DIRECT MASS SPECTROMETRY INVESTIGATION OF ENDOMETRIOTIC FOCI AT VARIOUS SITES


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Abstract

Objective. To create an approach to analyzing differences in the molecular composition of the eutopic and ectopic endometrium at various sites, by using direct mass spectrometry. Subjects and methods. The case-control study included 30 patients with ovarian endometrioid cysts and peritoneal endometriosis (Grades III-IVendometriosis according to the classification of the American Fertility Society). The diagnosis of external genital endometriosis was verified during clinical and instrumental examination, including diagnostic and therapeutic laparoscopy, and ultimately confirmed by histology. The endometrium was sampled in all the patients during hysteroscopy. Each sample was divided into two parts: for pathological studies and for direct mass spectrometry (EESI-based procedures, liquid extraction of low molecular weight substances from tissue with simultaneous ionization). The characteristic high- and low-resolution mass spectra of lipid tissue were obtained. Results. The ectopic endometrium, unlike the eutopic one, showed significantly varying levels of 5 different lipid classes: phosphatidylcholines (PC 32:1, PC 0-36:3, PC 38:7, PC 38:6, PC 40:7, PC 40:6, PC O-42:1), phosphoethanolamine (PE O-20:0), sphingomyelin (SM 34:1), diglyceride (DG 44:9), and triglycerides (TG 41:2, TG 49:4, TG 52:3). Phosphatidylcholines and sphingomyelin may be proposed as possible biomarkers for endometriosis since these lipids are closely linked to apoptotic suppression, oxidative stress, and cell malignancy. Conclusion. Lipidomic analysis of endometrioid tissues allows the identification of new molecular processes that cause the enhanced cell growth characteristic for the aggressive form of endometriosis. Not only new diagnostic approaches, but also potential therapeutic targets and novel treatments aimed at pathophysiological mechanisms may be proposed as a result of validation.

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About the authors

Anna Valerevna Borisova

Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia

Email: aneta_b@mail.ru
PhD student Moscow 117997, Ac. Oparina str. 4, Russia

Nataliia Leonidovna Starodubtseva

Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia; Moscow Institute of Physics and Technology

Email: n_starodubtseva@oparina4.ru
PhD, Head of Laboratory of Proteomics of Human Reproduction Moscow 117997, Ac. Oparina str. 4, Russia; Dolgoprudny 141700, Institutskiy per. 9, Moscow Region, Russia

Andrey Vladimirovich Kozachenko

Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia

Email: andreykozachenko@list.ru
MD, PhD, Senior Research Fellow Moscow 117997, Ac. Oparina str. 4, Russia

Vitaliy Viktorovich Chagovets

Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia

Email: vvchagovets@gmail.com
PhD, Senior Researcher of Proteomics of Human Reproduction Moscow 117997, Ac. Oparina str. 4, Russia

Dinara Failevna Salimova

I.M. Sechenov First Moscow State Medical University, Ministry of Health of Russia

Email: Salimova.1993@mail.ru
Student Moscow 119991, Malaya Pirogovskaya str. 8/21, Russia

Alexey Sergeevich Kononikhin

Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia; Moscow Institute of Physics and Technology

Email: a_kononihin@oparina4.ru
PhD, Researcher of Proteomics of Human Reproduction Moscow 117997, Ac. Oparina str. 4, Russia; Dolgoprudny 141700, Institutskiy per. 9, Moscow Region, Russia

Evgeniya Altarovna Kogan

I.M. Sechenov First Moscow State Medical University, Ministry of Health of Russia

MD, professor of Academician A.I. Strukov Pathological Anatomy Department Moscow 119991, Malaya Pirogovskaya str. 8/21, Russia

Leila Vladimirovna Adamyan

Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia

Email: adamyanleila@gmail.com
Academician of the Russian Academy of Sciences, Professor, Honoured Scientist of the Russian Federation, Deputy Director for Science Moscow 117997, Ac. Oparina str. 4, Russia

Vladimir Evgenievich Frankevich

Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia

Email: v_frankevich@oparina4.ru
PhD, Head of Department of Systems Biology in Reproduction Moscow 117997, Ac. Oparina str. 4, Russia

Gennady T. Sukhikh

Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia

Email: gtsukhikh@mail.ru
MD, PhD, Professor, Academician of Russian Academy of Sciences, Director Moscow 117997, Ac. Oparina str. 4, Russia

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