POSSIBILITY FOR THE PREDICTION AND EARLY DIAGNOSIS OF PREECLAMPSIA FROM THE URINARY PEPTIDE PROFILE


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Abstract

Objective. To determine the possibility of diagnosing preeclampsia from the urinary peptide profile. Subject and methods. To identify candidate peptide biomarkers for preeclampsia, a case-control study was performed in women with verified moderate to severe preeclampsia. Eight urine samples were examined in each woman with moderate to severe preeclampsia and in each control woman with physiological pregnancy. The peptide fraction was analyzed using first a MALDI-TOF mass spectrometer and then a Dionex 300 nanoflow chromatographer (USA) and a Maxis time-of-flight mass spectrometer (Bruker, Bremen, Germany). The findings were processed by bioinformatics techniques. Results. This investigation has revealed both some previously known peptide biomarkers of preeclampsia and a number of novel endogenous urinary peptides specific for severe preeclampsia. Out of them, a subgroup of 6 peptides, fragments of the α-chain of fibrinogen and collagens (types I and III), as well as uromodulin, which allow determination of the development of severe preeclampsia with 100% diagnostic value, may be identified. Conclusion. The investigation has shown the high diagnostic potential of a precision analysis of endogenous peptides in the urine of pregnant women, by applying chromatography mass spectrometry. The verification of the found marker peptides calls for investigations on a larger patient sample and for inclusion of additional comparison groups to estimate the specificity of a panel of peptides.

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About the authors

Natalia L. Starodubtseva

Academician V.I. Kulakov Research Center of Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia; Institute for Energy Problems of Chemical Physics, Russian Academy of Sciences

Email: n_starodubtseva@oparina4.ru
PhD, Head of Proteomics Laboratory of Human Reproduction; Researcher, Laboratory of ionic and molecular physics 117997, Russia, Moscow, Ac. Oparina str. 4

Anna E. Bugrova

Academician V.I. Kulakov Research Center of Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia; N.M. Emanuel Institute of Biochemical Physics, Russian Academy of Sciences

Email: anna.bugrova@gmail.com
PhD, Senior Scientist Proteomics Laboratory of Human Reproduction; Senior Researcher, Neurochemistry Laboratory

Alexey S. Kononikhin

Academician V.I. Kulakov Research Center of Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia; Institute for Energy Problems of Chemical Physics

Email: konoleha@yandex.ru
PhD, MN, Researcher, Laboratory of Proteomics of Human Reproduction; Senior Researcher Laboratory ionic and molecular physics 117997, Russia, Moscow, Ac. Oparina str. 4

Olga Vladimirovna Vavina

Academician V.I. Kulakov Research Center of Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia

Email: o_vavina@oparina4.ru
obstetrician-gynecologist maternity ward 117997, Russia, Moscow, Ac. Oparina str. 4

Victoria A. Shirokova

Institute for Energy Problems of Chemical Physics, Russian Academy of Sciences

Email: vik4192@rambler.ru
Student of the laboratory of ionic and molecular physics 119334, Russia, Moscow, Leninsky pr-t 38 bld. 2

Vladimir Aleksandrovich Naumov

Academician V.I. Kulakov Research Center of Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia

Email: looongdog@gmail.com
researcher at the Laboratory of Bioinformatics 117997, Russia, Moscow, Ac. Oparina str. 4

Irina A. Garanina

Academician V.I. Kulakov Research Center of Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia

Email: irinagaranina24@gmail.com
student of bioinformatics lab 117997, Russia, Moscow, Ac. Oparina str. 4

Vadim V. Lagutin

Academician V.I. Kulakov Research Center of Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia

Email: laggi@mail.ru
researcher at the Laboratory of Bioinformatics 117997, Russia, Moscow, Ac. Oparina str. 4

Igor A. Popov

N.M. Emanuel Institute of Biochemical Physics, Russian Academy of Sciences; Academician V.I. Kulakov Research Center of Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia

Email: hexapole@gmail.com
PhD Cand., Senior Researcher, Laboratory of mass spectrometry of biomacromolecules; Senior Researcher 119934, Russia, Moscow, Kosygina str. 4

Natalia Sergeevna Loginova

Academician V.I. Kulakov Research Center of Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia

Email: n_loginova@oparina4.ru
PhD, physician assistant, laboratory of Clinical Immunology 117997, Russia, Moscow, Ac. Oparina str. 4

Zulfiya S. Khodjaeva

Academician V.I. Kulakov Research Center of Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia

Email: z_khodzhaeva@oparina4.ru
MD, professor, main researcher of the 1st obstetric department of pathology of pregnancy 117997, Russia, Moscow, Ac. Oparina str. 4

Vladimir Evgen'evich Frankiewicz

Academician V.I. Kulakov Research Center of Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia

Email: v_frankevich@oparina4.ru
PhD Cand., Head of Systems Biology in reproduction 117997, Russia, Moscow, Ac. Oparina str. 4

Evgeniy N. Nikolaev

N.M. Emanuel Institute of Biochemical Physics, Russian Academy of Sciences;Institute for Energy Problems of Chemical Physics, Russian Academy of Sciences

Email: ennikolaev@rambler.ru
doctor of sciences, Head of the Laboratory of mass spectrometry of biomacromolecules, ; Head of the Laboratory of ion and molecular physics 119934, Russia, Moscow, Kosygina str. 4

Gennady T. Sukhikh

Academician V.I. Kulakov Research Center of Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia

Email: g_sukhikh@oparina4.ru
Ph.D., Academician, Professor, Director 117997, Russia, Moscow, Ac. Oparina str. 4

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