Prevalence of HPV types in patients with cervical intraepithelial neoplasia of different severity

Objective. To study the prevalence of HPV (human papillomavirus) types in patients with squamous intraepithelial lesions (SIL) of different severity and cervical cancer in comparative perspective. Subjects and methods. This prospective study included 145 women aged 18 to 49 years. The survey consisted of: collecting complaints, anamnesis, gynecological status, conducting extended colposcopy, molecular biological methods (multiplex PCR with detection results in real-time for quantification and typing of HPV 21 types), cytological examination, histological analysis of biopsy material. 5 groups were formed including 145 women: 31 patients (21,3%) - NILM (I group), 30 patients (20,6%) - chronic cervicitis (histologically confirmed) (group II), 37 patients (22,7%) - LSlL (III group), 31 patients (21,3%) - HSIL (IV group ) and 14 patients (9,6%) - cervical cancer (V group). Results. The most significant risk factors for cervical intraepithelial lesions are: early sexual initiation, great number of sexual partners (>4), sexual transmitted infections (STI) history. The most frequent HPV types were 16, 31, 33 and 51 types. We demonstrated that HPV type 16 was significantly more prevalent in the group with HSIL and squamous cervical cancer compared to group II (chronic cervicitis) and group III (LSIL). HPV31 and 33 types are more prevalent in the group with HSIL. According to our results, there is the prevalence of the group A9 HPV in women with LSIL, HSIL and cervical cancer compared to group II (chronic cervicitis). Conclusion. Our results demonstrate the key role of A9 HPV group in the development of premalignant and malignant cervical disease. It should also be noted that in patients with LSIL in addition to A9 HPV group there is a trend to increase the incidence of HPV groups A7, A10, A5.

human papillomavirus, chronic cervicitis, cervical intraepithelial neoplasia, cervical cancer, real time PCR