Cervical intraepithelial neoplasia: Analysis of mRNA profile in the practice of liquid-based cytology

Nadezhda Vasilevna Melnikova; Мельникова Надежда Васильевна; Vladimir Konstantinovich Bozhenko; Боженко Владимир Константинович; Irina Borisovna Antonova; Антонова Ирина Борисовна; Nataliya Aleksandrovna Babaeva; Бабаева Наталия Александровна; Nataliya Yurevna Yarovaya; Яровая Наталия Юрьевна; Nataliya Aleksandrovna Bolotina; Болотина Наталия Александровна; Margarita Vladimirovna Zakharenko; Захаренко Маргарита Владимировна; Anna Leonidovna Senchukova; Сенчукова Анна Леонидовна; Nataliya Borisovna Akopova; Акопова Наталия Борисовна; Nataliya Vladimirovna Aleksandrova; Александрова Наталия Владимировна; Olga Vladimirovna Burmenskaya; Бурменская Ольга Владимировна; Levon Andreevich Ashrafyan; Ашрафян Левон Андреевич

doi:10.18565/aig.2017.4.95-100

Cervical intraepithelial neoplasia: Analysis of mRNA profile in the practice of liquid-based cytology

Authors: Melnikova N.V.¹, Bozhenko V.K.¹, Antonova I.B.¹, Babaeva N.A.¹, Yarovaya N.Y.¹, Bolotina N.A.¹, Zakharenko M.V.¹, Senchukova A.L.¹, Akopova N.B.¹, Aleksandrova N.V.², Burmenskaya O.V.², Ashrafyan L.A.¹
Affiliations:
1. Russian Research Center of Roentgenology and Radiology, Ministry of Health of Russia
2. Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia
Issue: No 4 (2017)
Pages: 95-100
Section: Articles
URL: https://journals.eco-vector.com/0300-9092/article/view/247903
DOI: https://doi.org/10.18565/aig.2017.4.95-100
ID: 247903

Cite item

Full Text

Open Access
Restricted Access

Access granted
Restricted Access

Subscription or Fee Access

Abstract
Full Text
About the authors
References
Supplementary files
Statistics

Abstract

Subjects and methods. The mRNA expression level of 21 genes (Ki-67, STK-15, CCNB1, CCND1, MYC, MYBL2, P16INK4A, PTEN, BIRC5, BCL2, BAG1, TERT, NDRG1, ESR1, PGR, HER2, GRB7, MGB1, MMP11, CTSL2, and CD68) was determined by quantitative PCR in the material of preservative fluid collection vials after CellPrep PAP test in 59 patients who had been treated at the Russian Research Center of Roentgenology and Radiology in 2015-2016. The test of significance was the results of comparison with subsequent histological examination. Results. Discriminant analysis has established that a combined assessment of ESR1 and MYBL2 mRNA expression levels allows correct classification in 88.24% of cases of CIN2+ changes and in less than 84.0% of cases of histologically confirmed CIN1. The combined assessment of mRNA expression levels of 17 genes (ESR1, MYBL2, CD68, PTEN, CCND1, BCL2, HER2, MMP11, TERT, STK15, P16INK4A, BAG1, CTSL2, KI67, CCNB1, GRB7, and NDRG1) permits differentiation of CIN 2+ and CIN1 or less cases with an accuracy at 98.3%. The coincidence rate of the classification with the data of histological examination was 100.0% for the CIN2+ group and 96.0% for the histologically confirmed CIN1 or less group. Conclusion. It has been shown for the first time that the mRNA expression of a large group of genes can be analyzed by quantitative PCR in the material of CellPrep liquid-based cytology in the PAP test. Assessment of the expression of a panel of 21 genes, which complements the preoperative morphological diagnosis of precancerous processes and cancer of the cervix uteri in the material of liquid-based cytology, allows a high-precision differential diagnosis of 2 clinically important groups, such as the CIN 2+ and CIN1 or less ones (intact epithelium/benign tissue changes of the cervix).

