PROAND ANTIANGIOGENIC FACTORS IN THE PATHOGENESIS OF EARLY PREGNANCY LOSSES. PART I. THE SPECIFIC FEATURES OF PROAND ANTIANGIOGENIC SERUM FACTORS IN EARLY PREGNANCY


Cite item

Full Text

Open Access Open Access
Restricted Access Access granted
Restricted Access Subscription or Fee Access

Abstract

Objective. To determine the serum levels of angiogenic and antiangiogenic factors as the indicators reflecting the mechanisms of pathological reactions in early pregnancy losses. Subjects and methods. Sixty-six patients with early pregnancy were examined. The soluble forms of VEGF, VEGF-RI (sFlt-1), VEGF-R2 (sKDR), MMP-2, MMP-9, TIMP-1, TIMP-2, PLGF, and endoglin (sCDI05) were determined in their sera by enzyme immunoassay, by using the standard test systems (Bender MedSystems GmbH (Austria) and R&D Systems (USA). Results. MMP and their tissue inhibitors have a dominant role in pregnancy losses before 6 and 7—8 weeks of gestation. There was a significant reduction in PLGF and sVEGF-RI levels in pregnancy losses at II—I4 weeks gestation. Conclusion. The findings suggest that there are different immunological mechanisms responsible for abnormality in pregnancy in its early periods.

Full Text

Restricted Access

About the authors

M. M ZIGANSHINA

Academician V.I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health and Social Development of Russia

Email: mmz@mail.ru
Moscow

L. V KRECHETOVA

Academician V.I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health and Social Development of Russia

Moscow

L. V VANKO

Academician V.I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health and Social Development of Russia

Moscow

Z. S KHODZHAYEVA

Academician V.I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health and Social Development of Russia

Moscow

E. V MUSIYENKO

Academician V.I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health and Social Development of Russia

Moscow

G. T SUKHIKH

Academician V.I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health and Social Development of Russia

Moscow

References

  1. Бурлев В.А., Зайдиева З.С., Ильясова Н.А. Регуляция ангиогенеза гестационного периода // Пробл. репрод. - 2008. - № 3. - С. 15-22.
  2. Внутриутробное развитие человека / Под ред. А.П. Милованова, С.В. Савельева. - М.: Медицина для всех, - 2006.
  3. Лазюк Г.И. Тератология человека. - М.: Медицина, 1991.
  4. Ранние сроки беременности / Под ред. В.Е. Радзинского, А.А. Оразмурадова. - М.: Status Praesens, 2009.
  5. Bai S.X., Wang Y.L., Qin L. et al. Dynamic expression of matrix metalloproteinases (MMP-2,-9 and -14) and the tissue inhibitors of MMPs (TIMP-1,-2 and -3) at the implantation site during tubal pregnancy // Reproduction. - 2005. -№ 129. - P. 103-113.
  6. Bates D.O. Vascular endothelial growth factors and vascular permeability // Cardiovasc. Res. - 2010. - № 87. -P. 262-271.
  7. Burton G.I., Charnock-Jones D.S., Jauniaux E. Regulation of vascular growth and function in the human placenta // Reproduction. - 2009. - № 138. - P. 895-902.
  8. Douglas N.C., Tang H., Gomez R. et al. Vascular endothelial growth factor receptor 2 (VEGF-2) functions to promote uterine decidual angiogenesis during early pregnancy in the mouse // Endocrinology. - 2009. - № 150. - P. 3845-3854.
  9. Florio P., Gabbanini M., Borges L.E. et al. Activins and related proteins in the establishment of pregnancy // Reprod. Sci. -2010. - № 17. - P. 320-330.
  10. Forbes K., Westwood M. Maternal growth factor regulation of human placental development and fetal growth // J. Endocrinol. - 2010. - № 207. - P. 1-16.
  11. Gabhann F.M., Popel A.S. Model of competitive binding of vascular endothelial growth factor and placental growth factor to VEGF receptors on endothelial cells // Am. J. Physiol. Heart Circ. Physiol. 2004. - № 286. - P. 153-164.
  12. Kalkunte S.S., Mselle N.F., Norris W.E. et al. Vascular endothelial growth factor C facilitates immune tolerance and endovascular activity of human uterine NK cells at the maternal-fetal interface // J. Immunol. - 2009. - № 182. -P. 4085-4092.
  13. Lorquet S., Pequeux C., Berndt S. et al. Implication of VEGF receptor soluble forms, sVEGFR-1 and sVEGFR-2, in pathological angiogenesis // Abstracts of the 23rd Annual Meeting of the ESHRE, Lyon, France, 1-4 July, 2007. -P. 1174-1175.
  14. Molshkness T.A., Stouffer R.L., Burry K.A. Circulating levels of free and total vascular endothelial growth factor (VEGF)-A, soluble VEGF recepror-1 and -2, and angiogenin during ovarian stimulation in non-human primates and women // Hum. Reprod. - 2004. - № 19. - P. 822-830.
  15. Naruse K., Lash G.E., Innes B. et al. Localization of matrix metalloproteinase (MMP)-2, MMP-9 and tissue inhibitors for MMPs (TIMPs) in uterine natural killer cells in early human pregnancy // Hum. Reprod. - 2008. - Vol. 23, № 1. -P. 1-9.
  16. Olson M.W., Gervasi D.C., Mobashery S., Fridman R. Kinetic analysis of the binding of human matrix metalloproteinase-2 and -9 to tissue inhibitor of metalloproteinase (TIMP-1) and (TIMP-2) // J. Biol. Chem. - 1997. - № 272. -P. 29975-29983.
  17. Plaisier M., Streefland E., Koolwijk P. et al. Angiogenic growth factors and their receptors in first-trimester human deciduas of pregnancies further complicated by preeclampsia or fetal growth restriction // Reprod. Sci. - 2008. - № 15. -P. 720-726.
  18. Plaisier M., Dennert I., Rost E. et al. Decidual vascularization and the expression of angiogenic growth factors and proteases in first trimester spontaneous abortions // Hum. Reprod. - 2009. - Vol. 24. - P.185-197.
  19. Sugimoto H., Hamano Y., Charytan D. et al. Neutralization of circulating vascular endothelial growth factor (VEGF) by anti-VEGF antibodies and soluble VEGF receptor 1 (sFlt-1) induced proteinuria // J. Biol. Chem. - 2003. - № 278. -P. 12605-12608.
  20. Wang A., Rana S., Karumanchi S.A. Preeclampsia: the role of angiogenic factors in its pathogenesis // Physiology. - 2009. -№ 24. - P. 147-158.
  21. Wathen K-A., Tuutti E., Stenman U-H. et al. Maternal serum-soluble vascular endothelial growth factor receptor-1 in early pregnancy ending in preeclampsia or intrauterine growth retardation // J. Clin. Endocrinol. Metab. - 2006. -№ 91. - P. 180-184.
  22. Wulff C., Weigand M., Kreienberg R., Fraser H.M. Angiogenesis during primate placentation in health and disease // Reproduction. - 2003. - № 126. - P. 569-577.

Supplementary files

Supplementary Files
Action
1. JATS XML
Download

Copyright (c) 2012 Bionika Media