EVALUATION OF LOCAL VAGINAL INFLAMMATORY RESPONSE FROM THE PROFILE OF CYTOKINE GENE MRNA EXPRESSION IN PREGNANT WOMEN BEFORE BIRTH


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Abstract

Objective. То study whether the intensity of a local vaginal inflammatory process in women with full-term pregnancy before labor can be objectively estimated from the profile of cytokine gene mRNA expression. Subjects and methods. One hundred and one 19-to-40-year-old women with full-term pregnancy were examined 3—10 days before labor. Group 1 included 77 apparently healthy women with normocenosis without signs of vaginal inflammation. Forty-two of them had no complaints and 35 had profuse vaginal discharge. Group 2 comprised 24 pregnant women with clinical and laboratory signs of vaginitis. Cytokine gene mRNA expression was determined by real time RT-PCR. Results. The women with vaginitis were found to have significantly higher mRNA expression of the IL-1B, TNF, CD45, IL-6, LIF, IL-8, IL-10, and IFNG genes and lower levels of IL-12A, IL-18, TGF-B1 IL-12A, IL-18, and TGF-B1 than the healthy women. A mathematical model was developed for the objective diagnosis of a local inflammatory process from the expression of the IL-1B, IL-8, IL-10 and IL-18 genes. Conclusion. The cytokine gene expression profile may be used to detect an inflammatory response during vaginal dysbiotic processes in pregnant women before labor in order to prevent postpartum complications.

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About the authors

M. K MEDZHIDOVA

Academician V.I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology Ministry of Health and Social Development of Russia

Moscow

O. V BURMENSKAYA

Academician V.I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology Ministry of Health and Social Development of Russia

Moscow

A. E DONNIKOV

Academician V.I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology Ministry of Health and Social Development of Russia

Moscow

O. S NEPSHA

Academician V.I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology Ministry of Health and Social Development of Russia

Moscow

D. YU TROFIMOV

Academician V.I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology Ministry of Health and Social Development of Russia

Moscow

N. M KASABULATOV

Academician V.I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology Ministry of Health and Social Development of Russia

Moscow

V. L TYUTYUNNIK

Academician V.I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology Ministry of Health and Social Development of Russia

Moscow

G. T SUKHIKH

Academician V.I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology Ministry of Health and Social Development of Russia

Moscow

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