Diagnosis value of innate immunity in placental insufficiency


Cite item

Full Text

Open Access Open Access
Restricted Access Access granted
Restricted Access Subscription or Fee Access

Abstract

Objective. To elaborate criteria for predicting the development of intrauterine infection (IUI) in neonates on the basis of comprehensive clinical, immunological, and molecular genetic examinations of pregnant women with placental insufficiency (PI). Subject and methods. Two hundred and eighty-seven patients with PI underwent a prospective examination involving a study of the phagocytic activity of neutrophil granulocytes and determination of the serum levels of cytokines and the distribution of alleles and genotypes at polymorphic loci in the mothers and their newborn infants. Results. The inverse correlation of the high level of induced production of reactive oxygen species in the maternal venous blood and their low generation in the neonatal umbilical cord blood is a predictor of IUI. The latter more frequently develops in babies born to mothers with the T/T genotype of IL8-251 AT gene polymorphism (OR = 2.6 (12-5.8), p = 0.022), which allows the determination of this polymorphism to be used as a predictor for IUI. The A/A genotype of IL10-592 A>C IL-10 gene polymorphism in a baby is associated with the increased probability of IUI in PI, chronic hypoxia, andfetal growth retardation during pregnancy (OR = 18.9 (1.7-215.2), p = 0.031). Conclusion. The findings permit early genetic screening to be used both among pregnant women forming a group at high risk for PI and IUI and among their neonatal infants.

Full Text

Restricted Access

About the authors

Nataliya Anatolyevna Lomova

Academician V. I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia

Email: natasha-lomova@yandex.ru
junior scientific researcher of the obstetric Department

Nataliya Enkinovna Kan

Academician V. I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia

Email: n_kan@oparina4.ru
MD, head of the obstetric observation department

Lyudmila Viktorovna Vanko

Academician V. I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia

Email: lvanko@mail.ru
MD, professor, leading researcher of the laboratory of clinical immunology

Andrey Evgenyevich Donnikov

Academician V. I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia

Email: a_donnikov@oparina4.ru
candidate of medical Sciences, lecturer of faculty, senior scientific researcher laboratory of molecular genetics

