Role of blood iron-binding proteins in antioxidant protection and their association with genital endometriosis


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Abstract

The structural and functional characteristics of iron-binding proteins and their contribution to the formation of oxidant stress in genital endometriosis were examined, by studying plasma samples from 22 patients, peritoneal fluid from 12 patients and plasma from 8 apparently healthy women. Electron paramagnetic resonance spectroscopy was used to determine the active centers of the major iron-binding proteins ceruloplasmin (CP) and transferrin (TF) and their ratio as a criterion for evaluating the plasma antioxidant status and to study the blood TF and lactoferrin in the perineal fluid. The ratio of the active CL/TF centers was found to be statistically significantly higher in endometriosis. Wide signal spectra of iron complexes were recorded in 76% and changes in the signal waveform of lactoferrin were observed in all cases. The revealed changes support the impaired mechanisms of antioxidative defense with the development of oxidative stress at the systemic and local levels. The findings confirm the important implication of oxidant stress in the pathogenesis of genital endometriosis and substantiate new therapeutic and pharmacological strategies, by using antioxidants and anti-inflammatory agents in the combined treatment of the disease.

About the authors

L V Adamyan

Academician V. I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Russian Agency for Medical Technologies, Moscow

Email: ncagp@bk.ru
Academician V. I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Russian Agency for Medical Technologies, Moscow

E N Burgova

Moscow State Medical University, Russian Agency for Health Care

Moscow State Medical University, Russian Agency for Health Care

V A Serezhenkov

Moscow State Medical University, Russian Agency for Health Care

Moscow State Medical University, Russian Agency for Health Care

M M Sonova

N. N. Semenov Institute of Chemical Physics, Russian Academy of Sciences

N. N. Semenov Institute of Chemical Physics, Russian Academy of Sciences

A A Osipova

N. N. Semenov Institute of Chemical Physics, Russian Academy of Sciences

N. N. Semenov Institute of Chemical Physics, Russian Academy of Sciences

K N Arslanyan

N. N. Semenov Institute of Chemical Physics, Russian Academy of Sciences

N. N. Semenov Institute of Chemical Physics, Russian Academy of Sciences

O V Poddubnaya

N. N. Semenov Institute of Chemical Physics, Russian Academy of Sciences

N. N. Semenov Institute of Chemical Physics, Russian Academy of Sciences

References

  1. Адамян Л. В., Спицын В. А., Андреева Е. Н. Генетические аспекты гинекологических заболеваний. - М., 1999.
  2. Адамян Л. В., Гаспарян С. А. Генитальный эндометриоз. Современный взгляд на проблему. - Ставрополь, 2004.
  3. Адамян Л. В., Кулаков В. И., Андреева Е. Н. Эндометриозы: Руководство для врачей. - М., 2006.
  4. Баскаков В. П., Цвелев Ю. В., Кира Е. Ф. Эндометриоидная болезнь. - СПб., 2002.
  5. Коган А. Х., Грачев С. В., Елисеева С. В. Модулирующая роль СО2 в действии активных форм кислорода. - М., 2006.
  6. Окислительный стресс. Прооксиданты и антиоксиданты / Меньшикова Е. Б., Ланкин В. З., Зенков Н. К. и др. - М., 2006.
  7. Посисеева Л. В., Назарова А. О., Назаров С. Б. // Акуш. и гин. - 1999. - № 6. - С. 37-40.
  8. Brock J. H. // Biochem. Cell Biol. - 2002. - Vol. 80. - P. 1-6.
  9. Cao X., Yang D., Song M. et al. // Fertil. and Steril. - 2004. - Vol. 82. - P. 999-1007.
  10. Elass-Rochard E., Roseanu A., Legrand D. et al. // Biochem. J. - 1995. - Vol. 312. - P. 839-845.
  11. Foster W. G., Agarwal S. K. // Ann. N. Y. Acad. Sci. - 2002. - Vol. 955. - P. 213-232.
  12. Gupta S., Agarwal A., Krajcir N., Alvarez J. G. // Reprod. BioMed. Online. - 2006. - Vol. 13, N 1. - P. 126-134.
  13. Haider R. C. // Eur. J. Clin. Invest. - 2002. - Vol. 32, N 1. - P. 50-54.
  14. Hayashida K., Takeuchi T., Ozaki T. et al. // Am. J. Physiol. Regul. Integr. Comp. Physiol. - 2004. - Vol. 286. - P. 359-365.
  15. Koninckx P. R., Kennedy S. H., Barlow D. H. // Gynecol. Obstetr. Invest. - 1999. - Vol. 47, N 1. - P. 23-33.
  16. Kuipers M. E., Heegsma J., Bakker H. L. et al. // Drug Deliv. - 2002. - Vol. 9. - P. 31-38.
  17. Kumar S., Bandyopadhyay U. // Toxicol. Lett. - 2005. - Vol. 157. - P. 175-188.
  18. Legrand D., Elass E., Carpentier M., Mazurier J. // Cell. Mol. Life Sci. - 2005. - Vol. 62. - P. 2549-2559.
  19. Murphy A. A. // Ann. N. Y. Acad. Sci. - 2002. - Vol. 955. - P. 1-10.
  20. Omata Y., Satake M., Maeda R. // J. Vet. Med. Sci. - 2001. - Vol. 63. - P. 187-190.
  21. Rier S., Foster W. G. // Semin. Reprod. Med. - 2003. - Vol. 21, N 2. - P. 145-154.
  22. Teng C. T., Beard C., Gladwell W. // Biol. Reprod. - 2002. - Vol. 67. - P. 1439-1449l.
  23. Van Langendonckt A., Casanas-Roux F., Donnez J. // Fertil. and Steril. - 2002. - Vol. 77. - P. 861-870.

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