Characteristics of coagulase-negative Staphylococci isolated from central venous catheters of neonates in an intensive care unit: formation of biofilm and genetic population structure identified by multilocus sequencing typing


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Abstract

Aim. To investigate the ability of hospital-associated populations of S. epidermidis to form biofilms on the inner surface of central venous catheters (CVC). Material and methods. The study analyzed 150 samples of CVCs obtained after catheterization of neonates. Results. Fifty three catheters were colonized by conditional pathogens, and 50 samples were contaminated by a single strain. Isolates of S. epidermidis were present in 68% of samples. They were assigned to 12 different sequence types, most frequently to ST59 (19 isolates, 56%). The icaADBC operon genes, which are involved in the production of the polysaccharide intercellular adhesin (PIA), were not detected in the majority of isolates. Conclusion. Most of CVCs were colonized by S. epidermidis (62% of isolates) with sequence types belonging to the CC2 clonal complex, thus indicating that they are hospital acquired. A group of isolates with an increased ability to adhere to plastics was identified. Some isolates were found to be ica-operon negative, but capable of forming a biofilm on the catheter surface by an ica-independent mechanism.

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About the authors

Maria A. Kornienko

Federal Research and Clinical Center of Physical and Chemical Medicine, Federal Biomedical Agency of Russia

Email: kornienkomariya@gmail.com
PhD, Junior researcher in the Laboratory of molecular genetics of microorganisms, Federal Research and Clinical Center of Physical and Chemical Medicine

Nikita S. Kuptsov

Federal Research and Clinical Center of Physical and Chemical Medicine, Federal Biomedical Agency of Russia

assistant in the Laboratory of molecular genetics of microorganisms

Roman B. Gorodnichev

Federal Research and Clinical Center of Physical and Chemical Medicine, Federal Biomedical Agency of Russia

Junior researcher in the Laboratory of molecular genetics of microorganisms

Maya V. Malakhova

Federal Research and Clinical Center of Physical and Chemical Medicine, Federal Biomedical Agency of Russia

Researcher in the Laboratory of molecular genetics of microorganisms

Lyudmila A. Lyubasovskaya

National Medical Research Center of Obstetrics, Gynecology, and Perinatology named after Academician V.I. Kulakov, Ministry of Health of Russia

Email: labmik@yandex.ru
PhD, Head of the Unit of clinical pharmacology, Department of microbiology and clinical pharmacology

Aleksey B. Gordeev

National Medical Research Center of Obstetrics, Gynecology, and Perinatology named after Academician V.I. Kulakov, Ministry of Health of Russia

Email: gordeew@vega.protres.ru
Senior Researcher in the Laboratory of microbiology, Department of microbiology and clinical pharmacology

Irina V. Nikitina

National Medical Research Center of Obstetrics, Gynecology, and Perinatology named after Academician V.I. Kulakov, Ministry of Health of Russia

Email: i_nikitina@oparina4.ru
Senior Researcher in the Unit of resuscitation and intensive care of newborns, Department of neonatology and pediatrics

Georgy B. Smirnov

Federal Research and Clinical Center of Physical and Chemical Medicine, Federal Biomedical Agency of Russia

Chief Scientific Officer in the Laboratory of molecular genetics of microorganisms

Egor A. Shitikov

Federal Research and Clinical Center of Physical and Chemical Medicine, Federal Biomedical Agency of Russia

Senior researcher in the Laboratory of molecular genetics of microorganisms

Tatyana V. Priputnevich

National Medical Research Center of Obstetrics, Gynecology, and Perinatology named after Academician V.I. Kulakov, Ministry of Health of Russia

Email: priput1@gmail.com
Head of the Department of microbiology and clinical pharmacology

Elena N. Ilyina

Federal Research and Clinical Center of Physical and Chemical Medicine, Federal Biomedical Agency of Russia

Deputy director for science, Federal Research and Clinical Center of Physical-chemical medicine of Federal Research and Clinical Center of Physical and Chemical Medicine

