Characteristics of the phenotype of peripheral blood lymphocytes in women with idiopathic recurrent miscarriage in relation to the outcome of pregnancy during immunocytotherapy


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Abstract

Objective. To retrospectively analyze the subpopulation composition of peripheral blood lymphocytes in patients with idiopathic recurrent miscarriage (RM) with different outcomes in the first trimester of pregnancy occurring after pregestational alloimmunization. Subjects and methods. The lymphocyte phenotype was determined in 36patients with RM in non-pregnant and pregnant states during alloimmunization. Pregnancy in 30 patients resulted in the birth of a baby; 6 patients lost pregnancy. Results. In the non-pregnant state, the patients with miscarriage had lower levels of NK cells. At 5-6 weeks’ gestation, the decrease in the content of CD3-CD16+, CD56+, CD45+CD56,16+ lymphocyte subpopulations below the threshold with 100% specificity can predict miscarriage. Conclusion. The obtained result confirms the need for the proinflammatory state of the female immune system in the early stages of gestation to prolong pregnancy and enables the elaboration of approaches to choosing immunomodulatory therapy for patients with idiopathic RM.

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About the authors

Lyubov V. Krechetova

Research Center of Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia

Email: k_l_v_@mail.ru
Ph.D. in medical sciences, Head of Laboratory of Clinical Immunology

Nana K. Tetruashvili

Research Center of Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia

Email: tetrauly@mail.ru
Doctor of Medicine, Head of the Department of Pregnancy Loss Prevention and Therapy

Valentina V. Vtorushina

Research Center of Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia

Email: vtorushina@inbox.ru
Ph.D. in medical sciences, doctor of laboratory diagnostics in Laboratory of Clinical Immunology

Marina A. Nikolaeva

Research Center of Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia

Email: nikolaeva_ma@mail.ru
Doctor of Science, Leading researcher, Laboratory of Clinical Immunology

Nelly A. Khachatryan

Research Center of Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia

Email: nellyl986@mail.ru
Graduate student, Department of Pregnancy Loss Prevention and Therapy

Anna Aramovna Agadzhanova

Research Center of Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia

Email: aagadjanova@mail.ru
Doctor of Medicine, Doctor of Department of Pregnancy Loss Prevention and Therapy

L. V Vanko

Research Center of Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia

Email: l_Vanko@oparina4.ru
doctor of medicine, professor, leading research worker of clinical immunology laboratory

Tatiana Y. Ivanets

Research Center of Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia

Email: t_ivanets@oparina4.ru
PhD. in medical sciences, Head of Clinical Diagnostic Laboratory

Gennady T. Sukhikh

Research Center of Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia

MD, PhD, Academician of Russian Academy of Sciences, Professor, Director

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