Impact of intravenous laser blood irradiation on the morphofunctional status of platelets in puerperas after abdominal delivery


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Abstract

Objective. To evaluate the impact of intravenous laser blood irradiation (ILBI) as a technique to prevent IICs on the morphofunctional parameters of platelets in puerperas after cesarean section. Subjects and methods. A total of 126 postcesarean section puerperas who received antibiotic prophylaxis with intravenous amoxiclav 1.2 g 15 minutes before skin incision were examined. On day 1 postsurgery, Group 1 (a study group) (n = 65) had daily 15-minute ILBI using a Solaris apparatus (Russia) with a helium-neon laser irradiation at 0.63 nm for 5 days. Group 2 (a comparison group) (n = 61) had antibiotic prophylaxis only. On days 1 and 6, the 126 puerperas underwent peripheral blood tests: platelet counts, blood coagulation tests with thromboelastography and platelet aggregation, and real-time monitoring of the morphofunctional status of platelets by coherent phase microscopy (CPM) using a Bioni-CPM module of a hardware and software complex (Westtrade, Russia) for clinical and laboratory diagnosis with digital image processing. Results. The mean age of Groups 1 and 2 puerperas was 31.2±5.4 and 30.5±5.1 years, respectively (р ?? 0.05). According to their somatic and obstetric/gynecological status, all the puerperas belonged to an IIC risk group. The investigation established that ILBI had an integral modifying effect on the functional activity of circulating platelets. ILBI in the puerperas contributed to the increased counts of resting platelets and the decreased level of active platelets, leading to suppression of the total adhesive and aggregation ability of platelets. After ILBI, the mean values of platelet diameter, perimeter, and area were slightly higher than the control ones, which might be due to the fact that there were young platelet subpopulations having high morphometric values, but a normal functional status in the bed. ILBI lowered the blood coagulation potential, as witnessed by decreases in fibrinogen concentrations, thrombodynamic potential index, and intravascular coagulation, and a prolongation of the r+k index, which favored better microcirculation and tissue oxygenation. Conclusion. The investigation has shown that postoperative ILBI is efficient and safe in IIC risk group puerperas, by affecting platelet morphofunctional parameters and hemostatic system, which allows ILBI to be recommended as part of measures to prevent IICs after cesarean section.

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About the authors

T. A Fedorova

Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia

Email: t_fyodorova@oparina4.ru
h.D., MD., Head of transfusion department

I. A Vasilenko

Maimonides State Classical Academy, Ministry of Education and Science of Russia; Russian Medical Academy of Postgraduate Education, Ministry of Health of Russia

Email: vasilenko0604@gmail.com
Ph.D., MD., Head of Department of Biology of Maimonides State Classical Academy; Head of medical cytology laboratory of Russian Medical Academy of Postgraduate Education

T. K Puchko

Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia

Email: t_puchko@oparina4.ru
Ph.MD., the leading scientific researcher of delivery department

K. G Bykova

Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia

Email: k_bykova@oparina4.ru
obstetrician-gynecologist of postdelivery department

V. B Metelin

Maimonides State Classical Academy, Ministry of Education and Science of Russia; Russian Medical Academy of Postgraduate Education, Ministry of Health of Russia

Email: verrv@rambler.ru
Ph.D., associate professor of Department of Biology of Maimonides State Classical Academy; senior researcher of medical cytology laboratory of Russian Medical Academy of Postgraduate Education.

A. V Matveeva

Russian Medical Academy of Postgraduate Education, Ministry of Health of Russia

Email: svia@rambler.ru
research associate of medical cytology laboratory

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