DIAGNOSTIC ROLE OF THE CLINICAL AND MOLECULAR GENETIC PREDICTORS OF INTRAUTERINE INFECTION


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Abstract

Objective. To create a model for predicting intrauterine infection (IUI) in neonatal infants on the basis of a study of an association between clinical and molecular genetic factors. Subjects and methods. A total of357pregnant women underwent a prospective examination involving the analysis of their clinical characteristics and the study of allele and genotype distribution in the cytokine gene polymorphic loci. Results. A relationship was established between the maternal carriage of the IL1B -31T allele and the presence of IUI (p = 0.002). The mothers also showed a statistically significant association of the IL6-174 C/Gpolymorphism (p = 0.02) and the IL8 T/T-251 A>T polymorphism (p = 0.04) with the presence of IUI. Multivariate analysis of the clinical, anamnestic, and molecular genetic data has yielded a mathematical model that may predict the development of IUI. The model includes the genotypes at the IL8 -251 A>T and IL1B -31 T>C loci, hydramnios in the second trimester, threatened preterm delivery, intrauterine growth restriction, and/ or placental insufficiency in the third trimester. The sensitivity of the model was 60% and its specificity was 79%. Conclusion. The findings allow the found clinical and molecular genetic factors to be used as predictors of intrauterine growth restriction in pregnant women.

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About the authors

Natalia E. Kan

Academician V.I. Kulakov Research Center of Obstetrics, Gynecology and Perinatology Ministry of Health of Russia

Email: n_kan@oparina4.ru
PhD, MD, the chief physician 117997, Russia, Moscow, Ac. Oparina str. 4

Elena A. Sirotkina

Academician V.I. Kulakov Research Center of Obstetrics, Gynecology and Perinatology Ministry of Health of Russia

Email: e_sirotkina@oparina4.ru
the postgraduate student 117997, Russia, Moscow, Ac. Oparina str. 4

Victor L. Tyutyunnik

Academician V.I. Kulakov Research Center of Obstetrics, Gynecology and Perinatology Ministry of Health of Russia

Email: v_tioutiounnik@oparina4.ru
PhD, MD, the head of the obstetric observation department 117997, Russia, Moscow, Ac. Oparina str. 4

Andrey E. Donnikov

Academician V.I. Kulakov Research Center of Obstetrics, Gynecology and Perinatology Ministry of Health of Russia

Email: a_donnikov@oparina4.ru
PhD, senior scientific researcher laboratory of molecular genetics 117997, Russia, Moscow, Ac. Oparina str. 4

Andrey A. Bystritskiy

Academician V.I. Kulakov Research Center of Obstetrics, Gynecology and Perinatology Ministry of Health of Russia

Email: andrey.bystritskiy@yandex.ru
PhD, leader researcher laboratory of molecular genetics 117997, Russia, Moscow, Ac. Oparina str. 4

Vladslava V. Kadochnikova

Academician V.I. Kulakov Research Center of Obstetrics, Gynecology and Perinatology Ministry of Health of Russia

Email: Vladavi@mail.ru
PhD, Senior scientific researcher, Laboratory of Human Histocompability Genetics 117997, Russia, Moscow, Ac. Oparina str. 4

Elena G. Markelova

State Research Center, Institute of Immunology, Federal Biomedical Agency of Russia

Email: markelova@dna-technology.ru
PhD Senior scientific researcher, Laboratory of Human Histocompability Genetics 115478, Russia, Moscow, Kashirskoye sh. 24 bld. 2

Tatiana A. Kurchakova

Academician V.I. Kulakov Research Center of Obstetrics, Gynecology and Perinatology Ministry of Health of Russia

Email: t_kurchakova@oparina4.ru
postgraduate student 117997, Russia, Moscow, Ac. Oparina str. 4

Aleksandra A. Veresova

Academician V.I. Kulakov Research Center of Obstetrics, Gynecology and Perinatology Ministry of Health of Russia

Email: a_veresova@oparina4.ru
postgraduate student 117997, Russia, Moscow, Ac. Oparina str. 4

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