Time course of changes in the production of anti-leukocyte antibodies in pregnant women with idiopathic recurrent miscarriage during immunocytotherapy


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Abstract

Objective. To reveal regularities in the development of humoral immune responses during pregestational and gestational immunocytotherapy (ICT) in patients with recurrent miscarriage (RM), by estimating the level of antipaternal anti-leukocyte antibodies (anti-PAb). Subjects and methods. Anti-PAbs were determined by cross-reactivity using flow cytometry at different stages of gestation in 85 control pregnant women and in 92 women with RM, among whom there were 69 women who received pregravid preparation (PP) involving immunization with their partner’s lymphocytes (ICT) as monotherapy or in combination with oral dydrogesterone, and 23 who had no PP. Results. In the controls, anti-PAbs were detected in 5.9-7% of the pregnant women at less than 34 weeks’ gestation and in 25.9% at 38 weeks. The level of anti-PAbs at 5 to 6 weeks of gestation was signif icantly higher in the patients who received PP including ICT. In the patients who had no PP, the anti-PAB levels gradually increased after each immunization procedure in the first trimester to the level seen in those who received PP. Conclusion. The emergence of anti-PAbs during pregnancy in patients with RM is the result of ICT, which indicates the importance of an anti-PAb test that reflects the immunomodulatory effect of an alloimmunization procedure.

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About the authors

Lyubov V. Krechetova

Research Center of Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia

Email: k_l_v_@mail.ru
Ph.D. in medical sciences, Head of Laboratory of Clinical Immunology

Nana K. Tetruashvili

Research Center of Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia

Email: n_tetruashvili@oparina4.ru
Doctor of Medicine, Head of the Department of Pregnancy Loss Prevention and Therapy

Valentina V. Vtorushina

Research Center of Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia

Email: vtorushina@inbox.ru
Ph.D. in medical sciences, doctor of laboratory diagnostics in Laboratory of Clinical Immunology

Marina A. Nikolaeva

Research Center of Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia

Email: nikolaeva_ma@mail.ru
Doctor of Science, Leading researcher, Laboratory of Clinical Immunology

Elena L. Golubeva

Research Center of Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia

Email: e_golubeva@oparina4.ru
Ph.D. in medical sciences, doctor of laboratory diagnostics in Laboratory of Clinical Immunology

Victoria A. Saribegova

Research Center of Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia

Email: saribegova_v@rambler.ru
Graduate student, Department of Pregnancy Loss Prevention and Therapy

Anna Aramovna Agadzhanova

Research Center of Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia

Email: aagadjanova@mail.ru
Doctor of Medicine, Doctor of Department of Pregnancy Loss Prevention and Therapy

L. V Vanko

Research Center of Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia

Email: LVanko@oparina4.ru
doctor of medicine, professor, leading research worker of clinical immunology laboratory

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