ROLE OF TOLL-LIKE RECEPTORS 2, 4, 9 IN THE ENDOMETRIUM IN CHRONIC ENDOMETRITIS


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Abstract

Objective. To study the expression of Toll-like receptors (TLRs) 2, 4, 9 in the endometrium in chronic endometritis (CE). Subject and methods. The investigation included 60 reproductive-aged patients (mean age 32.1±5.2 years). All the patients underwent morphological and microbiological examinations of endometrial biopsy specimens. The expression of TLRs 2, 4, 9 was investigated by immunohistochemistry (IHC) and real-time reverse transcription PCR (RT-PCR). According to the endometrial morphological findings, the patients were divided into 2 groups: 1) 45 women with morphologically verified CE; 2) 2) 15 apparently healthy women (proliferative-phase endometrium). Mann-Whitney U-test and Spearman’s correlation test were applied for statistical processing. The values of p < 0.05 were considered statistically significant for all the tests. Results. Group 1 showed a significantly increase in the expression of TLRs in the endometrium as compared to Group 2 (p < 0.05). Comparative analysis revealed no statistical difference in TLR expression in the women with CE in the presence and absence of infective agents in their endometrial biopsy specimens. There were weak direct correlations: TLR2 expression between the endometrial epithelial cells by IHC and the endometrium as a whole by RT-PCR (r = 0.3; p = 0.024); TLR9 expression between endometrial stromal cells, vessels by IHC and the endometrium as a whole by RT-PCR (r = 0.3;p = 0.028; r = 0.3;p = 0.023, respectively). Conclusion. Examination of the expression of TLRs 2, 4, 9 in the endometrium could specify that to prevent the chronization of an endometrial inflammatory process, it was necessary to rapidly eliminate the pathogen, to prevent its persistence, to restore the normal architectonics of the uterine mucosa, and to suppress TLR overexpression predisposing to the creation of the vicious pathogenetic circle.

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About the authors

Evgenia A. Kogan

Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia

Email: koganevg@gmail.com; e_kogan@oparina4.ru
MD, Professor, Head of the 1st patology department Moscow 117997, Ac. Oparina str. 4, Russia

Natalia A. Gombolevskaya

Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia

Email: gombolevskaya@yandex.ru
a graduate student Moscow 117997, Ac. Oparina str. 4, Russia

Tatiana A. Demura

Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia

Email: uursula90@gmail.com; t_demura@oparina4.ru
Ph.D., Senior Researcher of the 1st pathology department Moscow 117997, Ac. Oparina str. 4, Russia

Larisa A. Marchenko

Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia

Email: l.a.marchenko@yandex.ru
MD, professor, leading researcher of gynecological endocrinology department Moscow 117997, Ac. Oparina str. 4, Russia

Olga Vladimirovna Burmenskaya

Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia

Email: bourmenska@mail.ru
PhD, researcher at the Laboratory of molecular genetic methods Moscow 117997, Ac. Oparina str. 4, Russia

Nafisa Munavarovna Fayzullina

Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia

Email: naflsa.fayzullina@gmail.com
Ph.D., Senior Researcher of the 1st pathology department Moscow 117997, Ac. Oparina str. 4, Russia

Vera V. Muraveva

Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia

Email: v_muravieva@oparina4.ru
PhD, Senior Scientist of Microbiology Laboratory Moscow 117997, Ac. Oparina str. 4, Russia

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