CYTOKINE GENE POLYMORPHISM DURING TERM LABOR AND PROLONGED PREGNANCY


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Abstract

Objective. To study the role of cytokine gene polymorphism in term labor and prolonged pregnancy. Subject and methods. One hundred and eleven patients with term (n=87) or delayed (n=24) labor were examined. Polymerase chain reaction and melting curve analysis were used to study 10 cytokine gene polymorphic loci. Results. The women with term or delayed labor showed no significant differences in the allelic and genotyping distribution of the following polymorphisms: IL12: B -1188 C>A, IL18: -137 G>C, IL10: -592 А>C, VeGfA: 936 C>T, and -634 G>C, and IL1 gene family (IL1B: -31 T>C, IL1R1: -15858 C>T, and IL1RN: -390 T>C). The presence of -174 G>C IL-6 polymorphism was associated with the increased risk of delayed labor (OR = 4.9 (1.4 - 17.4); р = 0.004). The G -> A substitution at position -308 of the TNF gene was more common in the women who gave birth at term. The combination of IL-6-174 G>C and TNF-308G/G gene polymorphisms was associated with a 7.2-fold increase in the risk of prolonged pregnancy. Conclusion. The onset of labor at term is associated with the gene polymorphism of anti-inflammatory cytokines determining the level of their synthesis and secretion. 1) The presence of IL-6-174 G/C polymorphism in a woman is associated with a twofold increase in the incidence of delayed labor whereas the homozygous genotype G/G is typical of patients with term delivery. 2) The G->A substitution at position -308 in the promoter region of the TNF gene is a predisposing factor of prolonged pregnancy. 3) The risk of prolonged pregnancy increases by 7.2 times if there is a combination of IL-6 -174C and TNF -308G/G gene polymorphisms.

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About the authors

V. P RUMYANTSEVA

Acad. V.I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia

Email: valya-ru@yandex.ru

A. N STRIZHAKOV

I.N. Sechenov First Moscow State Medical University

Email: mmainter@mmascience.ru

O. R BAYEV

Acad. V.I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia

Email: metod_obsgyn@hotmail.com

A. E DONNIKOV

Acad. V.I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia

Email: a_donnikov@oparina4.ru

M. V RYBIN

I.N. Sechenov First Moscow State Medical University

Email: info@gkb7.mosgorzdrav.ru

G. T SUKHIKH

Acad. V.I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia

Email: g_sukhikh@oparina4.ru

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