INNATE IMMUNITY SIGNALING RECEPTORS IN THE INDUCTION OF APOPTOSIS IN EARLY MISCARRIAGE


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Abstract

Objective. To study the specific features of expression of toll-like receptor (TLR) 1-10 mRNA and the major apoptotic enzyme caspase-3a in patients with recurrent miscarriage at 6-10 weeks’ gestation. Subject and methods. The decidual tissue obtained when curetting the uterine in 57patients with spontaneous miscarriage at 6-10 weeks’ gestation (a study group) and in 57patients who had undergone medical abortion in the same gestational period (a control group) were examined. The expression of TLR 1-10 mRNA and caspase-3a in the endometrium was investigated using a quantitative polymerase chain reaction (PCR). Results. The patients with early miscarriage of infectious and inflammatory genesis showed an increased endometrial expression of TLR3, the ligand of which was viral double-stranded RNA. Those with spontaneous miscarriage were noted to have a decrease in TLR6 expression, which was strongly correlated with the endometrial activation of caspase-3a that is a major enzyme of irreversible apoptosis. There was no correlation between the expression of caspase and other TLRs. The expression of TLR6 had a positive moderate correlation with the number of births (R = 0.32; p = 0.03) and a weak negative correlation with the number of spontaneous miscarriages (R = -0.29; p = 0.002) in the history. Conclusion. Thus, the normal expression of TLR6 may play a protective role in the endometrium, interfering with miscarriages due to the prevention of apoptosis.

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About the authors

Olga Petrovna Lebedeva

Belgorod State National Research University

Email: safonova2@yandex.ru
Ph.D. Candidate, associate professor of the Department of Obstetrics and Gynecology Belgorod 308015, Pobedy str. 85, Russia

Olesya Nikolaevna Ivashova

Belgorod State National Research University

Email: pachomw@yandex.ru
postgraduate student of the Department of Obstetrics and Gynaecology Belgorod 308015, Pobedy str. 85, Russia

Natalia Yurevna Starceva

Belgorod State National Research University

Email: ivashovao2@yandex.ru
postgraduate student of the Department of Obstetrics and Gynaecology Belgorod 308015, Pobedy str. 85, Russia

Sergey Petrovich Pakhomov

Belgorod State National Research University

Email: starceva_nataliya@mail.ru
PhD Doctor, Professor, Head of the Department of Obstetrics and Gynaecology Belgorod 308015, Pobedy str. 85, Russia

Mikhail Ivanovish Churnosov

Belgorod State National Research University

Email: churnosov@bsu.edu.ru
PhD Doctor, Professor, Head of the Department of Medical Biology Belgorod 308015, Pobedy str. 85, Russia

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