhances the degradation of organic particles by marine bacteria // Nature Geoscience. 2021. V. 14. P. 775–780. https://doi.org/10.1038/s41561-021-00817-x
  11. Azam F., Malfatti F. Microbial structuring of marine ecosystems // Nat. Rev. Microbiol. 2007. V. 5. P. 782–791. https://doi.org/10.1038/nrmicro1747
  12. Baumas C., Bizic M. A focus on different types of organic matter particles and their significance in the open ocean carbon cycle // Progress in Oceanography. 2024. Vol. 224. P. e103233. https://doi.org/10.1016/j.pocean.2024.103233
  13. Binder B. Reconsidering the relationship between viral-ly induced bacterial mortality and frequency of infected cells // Aquat. Microb. Ecol. 1999. V. 18. P. 207–215. https://doi.org/10.3354/ame018207
  14. Børsheim K.Y., Bratbak G. Cell volume to carbon conversion factors for bacterivorous Monas sp. enriched from seawater // Mar. Ecol. Prog. Ser. 1987. V. 36. P. 171–175. https://doi.org/10.3354/meps036171
  15. Caron D.A. Technique for enumeration of heterotro-phic and phototrophic nanoplankton, using epifluores-cence microscopy and comparison with other procedures // Appl. Environ. Microbiol. 1983. V. 46. № 2. P. 491–498. https://doi.org/10.1128/aem.46.2.491-498.1983
  16. Drits A.V., Pasternak A.F., Arashkevich E.G. et al. Influence of riverine discharge and timing of ice retreat on particle sedimentation patterns on the Laptev Sea shelf // J. Geo. Res. Ocean. 2021. V. 126. Art. e2021JC017462. https://doi.org/10.1029/2021JC017462
  17. Ducklow H.W., Hill S.M., Gardner W.G. Bacterial growth and the decomposition of particulate organic carbon collected in sediment traps // Continent. Shelf Res. 1985. V. 4. N4. P. 445–464. https://doi.org//10.1016/0278-4343(85)90004-4
  18. Fontanez K.M., Eppley J.M., Samo T.J. et al. Microbial community structure and function on sinking particles in the North Pacific Subtropical Gyre // Front Microbial. 2015. V. 6. Art. e469. https://doi.org/10.3389/fmicb.2015.00469
  19. Iturriaga R. Bacterial activity related to sedimenting particulate matter // Mar. Biol. 1979. V. 55. P. 157–169. https://doi.org/10.1007/BF00396814
  20. Jiao N., Hernal G.J., Hansell D.A. et al. Microbial production of recalcitrant dissolved organic matter: Long-term carbon storage in the global ocean // Nat. Rev. Microbiol. 2010. V. 8. P. 593–599. https://doi.org/10.1038/nrmicro2386
  21. Jover L.F., Effler T.C., Buchan A. et al. The elemental composition of virus particles: implications for marine biogeochemical cycles // Nat. Rev. Microbiol. 2014. V. 12. P. 519–528. https://doi.org/10.1038/nrmicro3289
  22. Kiørboe, T., Grossart, H.P., Ploug, H. et al. Particle-associated flagellates: swimming patters, colonization rates and grazing on attached bacteria // Aquat. Microb. Ecol. 2004. V. 35. P. 141–152. https://doi.org/10.3354/ame035141
  23. Kopylov A.I., Zabotkina E.A., Romanenko A.V. et al. Viruses in the water column and the sediment of the eastern part of the Laptev Sea // Est. Coast. Shelf Sci. 2020. V. 242. Art. e106836. https://doi.org/10.1016/j.ecss.2020.106836
  24. Kopylov A.I., Zabotkina E.A., Kosolapov D.B. et al. Viruses and viral infection of heterotrophic prokaryotes in shelf waters of the western part of the East Siberian Sea // J. Mar. Sys. 2021. V. 218. Art. e103544. https://doi.org/10.1016/j.jmarsys.2021.103544
  25. Luo E., Leu A.O., Eppley J.M. et al. Diversity and origins of bacterial and archaeal viruses on sinking particles reaching the abyssal ocean // ISME J. 2022. V. 16. P. 1668–1675. https://doi.org/10.1038/s41396-022-01224-9
  26. Mestre M., Ruiz-Gonzalez C., Logares R. et al. Sinking particles promote vertical connectivity in the ocean microbiome // Proc. Nat. Acad. Sci. USA. 2018. V. 115. P. E6799–E6807. https://doi.org/10.1073/pnas.1802470115
  27. Norland S. The relationship between biomass and volume of bacteria. In Handbook of Methods in Aquatic Microbial Ecology, ed. Kemp, P. F., Cole, J. J., Sherr, B. F., Sherr, E.B. Lewis Publ.: Boca Raton, 1993. P. 303–308. https://doi.org/10.1201/9780203752746-36
  28. Passow U., Carlson C.A. The biological pump in a high CO2 world // Mar. Ecol. Prog. Ser. 2012. V. 470. P. 249–271. https://doi.org/10.3354/meps09985
  29. Porter K.G., Feig Y.S. The use DAPI for identifying and counting of aquatic microflora // Limnol. Oceanogr. 1980. V. 25. № 5. P. 943–948. https://doi.org/10.4319/lo.1980.25.5.0943
  30. Proctor L.M., Fuhrman J.A. Roles of viral infection in organic particles flux // Mar. Ecol. Prog. Ser. 1991. V. 69. P. 133–142.
  31. Shen Y., Guilderson T.P., Chavez F.P., McCarthy D. Important contribution of bacteria carbon and nitrogen to sinking particle export // Geophys. Res. Lett. 2023. V. 50. e11. https://doi.org/10.1029/2022GL102485
  32. Suttle C.A. Enumeration and isolation of virus. In: Kemp P.F., Cole J.J., Sherr B.F., Sherr E.B. (Eds). Handbook of Methods in Aquatic Microbial Ecology. 1st Ed: Lewis Publisher: Boca Raton, 1993. 121–13 pp.
  33. Taylor G.Y., Kari D.M., Pace M.L. Impact of bacteria and zooflagellates on the composition of sinking particles: an in situ experiment // Mar. Ecol. Progr. Ser. 1986. V. 29. № 2. P. 144–155.
  34. Turley C., Mackie P. Biogeochemical significance of attached and free-living bacteria and the flux of particles in the NE Atlantic Ocean // Mar. Ecol. Progr. Ser. 1994. V. 115. P. 191–203. https://doi.org/10.3354/meps115191
  35. Valencia B., Stukel M.R., Allen A.E. et al. Microbial communities with sinking particles across an environmental upwelling to the oligotrophic ocean // Deep Sea Res. P.I. Oceanogr. Res. Pap. 2022. V. 179. Art. e103668. https//doi.org/10/1016/j.dsr.2021.103668
  36. Wrobel В., Filippini M., Piwowarczyk J. et al. Low virus to prokaryote ratios in the cold: benthic viruses and prokaryotes in a subpolar marine ecosystem (Hornsund, Svalbard) // Int. Microbiol. 2013. V. 16. P. 45–52. https:// doi.org/10.2436/20.1501.01.179

Қосымша файлдар

Қосымша файлдар
Әрекет
1. JATS XML
Жүктеу
2. Fig. 1. The station layout map.

Жүктеу (278KB)
Метадеректер
3. 2. Electron micrographs of dividing bacteria (a, b), free viruses (c, d), viruses attached to bacterial cells (e), viruses attached to the surface of detritus particles (e, g), viruses inside infected bacterial cells (h, i), in microbial communities, associated with sinking particles.

Жүктеу (480KB)
Метадеректер

© Russian Academy of Sciences, 2025