References

  1. Gahlot R., Nigam C., Kumar V., Yadav G., Anupurba S. Catheter-related bloodstream infections. Int. J. Crit. Illn. Inj. Sci. 2014; 4(2): 162-7
  2. Maki D.G., Kluger D.M., Crnich C.J. The risk of bloodstream infection in adults with different intravascular devices: a systematic review of 200 published prospective studies. Mayo. Clin. Proc. 2006; 81(9): 1159-71.
  3. Otto M. Staphylococcus epidermidis - the “accidental” pathogen. Nat. Rev. Microbiol. 2009; 7(8): 555-67.
  4. Seifert H., Cornely O., Seggewiss K., Decker M., Stefanik D., Wisplinghoff H. Bloodstream infection in neutropenic cancer patients related to short-term nontunnelled catheters determined by quantitative blood cultures, differential time to positivity, and molecular epidemiological typing with pulsed-field gel electrophoresis. J. Clin. Microbiol. 2003; 41(1): 118-23.
  5. Donelli G., Vuotto C. Biofilm-based infections in long-term care facilities. Future Microbiol. 2014; 9(2): 175-88.
  6. Flemming H.C., Wingender J., Szewzyk U., Steinberg P., Rice S.A., Kjelleberg S. Biofilms: an emergent form of bacterial life. Nat. Rev. Microbiol. 2016; 14(9): 563-75.
  7. Costerton J.W., Stewart P.S., Greenberg E.P. Bacterial biofilms: a common cause of persistent infections. Science. 1999; 284(5418): 1318-22.
  8. Fey P.D., Olson M.E. Current concepts in biofilm formation of Staphylococcus epidermidis. Future Microbiol. 2010; 5(6): 917-33.
  9. Gerke C., Kraft A., Süssmuth R., Schweitzer O., Gotz F. Characterization of the N-acetylglucosaminyl transferase activity involved in the biosynthesis of the Staphylococcus epidermidis polysaccharide intercellular adhesion. J. Biol. Chem. 1998; 273(29): 18586-93.
  10. Patti J.M., Allen B.L., McGavin M.J., Hook M. MSCRAMM-mediated adherence of microorganisms to host tissues. Annu. Rev. Microbiol. 1994; 48: 585-617.
  11. Любасовская Л.А., Припутневич Т.В., Никитина И.В., Корниенко М.А., Родченко Ю.В., Гордеев А.Б., Мелкумян А.Р., Зубков В.В., Ильина Е.Н., Дубоделов Д.В., Кондрахин А.П., Сафоновская А.А. Колонизация центрального венозного катетера у новорожденных ОРИТ: факторы риска и клиническая значимость. Акушерство и гинекология. 2016; 12: 114 20. http://dx.doi.org/10.18565/aig.2016.12.114-20
  12. Miragaia M., Thomas J.C., Couto I., Enright M.C., de Lencastre H. Inferring a population structure for Staphylococcus epidermidis from multilocus sequence typing data. J. Bacteriol. 2007; 189(6): 2540-52.
  13. Корниенко М.А., Ильина Е.Н., Боровская А.Д., Эдельштейн M.В., Сухорукова M.В., Кострцева М., Говорун В.М. Прямое бактериальное профилирование посредством MALDI масс-спектрометрии как метод быстрой классификации штаммов Staphylococcus aureus. Биомедицинская химия. 2012; 58(5): 501-13.
  14. Arciola C.R., Baldassarri L., Montanaro L. Presence of icaA and icaD genes and slime production in a collection of staphylococcal strains from catheter-associated infections. J. Clin. Microbiol. 2001; 39(6): 2151-6.
  15. Arciola C., Gamberini S., Campoccia D., Visai L., Speziale P., Baldassarri L., Montanaro L. A multiplex PCR method for the detection of all five individual genes of ica locus in Staphylococcus epidermidis. A survey on 400 clinical isolates from prosthesis-associated infections. J. Biomed. Mater. Res. A. 2005; 75(2): 408-13.
  16. Корниенко М.А., Копыльцов В.Н., Шевлягина Н.В., Диденко Л.В., Любасовская Л.А., Припутневич Т.В., Ильина Е.Н. Способность стафилококков различных видов к образованию биопленок и их воздействие на клетки человека. Молекулярная генетика, микробиология и вирусология. 2016; 1: 18-25.
  17. Christensen G.D., Simpson W.A., Younger J.J., Baddour L.M., Barrett F.F., Melton D.M., Beachey E.H. Adherence of coagulase-negative staphylococci to plastic tissue culture plates: a quantitative model for the adherence of staphylococci to medical devices. J. Clin. Microbiol. 1985; 22(6): 996-1006.
  18. Polin R.A., Saiman L. Nosocomial infections in the Neonatal Intensive Care Unit. NeoReviews. 2003; 4(3): e81-9.
  19. Figueiredo A., Ferreira F., Beltrame C., Côrtes M. The role of biofilms in persistent infections and factors involved in ica-independent biofilm development and gene regulation in Staphylococcus aureus. Crit. Rev. Microbiol. 2017; 43(5): 602-20.

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