ROLE OF SYSTEMIC DISORDERS IN THE DEVELOPMENT OF GESTATIONAL COMPLICATIONS AND THEIR GENETIC CONSTITUENT


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Abstract

Objective. To study the role of the polymorphism of genes involved in the systemic processes of blood pressure regulation (AGT gene T803С, ACE gene i/D), in endothelial functions and oxidative stress (OS) (еNOS gene 4a/b and Glu298Asp, CYBA gene С242Т) in the development of recurrent miscarriage (RM) and preeclampsia. Subjects and methods. The investigation covered 93 patients with RM, 96 with preeclampsia, and 93 controlled women. Genotyping was carried out using a polymerase chain reaction. Results. In the women with RM, the allelic frequency of eNOS gene 4a and CYBA gene 242T was higher than that in the control group (p<0.05). Irrespective of age, RM was more common in female mutant allele carriers in terms of these two genes simultaneously (p=0.003). Among women less than 29 years old, RM was more frequently encountered in 4aa genotype carriers regardless the presence of the 242T mutant allele while the latter was an additional poor risk factor for RM in the 4aa genotype carriers (p=0.029). In the patients with preeclampsia, the frequency of the 802CC genotype of the AGT genotype was significantly lower than that in the control group (p<0.05), pointing to the protective effect of this mutation. The DD genotype of the ACE gene was more common in such patients. There were favorable combinations of the AGT and ACE genes (ii/TT, ii/ТС, ii/СС, iD/СС, DD/ СС) that were considerably less frequently detected in preeclampsia (p=0.019). The patients with moderate and severe preeclampsia were signif icantly more often found to have combinations of simultaneously the two polymorphic alleles of the ACE and CYBA genes (p=0.043). Conclusion. Maternal systemic disorders are an additional risk factor of pregnancy complications so patient genotyping to predict a risk for gestational complications should include the determination of polymorphisms mediating maternal systemic disorders.

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About the authors

O. N SADEKOVA

M.V. Lomonosov Moscow State University

Email: olniksa@gmail.com
Department of Biochemistry and Molecular Medicines, Faculty of Fundamental Medicine

I. P KNYAZEVA

M.V. Lomonosov Moscow State University

Department of Biochemistry and Molecular Medicines, Faculty of Fundamental Medicine

E. B YAROVAYA

M.V. Lomonosov Moscow State University

Department of Probability Theory, Faculty of Mechanics and Mathematics

V. E RADZINSKY

Peoples' Friendship University of Russia

Department of Obstetrics and Gynecology with a Course of Perinatology

E. M DEMIDOVA

OOO Clinic on Petrovka, Moscow

L. M SAMOKHODSKAYA

M.V. Lomonosov Moscow State University

Department of Biochemistry and Molecular Medicines, Faculty of Fundamental Medicine

V. A TKACHUK

M.V. Lomonosov Moscow State University

Department of Biochemistry and Molecular Medicines, Faculty of Fundamental Medicine

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