Immunohistochemical characteristics of endometrial receptivity in IVF cycles


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Abstract

Objective. To study the expression of leukemia inhibitory factor (LIF), transforming growth factor в 1 (TGF-fi), and vascular endothelial growth factor A (VEGF-A) in the surface and glandular epithelia and in the endometrial stromal component by an immunohistochemical (IHC) assay. Subject and methods. The present paper gives the IHC features of the expression of the examined factors in the endometrial tissue obtained in the implantation window in the cycle before in vitro fertilization (IVF) from 48 infertile women treated by IVF, followed by embryo transfer. The expression of the examined factors was compared in 23 women with an IVF pregnancy and in 25 without IVF pregnancy. Results. There was a markedly high expression of LIF in the surface and glandular epithelia and that of VEGF-A in the endometrial stroma of the women who had conceived versus those who had not (p < 0.01). The determination of the expression of LIF in the surface and glandular endometria and that of VEGF-A in the endometrial stroma serves to predict a 72.92% chance of conceiving in the IVF cycles. Conclusion. The optical density of expression of LIF in the surface and glandular epithelia and that of VEGF-A in the stroma of the epithelium obtained on days 20-23 of the cycle preceding the IVF cycle were the most informative indicators of IVF outcomes.

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About the authors

Dariko Aleksandrovna Niauri

St. Petersburg State University

Email: ofHce@inform.pu.ru
MD, Professor, Head of the Department of Obstetrics, Gynecology and Reproduction, Medical Faculty

Alexander Mkrtichevich Gzgzyan

D.O. Ott Research Institute of Obstetrics and Gynecology, North-Western Branch, Russian Academy of Medical Science; Saint Petersburg State University

Email: iagmail@ott.ru
MD, Head of Department Reproductive Technologies

Igor Moiseevich Kvetnoy

D.O. Ott Research Institute of Obstetrics and Gynecology, North-Western Branch, Russian Academy of Medical Science; Saint Petersburg State University

Email: igor.kvetnoy@yandex.ru
MD, professor, head of a Department patomorphology

Igor’ Yurievich Kogan

D.O. Ott Research Institute of Obstetrics and Gynecology, North-Western Branch, Russian Academy of Medical Science; Saint Petersburg State University

Email: iagmail@ott.ru
department of Physiology and Pathology of neonatal.

Liailia Kharryasovna Dzhemlikhanova

D.O. Ott Research Institute of Obstetrics and Gynecology, North-Western Branch, Russian Academy of Medical Science; Saint Petersburg State University

Email: office@inform.pu.ru
MD, Associate Professor of Obstetrics, Gynecology and Reproduction, Medical Faculty

Inna Otarovna Krikheli

D.O. Ott Research Institute of Obstetrics and Gynecology, North-Western Branch, Russian Academy of Medical Science; Saint Petersburg State University

Email: iagmail@ott.ru
MD, researcher

Irina Dmitrievna Fedorova

D.O. Ott Research Institute of Obstetrics and Gynecology, North-Western Branch, Russian Academy of Medical Science; Saint Petersburg State University

Email: iagmail@ott.ru
PhD, Senior Researcher, embryologist of Department Reproductive Technologies

Elena Aleksandrovna Lesik

D.O. Ott Research Institute of Obstetrics and Gynecology, North-Western Branch, Russian Academy of Medical Science; Saint Petersburg State University

Email: lesike@yandex.ru
PhD, Senior Researcher, embryologist

Yulia Najibovna Sharfi

St. Petersburg State University

Email: youlia03@yandex.ru
PhD student of the Department of Obstetrics, Gynecology and Reproduction, Medical Faculty

Julia Sergeevna Krylova

D.O. Ott Research Institute of Obstetrics and Gynecology, North-Western Branch, Russian Academy of Medical Science; Saint Petersburg State University

Email: emerald2008@mail.ru
Postdoctoral student of Department patomorphology

Evgeniia Mihailovna Shilnikova

St. Petersburg State University

Email: shilnikova-j@mail.ru
PhD student of the Department of genetics, Medical Faculty

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