The role of methylentetrahydrofolate reductase (MTHFR) gene polymorphism in intrauterine growth restriction development


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Objective. To def ine the role of methylenetetrahydrofolate reductase polymorphism in intrauterine growth restriction (IUGR) development. Material and methods. 248 patients were involved to the case-control study. Group I included patients with IUGR, group II included 148 patients with no signs IUGR. DNA isolation was carried out by the R. Higuchi method with some modifications. The identification of a single nucleotide polymorphism was performed by means of modified method of «adjacent probes» (kissing probes), original oligonucleotides were used. MTHFR gene polymorphic loci identification was performed: 677 С > T (Ala222Val). Results. The clinical risk factors of IUGR development: medium degree myopia (20,0%) with astigmatism, mitral valve prolapse (MVP) (19.0% ), varicose veins (23,0%). IUGR pregnancy is complicated by morning sickness (32%), threatened abortion (48%) with retrochorial hematoma formation (7.0%), istmicocervical insufficiency (17,0%). IUGR development is associated with MTHFR gene polymorphism: 677 C> T (Ala222Val). Conclusion. We showed that it is reasonable to use molecular genetic investigation to identify MTHFR gene polymorphism in the group of high risk pregnancy. Folic acid is showed efficiency in IUGR prophylaxis.

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Sobre autores

Victor Tyutyunnik

National Medical Research Center for Obstetrics, Gynecology and Perinatology named after Academician V.l. Kulakov Ministry of Health of Russia

Email: tioutiounnik@mail.ru
PhD, MD, the head of the obstetric physiological department

Natalia Kan

National Medical Research Center for Obstetrics, Gynecology and Perinatology named after Academician V.l. Kulakov Ministry of Health of Russia

Email: kan-med@mail.ru
PhD, MD, the head of the obstetric department

Diana Mantrova

National Medical Research Center for Obstetrics, Gynecology and Perinatology named after Academician V.l. Kulakov Ministry of Health of Russia

Email: d_mantrova@oparina4.ru
graduate student of the obstetric department

Natalia Lomova

National Medical Research Center for Obstetrics, Gynecology and Perinatology named after Academician V.l. Kulakov Ministry of Health of Russia

Email: n_lomova@oparina4.ru
PhD, scientific researcher of the obstetric department

Igor Klimantsev

National Medical Research Center for Obstetrics, Gynecology and Perinatology named after Academician V.l. Kulakov Ministry of Health of Russia

Email: i_klimantsev@oparina4.ru
scientific researcher of the obstetric physiological department

Andrey Donnikov

National Medical Research Center for Obstetrics, Gynecology and Perinatology named after Academician V.l. Kulakov Ministry of Health of Russia

Email: a_donnikov@oparina4.ru
PhD, the head of the Laboratory of molecular genetic methods

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