Natalia Konstantinovna Matveeva

Academician V. I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia

Email: matveeva_nk@mail.ru
doctor of biology, senior scientific researcher

Anastasiya Sergeevna Belyaeva

Academician V. I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia

junior scientific researcher of the laboratory of clinical immunology

Nataliya Viktorovna Tyutyunnik

Academician V. I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia

Email: tysia07@bk.ru
resident

Gennadiy Tihonovich Sukhikh

Academician V. I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia

Email: g_sukhikh@oparina4.ru
MD, prof., academician of RAМS, CEO

References

  1. Crocker I.P, Baker P.N., Fletcher J. Neutrophil function in pregnancy and rheumatoid arthritis. Ann. Rheum. Dis. 2000; 59(7): 555-64.
  2. Vega-Sanchez R., Gomez-Lopez N., Flores-Pliego A., Clemente-Galvan S., Estrada-Gutierrez G., Zentella-Dehesa A. et al. Placental blood leukocytes are functional and phenotypically different than peripheral leukocytes during human labor. J. Reprod. Immunol. 2010; 84(1): 100-10.
  3. Hracsko Z, Orvos H., Novak Z., Pal A., Varga I.S. Evaluation of oxidative stress markers in neonates with intra-uterine growth retardation. Redox Rep. 2008; 13(1): 11-6.
  4. Liang C.C., Sun M.J., Lei H.Y., Chen S.H., Yu C.K., Liu C.C. et al. Human endothelial cell activation and apoptosis induced by enterovirus 71 infection. J. Med. Virol. 2004; 74(4): 597-603.
  5. Leviton A., Hecht J.L., Allred E.N., Yamamoto H., Fichorova R.N., Dammann O.; ELGAN Study Investigators. Persistence after birth of systemic inflammation associated with umbilical cord inflammation. J. Reprod. Immunol. 2011; 90(2): 235-43.
  6. Mestan K., Yu Y., Thorsen P., Skogstrand K., Matoba N., Liu X. et al. Cord blood biomarkers of the fetal inflammatory response. J. Matern. Fetal Neonatal Med. 2009; 22(5): 379-87.
  7. Madsen-Bouterse S.A.,Romero R., TarcaA.L.,Kusanovic J.P, Espinoza J.,Kim C.J. et al. The transcriptome of the fetal inflammatory response syndrome. Am. J. Reprod. Immunol. 2010; 63(1): 73-92.
  8. Simhan H.N., Mac Pherson T., Caritis S.N., Krohn M.A. Maternal and fetal Toll-like receptor 4 genotype and chorionic plate inflammatory lesions. Am. J. Obstet. Gynecol. 2008; 199(4): 400. e1-4.
  9. Verstraelen H., Verhelst R., Nuytinck L., Roelens K., De Meester E., De Vos D. et al. Gene polymorphisms of Toll-like and related recognition receptors in relation to the vaginal carriage of Gardnerella vaginalis and Atopobium vaginae. J. Reprod. Immunol. 2009; 79(2): 163-73.
  10. Verstraelen H., Verhelst R., Nuytinck L., Roelens K., De Meester E., De Vos D. et al. Correlation between placental histopathology and fetal/neonatal outcome: chorioamnionitis and funisitis are associated to intraventricular haemorrage and retinopathy of prematurity in preterm newborns. Gynecol. Endocrinol. 2011; 27(5): 319-23.
  11. Sacco G., Carmagnola D., Abati S., Luglio P.F., Ottolenghi L., Villa A. et al. Periodontal disease and preterm birth relationship: a review of the literature. Minerva Stomatol. 2008; 57(5): 233-46, 246-50.
  12. Vedmedovska N., Rezeberga D., Teibe U., Zodzika J., Donders G.G. Preventable maternal risk factors and association of genital infection with fetal growth restriction. Gynecol. Obstet. Invest. 2010; 70(4): 291-8.
  13. Bose C., Van Marter L.J., Laughon M., O’Shea T.M., Allred E.N., Karna P. et al.; Extremely Low Gestational Age Newborn Study Investigators. Fetal growth restriction and chronic lung disease among infants born before the 28th week of gestation. Pediatrics. 2009; 124(3): e450-8.
  14. Strunk T., Temming P., Gembruch U., Reiss I., Bucsky P., Schultz C. ifferential maturation of the innate immune response in human fetuses. Pediatr. Res. 2004; 56(2): 219-26.
  15. Ren X.Y., Xu L., Meng H.X., Zhao H.S., Lu R.F, Chen Z.B. et al. Family-based association analysis of S100A8 genetic polymorphisms with aggressive periodontitis. J. Periodontal Res. 2009; 44(2): 184-92.
  16. Schaaf B.M., Boehmke F., Esnaashari H., Seitzer U., Kothe H., Maass M. et al. Pneumococcal septic shock is associated with the interleukin-10-1082 gene promoter polymorphism. Am. J. Respir. Crit. Care Med. 2003; 168(4): 476-80.
  17. Schuurhof A., Janssen R., de Groot H., Hodemaekers H.M., de Klerk A., Kimpen J.L., Bont L. Local interleukin-10 production during respiratory syncytial virus bronchiolitis is associated with post-bronchiolitis wheeze. Respir. Res. 2011; 12: 121.
  18. Neta G.I., von Ehrenstein O.S., Goldman L.R., Lum K., Sundaram R., Andrews W., Zhang J. Umbilical cord serum cytokine levels and risks of small-for-gestational-age and preterm birth. Am. J. Epidemiol. 2010; 171(8): 859-67.
  19. Hahn-Zoric M., Hagberg H., Kjellmer I., Ellis J., Wennergren M., Hanson L.A. Aberrations in placental cytokine mRNA related to intrauterine growth retardation. Pediatr. Res. 2002; 51(2): 201-6.
  20. Vural P., Degirmencioglu S., Saral N.Y., Demirkan A., Akgul C., Yildirim G. et al. Tumor necrosis factor alpha, interleukin-6 and interleukin-10 polymorphisms in preeclampsia. J. Obstet. Gynaecol. Res. 2010; 36(1): 64-71.

Supplementary files

Supplementary Files
Action
1. JATS XML
Download

Copyright (c) 2014 Bionika Media

This website uses cookies

You consent to our cookies if you continue to use our website.

About Cookies