Implementation of assisted reproductive technology programs in patients with smooth endoplasmic reticulum aggregates in the cytoplasm of oocytes
- Authors: Syrkasheva A.G.1, Kazakova V.V.2, Dolgushina N.V.1, Romanov A.Y.2, Andreeva M.G.1, Yarotskaya E.L.1
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Affiliations:
- Research Center of Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia
- M.Yu. Lomonosov Moscow State University
- Issue: No 7 (2016)
- Pages: 54-59
- Section: Articles
- URL: https://journals.eco-vector.com/0300-9092/article/view/247713
- DOI: https://doi.org/10.18565/aig.2016.7.54-59
- ID: 247713
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Abstract
The quality of oocytes and their morphology have a great impact on the outcomes of assisted reproductive technology (ART) programs. The literature contains contradictory data on the impact of abnormal smooth endoplasmic reticulum (SER) aggregates on the parameters of embryogenesis, the occurrence of pregnancy and its course. Objective. To reveal risk factors for SER aggregates in the cytoplasm of oocytes and to evaluate their impact on the embryological characteristics and clinical outcomes of ART programs. Subjects and methods. The prospective cohort study included 162 women treated with in vitro fertilization/ intracytoplasmic sperm injection (IVF/ICSI) for infertility. The patients were divided into 2 groups: 1) women with 100% oocytes with SER aggregates; 2) those with 100% morphologically normal oocytes. The oocytes were assessed by light microscopy during fertilization with ICSI. Results. A history of recurrent genital herpes and Chlamydia infection increased a risk for the appearance of SER aggregates in the cytoplasm of oocytes in the patients receiving ART programs by 3.7 times. When a patient received more than 10 mature oocytes, her chances of having oocytes with SER aggregates were 3.3 times higher. Other clinical anamnestic parameters, as well as the specific features of superovulation did not affect the occurrence of this abnormality. The rate of oocyte fertilization and the quality of obtained embryos were lower in the SER aggregate group. In the women with SER aggregates, their clinical pregnancy occurred 4.1 times less frequently and the rate of early reproductive losses was 3.86 times higher than in those with morphologically normal oocytes. Conclusion. The performed study provided evidence for the substantial negative effect of SER aggregates on the outcomes of ART programs and revealed risk factors for this pathology. There is no question that this problem needs further investigation, a search for the causes and mechanisms of this oocyte dysmorphism, and determination of the optimal management tactics for patients with abnormal oocytes.
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About the authors
Anastasia Grigorievna Syrkasheva
Research Center of Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia
Email: anast.syrkasheva@gmail.com
M.D., Researcher of R&D Department
Vera Vladimirovna Kazakova
M.Yu. Lomonosov Moscow State University
Email: verakazakova@gmail.com
undergraduate of the faculty of fundamental medicine
Nataliya Vitalievna Dolgushina
Research Center of Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia
Email: n_dolgushina@oparina4.ru
M.D., Ph.D., M.P.H., Head of R&D Department
Andrey Yurievich Romanov
M.Yu. Lomonosov Moscow State University
Email: romanov1553@yandex.ru
undergraduate of the faculty of fundamental medicine
Maria Gennadievna Andreeva
Research Center of Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia
Email: onischenkomaria@mail.ru
M.D., Postgraduate of the Department of assistive reproductive technology in the treatment of infertility
Ekaterina Lvovna Yarotskaya
Research Center of Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia
Email: e_yarotskaya@oparina4.ru
M.D., Ph.D., Head of the Department for International Cooperation
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