DISTRIBUTION OF THE GENOTYPES OF INTERLEULIN-1β, INTERLEUKIN-6, INTERLEUKIN-10, TUMOR NECROSIS FACTOR-α, VASCULAR ENDOTHELIAL GROWTH FACTOR IN WOMEN WITH POSTMENOPAUSAL OSTEOPOROSIS

Objective. To assess the role of genetic factors in osteoporosis and related fractures in postmenopausal women. Subjects and methods. A clinical genetic examination was performed in 413 women. Of them there were 375 persons who formed a control group of elderly healthy women without signs of osteoporosis and 38 osteoporotic women of the same age who had a menopausal length of more than 5 years. Seven polymorphisms in the promotor regions of the interleukin (IL) genes were investigated: TNF-α at positions С-863А, G-308A, G-238A, IL1ß T-31C, IL6 G-174C, IL10 C-592A, and VEGFA С-2578А. The polymorphisms examined were analyzed using the restriction assay of amplification products (restriction fragment length polymorphism analysis). Results. The distribution pattern of combinations of the genotypes of IL-1, IL-6, and IL-10, TNF-α at three positions and VEGFA among the elderly menopausal women with developed osteoporosis shows a number of significant distinctions from that in those with normal bone mineral density. The algorithm developed to calculate the mixed genetic signs including the combinations of all 7 study polymorphisms could reveal a number of highly significant associations with the development of osteoporosis. Conclusion. The high values of odds ratios and specificity of the obtained indicators are associated with the detection of a large number of genetic combinations among the women with postmenopausal osteoporosis, which are practically absent in the comparable group of menopausal women with normal bone mineral density.

TNF-α G-308A, TNF-α G-238A, IL-1ß T-31C, IL-6 G-174C, IL-10 C-592A, postmenopausal osteoporosis, cytokine gene polymorphism, TNF-α G-308A, TNF-α G-238A, IL1ß T-31C, IL6 G-174C, IL10 C-592A