Study of gene expressions in the eutopic endometrium of women with endometrioid ovarian cysts


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Abstract

Objective. To identify the genes, the expression of which in the eutopic proliferative endometrium differs in patients with endometrioid ovarian cysts (EOC) compared to that in the eutopic proliferative endometrium of women without endometriosis. Subjects and methods. Two patient groups (10 women with EOC (a study group) and 10 women without endometriosis (a control group)) were examined. All the patients underwent a full clinical-anamnestic examination: history data collection, general physical and gynecological examinations, pelvic ultrasonography, clinical and laboratory examination. Eutopic endometrial tissue pipelle biopsy specimens were obtained directly intraoperatively before hysteroscopy, with subsequent endometrectomy and histological examination. Total RNA was isolated from the specimens; an expression prof ile was subsequently obtained by microarray hybridization using the GeneChip Human Exon 1.0 ST Arrays (Affymetrix, USA) according to the manufacturer’s protocol. Results. The eutopic proliferative endometrium of the women with EOC (a study group) showed increased expression of 27 genes and decreased expression of 17 genes (the microarray measures the expression level of a total of 14318 transcripts) as compared with that from the women without endometriosis (a control group). All these genes are encountered in the works on eutopic endometrial expression; thus, our results are largely consistent with the published data, which makes these genes promising biomarkers for endometriosis. Conclusion. The obtained data on the increased expression of the FOS, EGR-1, FOSB, DUSP1, ZFP36, JUNB, and JUN genes in the eutopic proliferative endometrium of women with EOC agree with the results of previously published works, which makes these genes promising biomarkers to create real-time PCR-based diagnostic systems for the determination of mRNA levels in endometrial tissues.

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About the authors

Maria Vladimirovna Kuznetsova

Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation

Email: mkarja@mail.ru
Ph.D, research scientist, Laboratory of molecular-genetic methods

Ekaterina Yuryevna Pshenichnyuk

Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation

Email: best-kauty@mail.ru
PhD-student, Department of Surgical Gynecology

Olga Vladimirovna Burmenskaya

Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation

Email: bourmenska@mail.ru
Ph.D., research scientist, Laboratory of molecular-genetic methods

Alexandra Vyacheslavovna Asaturova

Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation

Email: a_asaturova@oparina4.ru
PhD, senior scientific researcher of pathology department

Dmitry Yurievich Trofimov

Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation

Email: d_troflmov@oparina4.ru
DSc, Head of Laboratory of molecular-genetic methods

Leila Vagoevna Adamyan

Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation

Email: l_adamyan@oparina4.ru
MD, professor, academician of RAS, deputy director on science, head of department of operative gynecology

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