Study of the Activation Process of Isotope Exchange between Boc-Trp-Pro-Pro-Trp, Trp-Pro-Pro-Trp and Deuterated Water
- 作者: Shevchenko V.P.1, Shevchenko K.V.1, Nagaev I.Y.1, Andreeva L.A.1
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隶属关系:
- National Research Centre Kurchatov Institute
- 期: 卷 66, 编号 3 (2024)
- 页面: 298-303
- 栏目: Articles
- URL: https://journals.eco-vector.com/0033-8311/article/view/681643
- DOI: https://doi.org/10.31857/S0033831124030123
- ID: 681643
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详细
The conditions for introducing deuterium into Trp-Pro-Pro-Trp and Boc-Trp-Pro-Pro-Trp by isotope exchange with D2O in the presence of a 5% Pd/Al2O3 catalyst were developed. The thermal stability of Trp-Pro-Pro-Trp upon introduction of deuterium was significantly lower than that of the Boc-protected peptide. The yield of deuterated Trp-Pro-Pro-Trp did not exceed 10–15%, and the inclusion of deuterium was about 0.9–1.1 at./molecule at 125°C. The introduction of deuterium into Boc-Trp-Pro-Pro-Trp was possible at 150°C, which increased the deuterium content to 3.75 at./molecule. It was found that the tryptophan fragment of the molecule contains approximately 2 times more deuterium than the proline fragment. It was found that pretreatment of Boc-Trp-Pro-Pro-Trp with deuterium gas not only activates isotope exchange, but also promotes the removal of Boc protection. The data obtained indicate that the reason for this phenomenon is associated with the generation of deuterium cations on Boc-Trp-Pro-Pro-Trp molecules by the catalyst.
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作者简介
V. Shevchenko
National Research Centre Kurchatov Institute
编辑信件的主要联系方式.
Email: nagaev.img@yandex.ru
俄罗斯联邦, pl. Kurchatova 2, Moscow, 123182
K. Shevchenko
National Research Centre Kurchatov Institute
Email: nagaev.img@yandex.ru
俄罗斯联邦, pl. Kurchatova 2, Moscow, 123182
I. Nagaev
National Research Centre Kurchatov Institute
Email: nagaev.img@yandex.ru
俄罗斯联邦, pl. Kurchatova 2, Moscow, 123182
L. Andreeva
National Research Centre Kurchatov Institute
Email: nagaev.img@yandex.ru
俄罗斯联邦, pl. Kurchatova 2, Moscow, 123182
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