ROLE OF PCR ASSAY IN THE DIAGNOSIS OF CONGENITAL AND NOSOCOMIAL INFECTIONS IN NEONATAL INFANTS


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Abstract

Background. Identification of an infectious agent is a key factor in determining the tactics of antibiotic therapy; however, the duration of a microbiological study leads to the delayed start of etiopathogenetic therapy and to the choice of a nonoptimal treatment regimen. The molecular genetic identification of microorganisms can yield a result with in a few hours. Objective. To define the role and clinical value of a real-time PCR assay in the diagnosis of congenital and nosocomial infections in neonatality. Subject and methods. The investigation compared microbiological and molecular genetic methods to identify microbial agents in a neonatal intensive care unit. Results. The high concordance of the used methods is shown on the basis of the results of 272 parallel studies. At the same time, PCR yielded results 4 times more promptly than pure culture identification during microbiological examination (preliminary cultures without antibiotic susceptibility) and 8 times more rapidly than the final results of microbiological examination being obtained. Conclusion. PCR is a highly accurate, rapid method that may be recommended for routine practice when concurrently used with conventional microbiological assay during infection control in neonatal units.

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About the authors

O. V IONOV

Academician V.I. Kulakov Research Center of Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia

Email: o_ionov@oparina4.ru

I. V NIKITINA

Academician V.I. Kulakov Research Center of Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia

Email: i_nikitina@oparina4.ru

O. V BURMENSKAYA

Academician V.I. Kulakov Research Center of Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia

Email: bourmenska@mail.ru

O. S NEPSHA

Academician V.I. Kulakov Research Center of Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia

Email: o_nepsha@oparina4.ru

D. Yu TROFIMOV

Academician V.I. Kulakov Research Center of Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia

Email: d_trofimov@oparina4.ru

A. E DONNIKOV

Academician V.I. Kulakov Research Center of Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia

Email: a_donnikov@oparina4.ru

S. D MITROKHIN

Academician V.I. Kulakov Research Center of Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia

Email: s_mitrokhin@mail.ru

T. V PRIPUTNEVICH

Academician V.I. Kulakov Research Center of Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia

Email: priput1@rambler.ru

L. A LYUBASOVSKAYA

Academician V.I. Kulakov Research Center of Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia

Email: l_lyubasovskaya@oparina4.ru

D. N DEGTYAREV

Academician V.I. Kulakov Research Center of Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia

Email: d_degtiarev@oparina4.ru

References

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