Keywords

cervical pathology, gene expression, mRNA, liquid-based cytology, cytology

Full Text

References

Каприн А.Д., Старинский В.В., Петрова Г.В., ред. Злокачественные новообразования в России в 2015 году (заболеваемость и смертность). М.: МНИОИ им. П.А. Герцена - филиал ФГБУ «НМИРЦ» Минздрава России; 2017. 250с. Available at: http://www.oncology.ru/service/statistics/ malignant_tumors/2015.pdf [Kaprin A.D., Starinsky V.V., Petrova G.V., ed. Malignant neoplasms in Russia in 2015 (morbidity and mortality). Moscow: MNIOI im. P.A. Herzen - branch of FGBU „NIIRTs” of the Ministry of Health of Russia; 2017. 250s. Available at: http://www.oncology.ru/service/ statistics/malignant_tumors/2015.pdf (in Russian)]
Боженко В.К., Кудинова Е.А., Близнюков О.П., Мельникова Н.В., Бурменская О.В. Патент на изобретение RUS 2464570, МПК G01N33/53. Метод диагностики цервикальных интраэпителиальных неоплазий. Заявитель и патентообладатель ФГУ „РНЦРР” Минздравсоцразвития России (RU). № 2010147074/15; Заявлено 19.11.2010; Опубл. 20.10.2012; Бюл. №29. 9с. [Bozhenko V.K., Kudinova E.A., Bliznyukov O.P., Melnikova N.V., Burmenskaya O.V. Patent for invention RUS 2464570, IPC G01N33 / 53. The method of diagnosis of cervical intraepithelial neoplasia. Applicant and patent holder of FGU „RNSRD” of the Ministry of Health and Social Development of Russia (RU). No. 2010147074/15; Declared on 19.11.2010; Publ. 20.10.2012; Bul. №29. 9p. (in Russian)]
Боженко В.К., Ашрафян Л.А., Антонова И.Б., Мельникова Н.В., Бурменская О.Н., Трофимов Д.Ю., Кудинова Е.А., Хунова Л.З., Слонов А.В., Близнюков О.П. Анализ экспрессии генов пролиферации и апоптоза при цервикальных интраэпителиальных неоплазиях и раке шейки матки. Опухоли женской репродуктивной системы. 2011; 4: 72-5. [Bozhenko V.K., Ashrafyan L.A., Antonova I.B., Mel’nikova N.V., Burmenskaya O.N., Trofimov D.Yu., Kudinova E.A., Hunova L.Z., Slonov A.V., Bliznyukov O.P. Analysis of the expression of proliferation and apoptosis genes in cervical intraepithelial neoplasia and cervical cancer. Opuholi zhenskoj reproduktivnoj sistemy. 2011;4:72-75 (in Russian)]
Del Pino M., Svanholm-Barrie C., Torné A., Marimon L., Gaber J., Sagasta A. et al. mRNA biomarker detection in liquid-based cytology: a new approach in the prevention of cervical cancer. Mod. Pathol. 2015; 28(2): 312-20. doi: 10.1038/ modpathol.2014.106.
Wang H.Y., Park S., Kim S., Lee D., Kim G., Kim Y. et al. Use of hTERT and HPV E6/E7 mRNA RT-qPCR TaqMan assays in combination for diagnosing high-grade cervical lesions and malignant tumors. Am. J. Clin. Pathol. 2015; 143(3): 344-51. doi: 10.1309/AJCPF2XGZ2XIQYQX.
Боженко В.К., Васкевич Е.Ф., Трофимов Д.Ю., Бурменская О.В., Кудинова Е.А., Хунова Л.З. Анализ изменения профиля экспрессии генов при гиперпролиферативных заболеваниях шейки матки. Клиническая лабораторная диагностика. 2011; 9: 21. [Bozhenko V.K., Vaskevich E.F., Trofimov D.Yu., Burmenskaya O.V., Kudinova E.A., Hunova L.Z. Analysis of changes in the profile of gene expression in hyperproliferative diseases of the cervix. Klinicheskaya laboratornaya diagnostika. 2011;9: 21 (in Russian)]
Tavassoli F.A., Devilee P., eds. Pathology and genetics of tumours of the breast and female genital organs. Lyon: IARC; 2003. 432p.
Zhai Y., Bommer G.T., Feng Y., Wiese A.B., Fearon E.R., Cho K.R. Loss of estrogen receptor 1 enhances cervical cancer invasion. Am. J. Pathol. 2010; 177(2): 884-95. doi: 10.2353/ajpath.2010.091166.
Astbury K., McEvoy L., Brian H., Spillane C., Sheils O., Martin C. et al. MYBL2 (B-MYB) in cervical cancer: putative biomarker. Int. J. Gynecol. Cancer. 2011;21(2): 206-12. doi: 10.1097/IGC.0b013e318205759f.

Supplementary files

Supplementary Files

Action

1. JATS XML

Download

Username
Password
Remember me

Forgot password?	Register

Username
Password
Remember me

Forgot password?	Register

Cervical intraepithelial neoplasia: Analysis of mRNA profile in the practice of liquid-based cytology

Full Text

Abstract

Keywords

Full Text

About the authors

References

Supplementary files

This website uses cookies