Mast cells as predictors of bladder cancer

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Objective. To investigate mast cell populations in patients of different ages with Stage li-ill bladder cancer (BC), by using innovative immunohistochemical approaches (to study chymase and thymase of positive structures). Subjects and methods. Immunohistochemical approaches were used to examine material from 25 patients: a study group of 20 patients with histologically verified Stage ll-lll bladder cancer; a control group of 5 men aged 36-50years without cancer who died in traffic accidents. Results. The investigation ofthe protease profile of a mast cell population in the bladder showed that during the process of cancer development, the time course of changes In the content of protease-posltive mast cells was different: the number of tryptase-positlve mast cells Increased, while that of chymase-positive mast cells significantly decreased. An analysis ofthe protease profile indicated that with the process of cancer development, mast cell chymase expression noticeably decreased; the size of mast cells in the connective tissue subpopulation reduced. However, they could form local intraorganlc accumulations, secreting both tryptase and chymase in fairly immense areas.

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作者简介

T. Pavlova

Belgorod State National Research University

编辑信件的主要联系方式.
Email: pavlova@bsu.edu.ru

доктор медицинских наук, профессор

D. Atyakshin

N.N. Burdenko Voronezh State Medical University

Email: pavlova@bsu.edu.ru

Research Institute of Experimental Biology and Medicine

I. Pavlov

Belgorod State National Research University

Email: pavlova@bsu.edu.ru

кандидат медицинских наук

D. Bessmertny

Belgorod State National Research University

Email: pavlova@bsu.edu.ru

кандидат медицинских наук

N. Pilkevich

Belgorod State National Research University

Email: pavlova@bsu.edu.ru

доктор медицинских наук, профессор

K. Proshchaev

Belgorod State National Research University

Email: pavlova@bsu.edu.ru

доктор медицинских наук, профессор

参考

  1. Пристром M.C., Пристрои С.Л., Семенков И.И. Старение физиологическое и преждевременное: современный взгляд на проблему. Медицинские новости. 2015; 2:36-45.
  2. Онкогеронтология. Руководство для врачей. Под ред. В.Н. Анисимова, А.М. Беляева. СПб: Издательство AHHM0 «Вопросы онкологии», 2017; 509.
  3. Злокачественные новообразования в России в 2020 года (заболеваемость и смертность). Под ред. А.Д. Каприна, В.В. Старинского, А.О. Шахзадовой. М.: МНИОИ им. П.А. Герцена - филиал ФГБУ «НМИЦ радиологии» Минздрава России, 2021; 252 с.
  4. Павлова Т.В., Пилькевич Н.Б., Бессмертный Д.В. и др. Особенности метаболического атилизма при развитии онкологической патологии мочеполовой системы. Молекулярная медицина. 2021; 19 (31): 30-4. D01:10.29296/24999490-2021 -01-04
  5. Клинические рекомендации. Рак мочевого пузыря. Кодирование по Международной статистической классификации болезней и проблем, связанных со здоровьем: С67. Возрастная группа: взрослые. 2020; с. 81.
  6. Бухвалов И.Б., Атякшин Д.А, Павлова Т. В. и др. Гистохимия. Воронеж: Научная книга, 2018; 238 с.
  7. Павлова Т.В., Куликовский В.Ф., Павлова Л.А. Клиническая и экспериментальная морфология. М.: ООО Медицинское информационное агентство, 2018; 256 с.
  8. Buchwalow, I.B., Boecker W. Immunohistochemistry: Basics and Methods, 19 ed. Berlin, Heidelberg (Germany), Dordrecht (Netherlands), London (UK), New York (USA): Springer, 2010; 152 p.
  9. Atiakshin D.A., Shishkina V.V., Gerasimova O.A. et al.Combined histochemical approach in assessing tryptase expression in the mast cell population. Acta Histochem. 2021; 123:151-71. doi: 10.1016/j.acthis.2021.151711

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2. Fig. 1. Transitional cell BC (a male patient aged 55 years); the tissue consists of cells with impaired polarity, which have lost intercellular contacts with large hyperchromatic nuclei; there are commonly mitotic figures that are often atypical; H&E; Fragment One: a – ×250; б – ×100; Fragment Two: в – ×100; г – ×400

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3. Fig. 2. Bladder fragments in the control group: a, б – a male patient aged 40 years; the location of mast cells in the endomysium; the pericapillary location in the bladder muscle layer; active granular secretion (б – the protease profile of a mast cell population [tryptase]); в, г – a male patient aged 45 years

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4. Fig. 3. A bladder fragment in BC: a, б – a male patient aged 50 years; the mast cells are located in the area of the bladder stroma, as well as the basement membrane of the atypical bladder epithelium; active tryptase secretion; there are a large number of secretory granules; secretion of individual tryptase-positive granules into the extracellular matrix; selective secretion of mast cell tryptase to the stromal cell nucleus (б – the protease profile of a mast cell population [tryptase]); в, г – a female patient aged 65 years; chymase-positive mast cells are located in the peritumoral area of the bladder stroma; the high content of chymase-positive granules in the mast cell cytoplasm (г – the protease profile of a mast cell population [chymase])

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5. Fig. 4. Mast cells in bladder tissue (tryptase (green), chymase (red), DAPI staining (blue): a – a control group: a male patient aged 45 years; a mast cell with simultaneous content of tryptase and chymase; б – a study group: a male patient aged 50 years with BC; there is a mast cell with small granules, with simultaneous expression of chymase and tryptase; multiple immunolabeling of mast cell tryptase and chymase

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