FREE EMBRYONIC DNA LEVELS IN THREATENED RECURRENT MISCARRIAGE AND UNCOMPLICATED PREGNANCY AT LESS THAN 22 WEEKS' GESTATION


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Abstract

Objective. To make a comparative analysis of the peripheral blood levels of free embryonic DNA (fe-DNA) in women with threatened recurrent miscarriage and healthy pregnant women at 6 to 22 weeks’ gestation. Subjects and methods. A prospective cohort study was conducted in 57 pregnant women, including 33 women with recurrent miscarriage and 24 with an uncompromised obstetric and gynecological history. Regardless of fetal gender, fe-DNA was determined by a real-time polymerase chain reaction assay at a 2-week interval. Results. The clinical manifestations of threatened and incipient abortion were found to be related to high fe-DNA levels without regard to a patient’s history. In the women with recurrent miscarriage and an uncomplicated course of the first half of pregnancy, the levels of fe-DNA were established to be significantly higher than those in healthy pregnant women, which may predispose to placenta-related gestational complications. Conclusion. This data should be further analyzed to clarify the role of these changes in the implementation of the later gestational complications and possibilities of their use in clinical practice.

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About the authors

Nane G. Parsadanyan

Academician V. I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia

Email: nnnpars@mail.ru
Graduate student, Department of Pregnancy Loss Prevention and Therapy Moscow 117997, Ac. Oparina str. 4, Russia

Ekaterina S. Shubina

Academician V. I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia

Email: e_shubina@oparina4.ru
Graduate student, laboratory of molecular genetic methods Moscow 117997, Ac. Oparina str. 4, Russia

Nana K. Tetruashvili

Academician V. I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia

Email: tetrauly@mail.ru
MD, Head of the Department of Pregnancy Loss Prevention and Therapy Moscow 117997, Ac. Oparina str. 4, Russia

Dmitrii Y. Trofimov

Academician V. I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia

Email: d_troflmov@oparina4.ru
Ph.D. in biological sciences, Head of Laboratory of molecular genetic methods Moscow 117997, Ac. Oparina str. 4, Russia

Gennady T. Sukhikh

Academician V. I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia

Email: gtsukhikh@mail.ru
MD, Professor, Academician of Russian Academy of Medical Sciences, Director Moscow 117997, Ac. Oparina str. 4, Russia

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