Omsk State Medical University, Ministry of Health of Russia

Cover Page

Cite item

Full Text

Open Access Open Access
Restricted Access Access granted
Restricted Access Subscription or Fee Access

Abstract

Purpose. To evaluate the ability of neutrophils to form extracellular traps in children with latent TB infection with different sensitivity to recombinant tuberculosis allergen (ATR) to form an individual approach to therapeutic and preventive measures.

Material and methods. The study involved 22 children with a moderate and mild reaction to ATP (group 1) and 31 children with a pronounced reaction (group 2).

Results. According to the general clinical blood test, there were no differences between the groups. The median of intact neutrophils in group 1 was 52.0, in group 2 – 35.6 (p=0.251). The median of hyperactivated neutrophils in group 1 was 10.7, in group 2 – 2.9 (p=0.0295).

Conclusion. In the group with a pronounced reaction to ATR (group 2), there was an increased readiness of neutrophils to form extracellular traps (the number of early NETosis cells exceeds that in the comparison group) and a greater number of formed cloud-like neutrophil extracellular traps (incomplete NETosis). At the same time, in children with a calmer reaction to the test with ATR (weak and moderately expressed), the readiness of neutrophils to form extracellular traps is observed, but at the same time hyperactivation of the neutrophil and the formation of full-fledged thread-like neutrophil traps are achieved.

