QUANTIFICATION OF PLASMA MICRORNA EXPRESSION IN PREGNANT WOMEN WITH PREECLAMPSIA


Cite item

Full Text

Open Access Open Access
Restricted Access Access granted
Restricted Access Subscription or Fee Access

Abstract

Objective. To study the expression of plasma microRNAs (has-mir-29b-3P, has-mir-34a-5p, has-mir-141-3p, and miR-376c) in preeclampsia (PE). Material and methods. 36 pregnant women were examined, by analyzing their clinical characteristics and investigating the expression of plasma microRNA. Results. There was a significant increase in the expression of three microRNAs: has-mir-29b-3p, has-mir-34a-5p, and has-mir-141-3p in the plasma of pregnant women with PE as compared to the control group. There were differences in the expression of miR-376c, but there was a high degree of variation in the expression level in the groups. The highest diagnostic accuracy ( 76.7%) was achieved with the simultaneous assessment of the expression level of the three m icroRNAs. Conclusion. The changes in the expression level of the investigated microRNAs are closely correlated with the development of PE. The quantification of plasma microRNA expression in the period after 30 weeks allows for the identification of pregnant women with PE with high accuracy.

Full Text

Restricted Access

About the authors

Ksenia N. Prozorovskaya

Email: ksenyap@inbox.ru
obstetrician-gynecologist in obstetrical observational department, Research Center of Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia. 117997, Russia, Moscow, Ac. Oparina str. 4

Leonid Z. Fayzullin

Email: l_faizullin@oparina4.ru
M.D., senior scientific researcher in the laboratory of molecular and genetic methods, Research Center of Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia 117997, Russia, Moscow, Ac. Oparina str. 4

Vitaliy N. Karnauhov

Email: v_karnauhov@oparina4.ru
junior scientific researcher in the laboratory of molecular and genetic methods, Research Center of Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia 117997, Russia, Moscow, Ac. Oparina str. 4

Natalia E. Kan

Email: kan-med@mail.ru
M.D., PhD; the head of the obstetrical observational department, Research Center of Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia 117997, Russia, Moscow, Ac. Oparina str. 4

Victor L. Tyutyunnik

Email: tioutiounnik@mail.ru
M.D., PhD; the head of the obstetric physiological department, Research Center of Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia 117997, Russia, Moscow, Ac. Oparina str. 4

Maria B. Ganichkina

Email: mariaganichkina@yandex.ru
junior scientific researcher, Research Center of Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia. 117997, Russia, Moscow, Ac. Oparina str. 4

Olga A. Sergunina

Email: o_sergunina@oparina4.ru
obstetrician-gynecologist in obstetrical observational department, Research Center of Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia. 117997, Russia, Moscow, Ac. Oparina str. 4

Andrey A. Chausov

Email: a_chausov@oparina4.ru
junior scientific researcher in the department of medical and social researches, Research Center of Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia. 117997, Russia, Moscow, Ac. Oparina str. 4

Nataliya V. Tyutyunnik

Email: tysia07@bk.ru
the resident, Research Center of Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia. 117997, Russia, Moscow, Ac. Oparina str. 4

Diana A. Mantrova

Email: d-mantrova@yandex.ru
junior scientific researcher, Research Center of Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia. 117997, Russia, Moscow, Ac. Oparina str. 4

Dmitry U. Trofimov

Email: d_trofimov@oparina4.ru
M.D., PhD, the Head of the laboratory of molecular and genetic methods, Research Center of Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia. 117997, Russia, Moscow, Ac. Oparina str. 4

