Mast cells as predictors of bladder cancer

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Abstract

Objective. To investigate mast cell populations in patients of different ages with Stage li-ill bladder cancer (BC), by using innovative immunohistochemical approaches (to study chymase and thymase of positive structures). Subjects and methods. Immunohistochemical approaches were used to examine material from 25 patients: a study group of 20 patients with histologically verified Stage ll-lll bladder cancer; a control group of 5 men aged 36-50years without cancer who died in traffic accidents. Results. The investigation ofthe protease profile of a mast cell population in the bladder showed that during the process of cancer development, the time course of changes In the content of protease-posltive mast cells was different: the number of tryptase-positlve mast cells Increased, while that of chymase-positive mast cells significantly decreased. An analysis ofthe protease profile indicated that with the process of cancer development, mast cell chymase expression noticeably decreased; the size of mast cells in the connective tissue subpopulation reduced. However, they could form local intraorganlc accumulations, secreting both tryptase and chymase in fairly immense areas.

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About the authors

T. V. Pavlova

Belgorod State National Research University

Author for correspondence.
Email: pavlova@bsu.edu.ru

доктор медицинских наук, профессор

D. A. Atyakshin

N.N. Burdenko Voronezh State Medical University

Email: pavlova@bsu.edu.ru

Research Institute of Experimental Biology and Medicine

I. A. Pavlov

Belgorod State National Research University

Email: pavlova@bsu.edu.ru

кандидат медицинских наук

D. V. Bessmertny

Belgorod State National Research University

Email: pavlova@bsu.edu.ru

кандидат медицинских наук

N. B. Pilkevich

Belgorod State National Research University

Email: pavlova@bsu.edu.ru

доктор медицинских наук, профессор

K. I. Proshchaev

Belgorod State National Research University

Email: pavlova@bsu.edu.ru

доктор медицинских наук, профессор

References

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2. Fig. 1. Transitional cell BC (a male patient aged 55 years); the tissue consists of cells with impaired polarity, which have lost intercellular contacts with large hyperchromatic nuclei; there are commonly mitotic figures that are often atypical; H&E; Fragment One: a – ×250; б – ×100; Fragment Two: в – ×100; г – ×400

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3. Fig. 2. Bladder fragments in the control group: a, б – a male patient aged 40 years; the location of mast cells in the endomysium; the pericapillary location in the bladder muscle layer; active granular secretion (б – the protease profile of a mast cell population [tryptase]); в, г – a male patient aged 45 years

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4. Fig. 3. A bladder fragment in BC: a, б – a male patient aged 50 years; the mast cells are located in the area of the bladder stroma, as well as the basement membrane of the atypical bladder epithelium; active tryptase secretion; there are a large number of secretory granules; secretion of individual tryptase-positive granules into the extracellular matrix; selective secretion of mast cell tryptase to the stromal cell nucleus (б – the protease profile of a mast cell population [tryptase]); в, г – a female patient aged 65 years; chymase-positive mast cells are located in the peritumoral area of the bladder stroma; the high content of chymase-positive granules in the mast cell cytoplasm (г – the protease profile of a mast cell population [chymase])

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5. Fig. 4. Mast cells in bladder tissue (tryptase (green), chymase (red), DAPI staining (blue): a – a control group: a male patient aged 45 years; a mast cell with simultaneous content of tryptase and chymase; б – a study group: a male patient aged 50 years with BC; there is a mast cell with small granules, with simultaneous expression of chymase and tryptase; multiple immunolabeling of mast cell tryptase and chymase

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