Diagnostic value of assessing a peripheral blood lymphocyte phenotype in women with recurrent miscarriage


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Abstract

Objective. To compare the diagnostic value of peripheral blood lymphocyte phenotyping tests in women with recurrent miscarriage. Subjects and methods. The phenotype of lymphocytes was determined on 18-20 days of the menstrual cycle in 70 patients with recurrent miscarriage and in 15 fertile women. Results. The count of the cells with the CD4 +CD25 highCD127 low/-phenotype was significantly lower and that with the CD3 -CD8 +, CD56 +, CD3 +CD56,16 +, CD3 -CD56,16 +, SCD56,16 +, CD200 +phenotype and the ratio of cells with the CD200 + and CD4 +CD25 highCD127 low/-phenotypes were higher in the women with recurrent miscarriage. The sensitivity and specificity of the tests for these indicators were estimated. Conclusion. The tests for the relative and absolute counts of regulatory T cells (T reg) and the test for the ratio of the relative count of cells with the CD200 + phenotype and T reg have the best characteristics of diagnostic value: an equally high specificity (92.9%) and a high sensitivity (86.6, 83.3, and 90.9%, respectively). Further investigations are to study the value of these tests as markers for the efficiency of performed immunotherapy for recurrent miscarriage.

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About the authors

Lyubov V. Krechetova

Academician V. I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia

Email: k_l_v_@mail.ru
Ph.D. in medical sciences, Head of Laboratory of Clinical Immunology

Nana K. Tetruashvili

Academician V. I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia

Email: n_tetruashvili@oparina4.ru
Doctor of Medicine, Head of the Department of Pregnancy Loss Prevention and Therapy

Nelly A. Khachatryan

Academician V. I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia

Email: nelly1986@mail.ru
Graduate student, Department of Pregnancy Loss Prevention and Therapy

Valentina V. Vtorushina

Academician V. I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia

Email: vtorushina@inbox.ru
Ph.D. in medical sciences, doctor of laboratory diagnostics in Laboratory of Clinical Immunology

Elena Olegovna Stepanova

Academician V. I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia

Email: elena2404.07@mail.ru
Junior researcher, Laboratory of Clinical Immunology

Marina A. Nikolaeva

Academician V. I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia

Email: nikolaeva_ma@mail.ru
Doctor of Science, Leading researcher, Laboratory of Clinical Immunology

Gennady T. Sukhikh

Academician V. I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia

Email: g_sukhikh@oparina4.ru
MD, PhD, Professor, Academician of Russian Academy of Sciences, Director

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