Experimental rationale for the use of cell technologies for the correction of myometrial scar


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Abstract

Objective. To investigate changes in the tissues of a scar and uterus in rats and to establish whether there may be spontaneous labor after ligation of the uterine horns, followed by the administration of multicomponent mesenchymal stromal cells ((MSCs) into these areas in order to prove the principal possibility of improving scar repair and the feasibility of reducing the rate of cesarean section in patients with the uterus operated on. Material and methods. The investigators determined whether pregnancy and delivery might occur in 66 laboratory Wistar rats with a myometrial scar and also explored tissue changes after use of multicomponent MSCs with the GFP transgene when simulating hydrometra in 170 inbred Wag rats. Results. After injecting multicomponent MSCs into the uterine scar, the latter showed a larger number of de novo formed vessels with the participation of the administered cells. Multicomponent MSCs were used to construct entire vascular walls or their individual elements. After application of multicomponent MSCs, the animals began to parturiate by 2 estrous cycles earlier; the proportion of parturient rats in this group was higher; they had more both a total number of broods and the maximal number of infant rats than the animals that had not received cell technologies. The animal mortality after use of multicomponent MSCs was, on the contrary, lower. Conclusion. Following administration of muticomponent MSCs, there is a clear trend towards acceleration of reparative processes in the rat uterus with a change in its scar.

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About the authors

Oleg G. Pekarev

Research Center of Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia

Email: o_pekarev@oparina4.ru
MD, Professor, Deputy chief physician

Igor V. Maiborodin

Institute of Chemical Biology and Fundamental Medicine, Siberian Branch, Russian Academy of Sciences

Email: imai@mail.ru
MD, Professor, leading researcher laboratory of stem cell

Ivan M. Pozdnyakov

Novosibirsk State Medical University

Email: rdngpc@ngs.ru
MD, Professor of the Department of the obstetrics and gynecology

Natalia V. Onoprienko

Novosibirsk State Medical University

Email: nata.onoprienko@gmail.com
candidate of medical sciences, assistant of the chair Department of the obstetrics and gynecology

Evgeniya O. Pekareva

Novosibirsk City Perinatal Center

Email: pekareva_e@mail.ru
MD, candidate of medical sciences

Anatoliy A. Anikeev

Institute of Chemical Biology and Fundamental Medicine, Siberian Branch, Russian Academy of Sciences

Email: imai@mail.ru
MD, candidate of medical sciences, senior researcher laboratory of stem cell

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