Chronic endometritis: The levels of regulatory and transport proteins in blood and intrauterine swabs in the prediction of the effectiveness of IVF programs


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Abstract

Objective. To estimate the levels of regulatory and transport proteins (alpha2-macroglobulin (а-MG), lactoferrin (LF), and albumin (ALB)) in the blood and intrauterine swabs of patients with tubal-peritoneal infertility associated with chronic endometritis (CE) for the prediction of the effectiveness of IVF programs. Subjects and methods. The samples of blood and endometrial swabs were examined in 150 patients with tubal-peritoneal infertility associated with CE (a study group) and without chronic endometritis (a comparison group) who were treated to restore fertility by IVF in the „Medica” Clinic, “Mother and Child” Group of Companies (Novokuznetsk, Russia), in relation to the effectiveness of IVF programs before and after their implementation. Blood α2-MG and ALB levels were determined by quantitative rocket immunoelectrophoresis, the content of LF in blood and swabs and that of α2-MG and ALB in swabs were estimated by solid-phase enzyme immunoassay. Results. The women with CE were found to have no considerable changes in the venous blood protein profile, regardless of the effectiveness of IVF programs. Local (intrauterine) antibacterial LF deficiency was found in women with infertility irrespective of its causes (CE or isolated tubal factor infertility). The subgroup of patients with ineffective IVF programs showed substantially elevated ALB and a2-MG levels in uterine swabs. Conclusion. The found statistically significant changes suggest that there is a local reduction in the mucosal antibacterial potential in the presence of inflammatory manifestations and concomitant exudation of ALB and α2-MG from blood flow in women with tubal-peritoneal infertility associated with CE. This allows the author to recommend the determination of LF, ALB and α2-MG in uterine swabs in CE prior to the entry into the IVF program in order to predict its effectiveness.

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About the authors

Victoria Vasilyevna Likhacheva

Novokuznetsk State Institute for Postgraduate Training of Physicians, Ministry of Health of Russia

Email: viroli@mail.ru
PhD (medical science), teaching assistant of Department of obstetrics and gynecology

Yana Nikolaevna Tretyakova

Perinatal Medical Center, «Mother and Child» Group of Companies

Email: barly@yandex.ru
obstetrician-gynecologist

Veronika Nikolayevna Zorina

Novokuznetsk State Institute for Postgraduate Training of Physicians, Ministry of Health of Russia

Email: macroglobulin@yandex.ru
D.Sc. (Doctor of biological science), Principal Research fellow of Research Laboratory of Immunology

Lyudmila Grigoryevna Bazhenova

Novokuznetsk State Institute for Postgraduate Training of Physicians, Ministry of Health of Russia

Email: l_bagenova@mail.ru
M.D. (Doctor of medical science), Professor, Head of Department of obstetrics and gynecology

Arkadiy Gennadyevich Markdorf

«Medica» Clinic, «Mother and Child» Group of Companies

Email: markdorf@mail.ru
PhD (medical science), medical director

Larisa Stepanovna Sotnikova

Siberian State Medical University

Email: sotnikova-larisa@mail.ru
M.D. (Doctor of medical science), Professor of Department of obstetrics and gynecology

Nikolay Alexeevich Zorin

Novokuznetsk State Institute for Postgraduate Training of Physicians, Ministry of Health of Russia

Email: macroglobulin@yandex.ru
D.Sc. (Doctor of biological science), Professor, Head of Research Laboratory of Immunology

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