Fetal growth restriction and markers for mitochondrial dysfunction


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Abstract

Objective. To comparatively analyze the content of mitochondrial components in the placenta and whole blood of women with physiological and fetal growth restriction-complicated pregnancy. Subjects and methods. Examinations were made in 32 women (10 patients with physiological pregnancy and 12 patients with diagnosed fetal growth restriction) starting at 24 weeks’ gestation. The levels of OPA1, DRP1, VDAC1, TFAM, and hOGG1 proteins were estimated using chemiluminescence western blotting. The blood levels of mitochondrial DNA copy number were determined by real-time PCR. Results. There was an increased hOGG1 protein content without changes in the levels of mitochondrial fusion and fragmentation factors in the placental tissue of the examined groups. There was a significant reduction in mitochondrial DNA abundance at 25, 29, and 37 weeks’ gestation in the blood of patients with fetal growth restriction compared to the similar weeks of physiological pregnancy. Conclusion. There are pronounced manifestations of oxidative stress in fetal growth restriction-complicated pregnancy. The reduction in the maternal blood mitochondrial DNA abundance in fetal growth restriction may be suggestive of obvious mitochondrial dysfunction in the placenta and be used as a prognostic marker for this pathology

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About the authors

Polina A. Vishnyakova

National Medical Research Center of Obstetrics, Gynecology, and Perinatology named after Academician V.I. Kulakov, Ministry of Health of Russia

Email: p_vishnyakova@oparina4.ru
PhD, Researcher, Laboratory of mitochondrial medicine

Iuliia A. Sukhanova

National Medical Research Center of Obstetrics, Gynecology, and Perinatology named after Academician V.I. Kulakov, Ministry of Health of Russia

Email: suhanova_julia@hotmail.com
Researcher, Laboratory of mitochondrial medicine

Asmik G. Mikaelyan

National Medical Research Center of Obstetrics, Gynecology, and Perinatology named after Academician V.I. Kulakov, Ministry of Health of Russia

Email: mikaelyan_asmik@bk.ru
Graduate student, Department of pregnancy loss prevention and therapy

Yulia S. Bulatova

National Medical Research Center of Obstetrics, Gynecology, and Perinatology named after Academician V.I. Kulakov, Ministry of Health of Russia

Email: yu.bulatova@mail.ru
Graduate student, Department of pregnancy loss prevention and therapy

Sofia V. Pyataeva

National Medical Research Center of Obstetrics, Gynecology, and Perinatology named after Academician V.I. Kulakov, Ministry of Health of Russia

Email: biosonya@gmail.com
PhD, Researcher, Laboratory of mitochondrial medicine

Ivan S. Balashov

National Medical Research Center of Obstetrics, Gynecology, and Perinatology named after Academician V.I. Kulakov, Ministry of Health of Russia

Email: i_balashov@oparina4.ru
Expert, the Bioinformatics research group

Pavel I. Borovikov

National Medical Research Center of Obstetrics, Gynecology, and Perinatology named after Academician V.I. Kulakov, Ministry of Health of Russia

Email: p_borovikov@oparina4.ru
Head of the Bioinformatics research group

Nana K. Tetruashvili

National Medical Research Center of Obstetrics, Gynecology, and Perinatology named after Academician V.I. Kulakov, Ministry of Health of Russia

Email: tetrauly@mail.ru
M.D., PhD, Head of the Department of pregnancy loss prevention and therapy

Mikhail Y. Vyssokikh

National Medical Research Center of Obstetrics, Gynecology, and Perinatology named after Academician V.I. Kulakov, Ministry of Health of Russia

Email: m_vysokikh@oparina4.ru
PhD, Head of the Mitochondrial medicine research group

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