References

  1. Ходжаева З.С., Коган Е.А., Клименченко Н.И., Акатьева А.С., Сафонова А.Д., Холин А.М., Вавина О.В., Сухих Г.Т. Клинико-патогенетические особенности ранней и поздней преэклампсии. Акушерство и гинекология. 2015; 1: 12-7. [Khodzhaeva Z.S., Kogan E.A., Klimenchenko N.I., Akatyeva A.S., Kholin A.M., Vavina O.V., Sukhikh G.T. Clinical and pathogenetic features of early and late preeclampsia. Akusherstvo i Ginekologiya/Obstetrics and Gynecology. 2015; (1): 12-17. (in Russian)]
  2. Ходжаева З.С., Акатьева А.С., Холин А.М., Сафонова А.Д., Вавина О.В., Муминова К.Т. Молекулярные детерминанты развития ранней и поздней преэклампсии. Акушерство и гинекология. 2014; 6: 14-9. [Khodzhaeva Z.S., Akatyeva A.S., Kholin A.M., Safonova A.D., Vavina O.V., Muminova K.T. Molecular determinants of the development of early and late preeclampsia. Akusherstvo i Ginekologiya/Obstetrics and Gynecology. 2014; 6: 14-9. (in Russian)]
  3. Brown M.A., Lindheimer M.D., de Swiet M., Van Asshe A., Moutquin J.M. The classification and diagnosis of the hypertensive disorders of pregnancy: statement from the International Society for the Study of Hypertension in Pregnancy (ISSHP). Hypertens. Pregnancy. 2001; 20(1): IX-XIV.
  4. Сидорова И.С., Никитина Н.А. Особенности патогенеза эндотелиоза при преэклампсии. Акушерство и гинекология. 2015; 1: 72-8. [Sidorova I.S., Nikitina N.A. Pathogenesis of endotheliosis in preeclampsia. Akusherstvo i ginekologiya/Obstetrics and Gynecology. 2015; (1): 72-8. (in Russian)]
  5. Долгушина В.Ф., Сюндюкова Е.Г. Особенности плацентарного апоптоза и клеточной пролиферации при преэклампсии. Акушерство и гинекология. 2015; 2: 12-9. [Dolgushina V.F., Syundyukova E.G. The specific features of placental apoptosis and cell proliferation in preeclampsia. Akusherstvo i ginekologiya/Obstetrics and Gynecology. 2015; (2): 12-9. (in Russian)]
  6. Bartel D.P. MicroRNAs: target recognition and regulatory functions. Cell. 2009; 136(2): 215-33.
  7. Baek D., Villen J., Shin C., Camargo F.D., Gygi S.P., Bartel D.P. The impact of microRNAs on protein output. Nature. 2008; 455(7209): 64-71.
  8. Griffiths-Jones S., Saini H.K., van Dongen S., Enright A.J. MiRBase: tools for microRNA genomics. Nucleic Acids Res. 2008; 36 (Database issue): D154-8.
  9. Mouillet J.F., Chu T., Sadovsky Y. Expression patterns of placental microRNAs. Birth Defects Res. A Clin. Mol. Teratol. 2011; 91(8): 737-43.
  10. Fu G., Brkic J., Hayder H., Peng C. MicroRNAs in human placental development and pregnancy complications. Int. J. Mol. Sci. 2013; 14(3): 5519-44.
  11. Higashijima A., Miura K., Mishima H., Kinoshita A., Jo O., Abe S. et al. Characterization of placenta-specific microRNAs in fetal growth restriction pregnancy. Prenat. Diagn. 2013; 33(3): 214-22.
  12. Zhu X.M., Han T., Sargent I.L., Yin G.W., Yao Y.Q. Differential expression profile of microRNAs in human placentas from preeclamptic pregnancies vs normal pregnancies. Am. J. Obstet. Gynecol. 2009; 200(6): 661. e1-7.
  13. Mouillet J.F., Ouyang Y., Coyne C.B., Sadovsky Y. MicroRNAs in placental health and disease. Am. J. Obstet. Gynecol. 2015; 213(4, Suppl.): S163-72.
  14. Tsochandaridis M., Nasca L., Toga C., Levy-Mozziconacci A. Circulating microRNAs as clinical biomarkers in the predictions of pregnancy complications. Biomed. Res. Int. 2015; 2015: 294954.
  15. Li H., Ge Q., Guo L., Lu Z. Maternal plasma miRNAs expression in preeclamptic pregnancies. Biomed. Res Int. 2013; 2013: 970265.
  16. Li P., Guo W., Du L., Zhao J., Wang Y., Liu L., Hu Y., Hou Y. MicroRNA-29b contributes to pre-eclampsia through its effects on apoptosis, invasion and angiogenesis of trophoblast cells. Clin. Sci. 2013; 124(1): 27-40.
  17. Murphy M.S., Casselman R.C., Tayade C., Smith G.N. Differential expression of plasma microRNA in preeclamptic patients at delivery and 1 year postpartum. Am. J. Obstet. Gynecol. 2015; 213(3): 367. e1-9.
  18. Doridot L., Houry D., Gaillard H., Chelbi S.T., Barbaux S., Vaiman D. Mir-34a expression, epigenetic regulation, and function in human placental diseases. Epigenetics. 2014; 9(1): 142-51.
  19. Sun M., Chen H., Liu J., Tong C., Meng T. MicroRNA-34a inhibits human trophoblast cell invasion by targeting MYC. BMC Cell Biol. 2015;16: 21.

Supplementary files

Supplementary Files
Action
1. JATS XML
Download

Copyright (c) 2016 Bionika Media

This website uses cookies

You consent to our cookies if you continue to use our website.

About Cookies