Full Text

Restricted Access

About the authors

A. Mordyk

Omsk State Medical University, Ministry of Health of Russia

Author for correspondence.
Email: rmari1@mail.ru
ORCID iD: 0000-0001-6196-7256

MD, Professor

Russian Federation, Omsk

M. A. Romanova

Omsk State Medical University, Ministry of Health of Russia

Email: rmari1@mail.ru
ORCID iD: 0000-0002-1775-607X

Candidate of Medical Sciences

Russian Federation, Omsk

A. N. Zolotov

Omsk State Medical University, Ministry of Health of Russia

Email: rmari1@mail.ru
ORCID iD: 0000-0002-6775-323X

Candidate of Medical Sciences; Associate Professor

Russian Federation, Omsk

D. G. Novikov

Omsk State Medical University, Ministry of Health of Russia

Email: rmari1@mail.ru
ORCID iD: 0000-0002-4339-2222

Candidate of Medical Sciences

Russian Federation, Omsk

N. A. Kirichenko

Omsk State Medical University, Ministry of Health of Russia

Email: rmari1@mail.ru
ORCID iD: 0000-0002-8411-0973
Russian Federation, Omsk

A. O. Ptukhin

Omsk State Medical University, Ministry of Health of Russia

Email: rmari1@mail.ru
Russian Federation, Omsk

References

  1. Васильева И.А., Тестов В.В., Стерликов С.А. Эпидемическая ситуация по туберкулезу в годы пандемии COVID-19 – 2020-2021 гг. Туберкулез и болезни легких. 2022; 100 (3): 6–12 [Vasilyeva I.А., Testov V.V., Sterlikov S.А. Tuberculosis situation in the years of the COVID-19 pandemic – 2020–2021. Tuberculosis and Lung Diseases. 2022; 100 (3): 6–12 (in Russ.)]. doi: 10.21292/2075-1230-2022-100-3-6-12
  2. Аксенова В.А., Барышникова Л.А., Клевно Н.И. и др. Новые возможности скрининга и диагностики различных проявлений туберкулезной инфекции у детей и подростков в России. Вопросы современной педиатрии. 2011; 10 (4): 16–22 [Aksenova V., Barishnikova L., Klevno N. et al. New screening and diagnostic options of various traits of TB infection among children and adolescents in Russia. Current Pediatrics. 2011; 10 (4): 16–22 (in Russ.)].
  3. Аксенова В.А., Барышникова Л.А., Долженко Е.Н. и др. Актуальные вопросы массового обследования детского населения на туберкулез в современных условиях. Доктор.Ру. 2012; 76 (8): 27–9 [Aksionova V.A., Baryshnikova L.A., Dolzhenko E.N. et al. Tuberculosis Screening in Children Today: Important Issues. Doctor.Ru. 2012; 76 (8): 27–9 (in Russ.)].
  4. Литвинов В.И. «Дремлющие» микобактерии, дормантные локусы, латентная туберкулезная инфекция. Туберкулез и социально-значимые заболевания. 2016; 2: 5–13 [Litvinov V.I. «Dormant» mycobacteria, dormant loci, latent tuberculosis infection. Tuberculosis and socially significant diseases. 2016; 2: 5–13 (in Russ.)].
  5. Поддубная Л.В., Шилова Е.П., Кудлай Д.А. и др. Иммунодиагностические тесты в оценке специфической сенсибилизации организма М. tuberculosis у детей в современных эпидемических условиях. Туберкулез и болезни легких. 2021; 99 (11): 47–55 [Poddubnаya L.V., Shilovа E.P., Kudlаy D.A. et al. Immunodiagnostic Tests in the Assessment of Specific Sensitization to M. tuberculosis in Children under the Current Epidemical Situation. Tuberculosis and Lung Diseases. 2021; 99 (11): 47–54 (in Russ.)]. doi: 10.21292/2075-1230-2021-99-11-47-54
  6. Старшинова А.А., Кудлай Д.А., Довгалюк И.Ф. и др. Эффективность применения новых методов иммунодиагностики туберкулезной инфекции в Российской Федерации. Педиатрия им. Г.Н. Сперанского. 2019; 98 (4): 229–35 [Starshinova А.А., Dovgalyuk I.F., Yablonsky P.K. et al. Efficacy of new methods of tuberculosis infection immunodiagnostics in the Russian Federation. Pediatria n.a. G.N. Speransky. 2019; 98 (4): 229–35 (in Russ.)]. doi: 10.24110/0031-403X-2019-98-4-229-235
  7. Екатеринчева О.Л., Малкова А.М., Карев В.Е. и др. Особенности диагностики туберкулеза на фоне COVID-19. Журнал инфектологии. 2021; 13 (1): 117–23 [Ekaterincheva O.L., Malkova A.M., Karev V.E. et al. Features of tuberculosis diagnosis in the COVID-19. Journal Infectology. 2021; 13 (1): 117–23 (in Russ.)]. doi: 10.22625/2072-6732-2021-13-1-117-123
  8. Павленок И.В., Зорина М.М., Пушкарева Е.Ю. и др. Организация профилактических противотуберкулезных мероприятий: учебное пособие. Новосибирск: ФГБУ «ННИИТ» Минздрава России, 2022; с. 160–4 [Pavlenok I.V., Zorina M.M., Pushkareva E.Ju. et al. Organization of preventive anti-tuberculosis measures: training manual. Novosibirsk. FGBU «NNIIT» Ministry of Health of Russia, 2022; pp. 160–4 (in Russ.)].
  9. Фелькер И.Г., Павленок И.В., Ставицкая Н.В. и др. Латентная туберкулезная инфекция среди детей и взрослых в регионах с высокой распространенностью туберкулеза. Туберкулез и болезни легких. 2023; 101 (1): 34–40 [Felker I.G., Pavlenok I.V., Stavitskaya N.V. et al. Latent Tuberculosis Infection Among Children and Adults in the Regions with High Prevalence of Tuberculosis. Tuberculosis and Lung Diseases. 2023; 101 (1): 34–40 (in Russ.)]. doi: 10.58838/2075-1230-2023-101-1-34-40
  10. Slogotskaya L.V., Litvinov V., Ovsyankina E. et al. Results of QuantiFERON-TB Gold in-tube and skin testing with recombinant proteins CFP-10-ESAT-6 in children and adolescents with TB or latent TB infection. Paediatric Respiratory Reviews. 2013; 14 (2): S65.
  11. Slogotskaya L.V., Bogorodskaya E., Sentchichina O. et al. Effectiveness of tuberculosis detection using a skin test with allergen recombinant (CFP-10-ESAT-6) in children. European Respiratory Journal. 2015; 46 (Suppl. 59): РА4524.
  12. Shovkun L., Aksenova V., Kudlay D. et al. The role of immunological tests in the diagnosis of tuberculosis infection in children with juvenile idiopathic arthritis (JIA). European Respiratory Journal. 2018; 52 (Suppl. 62): PA2733.
  13. Slogotskaya L.V., Bogorodskaya Е., Ivanova D. et al. Sensitivity and specificity of new skin test with recombinant protein CFP10-ESAT6 in patients with tuberculosis and individuals with non- tuberculosis diseases. European Respiratory Journal. 2013; 42 (Suppl. 57): 1995.
  14. Аксенова В.А., Васильева И.А., Клевно Н.И. и др. Валидность отрицательного результата кожного теста с аллергеном туберкулезным рекомбинантным для исключения активного туберкулеза у детей. Педиатрия. Журнал им. Г.Н. Сперанского. 2022; 101 (4): 43–9 [Aksenova V.A., Vasilyeva I.A., Klevno N.I. et al. Validity of a negative skin test result with recombinant tuberculosis allergen in order to exclude the active tuberculosis in children. Pediatria n.a. G.N. Speransky. 2022; 101 (4): 43–9 (in Russ.)]. doi: 10.24110/0031-403X-2022-101-4-43-49
  15. Vasilyeva I.A., Aksenova V.A., Kazakov A.V. et al. Evaluation of the specificity of an intradermal test with recombinant tuberculosis allergen in bacillus Calmette-Guérin-vaccinated healthy volunteers. Front Med (Lausanne). 2023; 10: 1042461. doi: 10.3389/fmed.2023.1042461
  16. Новиков Д.Г., Золотов А.Н., Кириченко Н.А. и др. Способ обнаружения нейтрофильных внеклеточных ловушек в суправитально окрашенном препарате крови. Патент на изобретение № 2768152. Опубл. 23.03.2022, Бюл. №9 [Novikov D.G., Zolotov A.N., Kirichenko N.A. et al. Method for detection of neutrophil extracellular traps in supravital stained blood preparation. Patent for invention № 2768152. Publ. 23.03.2022, Bull. №9 (in Russ.)].

Supplementary files

Supplementary Files
Action
1. JATS XML
Download

Copyright (c) 2025 Russkiy Vrach Publishing House