EFFICIENCY OF CERVICAL INTRAEPITHELIAL NEOPLASIA DIAGNOSIS USING A SET OF METHODS: LIQUID-BASED CYTOLOGY, P16/KI-67 DUAL IMMUNOSTAINING AND HPV TESTING


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Abstract

Objective. To evaluate the efficiency of p16/Ki-67 dual-staining in combination with liquid-based cytology and HPV testing in the detection of cervical intraepithelial neoplasia (CIN). Subject and methods. Cytology and biopsy specimens from 148 women with cervix uteri pathology were examined using liquid-based cytology, p16/Ki-67 dual immunostaining, and HPV testing. Results. The highest eff iciency of the cytological technique was revealed in cases of ≥ CIN2 (96.9% sensitivity and 100% specificity); that of HPV testing in < CIN2 (97.14% and 50.0%), and that of immunocytochemistry (ICC) in≥ CIN2 (98.48% and 100.0%). Conclusion. The effective use of all the three techniques to detect cervix uteri pathology (cytology, HPV testing, and ICC) enables one to solve the problem of not only the diagnosis of cervical precancer and cancer, but also their screening as much as possible.

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About the authors

Evgeniya Altarovna Kogan

Academician V.I. Kulakov Research Center of Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia

Email: koganevg@gmail.com
MD, PhD Professor, Head of the Pathology Department 117997, Russia, Moscow, Ac. Oparina str. 4

Nafisa Mynavarovna Fayzullina

Academician V.I. Kulakov Research Center of Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia

Email: nafisa05@inbox.ru
PhD, senior research associate of the Pathology Department 117997, Russia, Moscow, Ac. Oparina str. 4

Li Jiajing

I.M. Sechenov First Moscow State Medical University

Email: greenjing@qq.com
postgraduate student of anatomic pathology department 119991, Russia, Moscow, Trubetskaya str. 8 bld. 2

Tatiana Alexandrovna Demura

Academician V.I. Kulakov Research Center of Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia

Email: uursula90@gmail.com
MD, PhD, senior research associate of the Pathology Department 117997, Russia, Moscow, Ac. Oparina str. 4

Andrey Vladimirovich Kozachenko

Academician V.I. Kulakov Research Center of Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia

Email: a_kozachenko@oparina4.ru
MD, PhD, leading research associate of the Gynecological Department 117997, Russia, Moscow, Ac. Oparina str. 4

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